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Establishment of new omics approach for the comprehensive analysis of the light sensing pathways

Research Project

Project/Area Number 15H05578
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Genome biology
Research InstitutionToyohashi University of Technology

Principal Investigator

Hirose Yuu  豊橋技術科学大学, 工学(系)研究科(研究院), 助教 (30616230)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2015: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Keywords次世代シークエンサー / フォトーム / 光受容体 / シアノバクテリア / 藻類 / ゲノム / オプトジェネティクス / フィトクロム / 光合成 / 光応答 / 光スイッチ / トランスクリプトーム
Outline of Final Research Achievements

To understand the light-sensing network of living organisms, I developed new omics method named "Photome". This method utilizes the advantage of wide range of quantification of the next generation sequencer and the irradiation of multichromatic light with high resolution wavelength. I succeeded to establish highly efficient sample preparation method of photome and also automated pipeline for computing. This method can be applied to all living organism and will promote the progress of the wide research filed such as analysis of intrinsic light-sensing pathway or regulation of cellular response by light irradiation.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (13 results)

All 2018 2017 2016

All Journal Article (5 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (5 results) (of which Invited: 1 results) Book (1 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Characterization of the Genuine Type 2 Chromatic Acclimation in the Two Geminocystis Cyanobacteria.2017

    • Author(s)
      Hirose Y., Misawa N., Yonekawa C., Nagao N., Watanabe M., Ikeuchi M. and Eki T.
    • Journal Title

      DNA Res.

      Volume: 5(14) Issue: 4 Pages: 387-396

    • DOI

      10.1093/dnares/dsx011

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Diverse molecular processes of chromatic acclimation in the cyanobacteria phylum.2017

    • Author(s)
      Hirose Y., Ikeuchi M. and Eki T.
    • Journal Title

      Plant Morphol.

      Volume: 印刷中

    • NAID

      130006647120

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Complete genome sequence of cyanobacterium Nostoc sp. NIES-3756, a potentially useful strain for phytochrome-based bioengineering.2016

    • Author(s)
      Hirose Y., Fujisawa T., Ohtsubo Y., Katayama M., Misawa N., Wakazuki S., Shimura Y., Nakamura Y., Kawachi M., Yoshikawa H., Eki T., and Kanesaki Y.
    • Journal Title

      Journal of Biotechnology

      Volume: 220 Pages: 51-52

    • DOI

      10.1016/j.jbiotec.2015.12.002

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Complete genome sequencing of cyanobacterium Leptolyngbya sp. NIES-3755 Journal of Biotechnology.2016

    • Author(s)
      Hirose Y., Fujisawa T., Ohtsubo Y., Katayama M., Misawa N., Wakazuki S., Shimura Y., Nakamura Y., Kawachi M., Yoshikawa H., Eki T., and Kanesaki Y.
    • Journal Title

      Genome Announcements

      Volume: 4 Issue: 2

    • DOI

      10.1128/genomea.00090-16

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Complete genome sequence of cyanobacterium Fischerella sp. NIES-3754, providing thermoresistant optogenetic tools.2016

    • Author(s)
      Hirose Y., Fujisawa T., Ohtsubo Y., Katayama M., Misawa N., Wakazuki S., Shimura Y., Nakamura Y., Kawachi M., Yoshikawa H., Eki T., and Kanesaki Y.
    • Journal Title

      Journal of Biotechnology

      Volume: 220 Pages: 45-46

    • DOI

      10.1016/j.jbiotec.2016.01.011

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 大腸菌の光応答の解析2018

    • Author(s)
      広瀬侑
    • Organizer
      第12回長野ミーティング
    • Related Report
      2017 Annual Research Report
  • [Presentation] Leptolyngbya 属シアノバクテリアにおけるフィコエリスロシアニン調節型の補色順化の解析2017

    • Author(s)
      広瀬侑、長尾信義、米川千夏、渡辺麻衣、池内昌彦、浴俊彦
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学郡元キャンパス
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Leptolyngbya 属シアノバクテリアの光合成装置の光色応答の解析2017

    • Author(s)
      広瀬侑、長尾信義、米川千夏、渡辺麻衣、池内昌彦、浴俊彦
    • Organizer
      第11回日本ゲノム微生物学会年会
    • Place of Presentation
      慶應義塾大学湘南藤澤キャンパス
    • Year and Date
      2017-03-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] シアノバクテリアのゲノム解析とそれを活用した補色応答現象の理解2016

    • Author(s)
      広瀬 侑、池内 昌彦、浴俊彦
    • Organizer
      第80回日本植物学会年会
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] シアノバクテリアGeminocystis sp. NIES-3708 および3709 株のゲノム比較2016

    • Author(s)
      広瀬侑、大坪嘉行、兼崎友、藤澤貴智、志村遥平、三澤直美、若月幸子、中村保一、河地正伸、池内昌彦、浴俊彦
    • Organizer
      第7回日本光合成学会年会
    • Place of Presentation
      東京理科大学葛飾キャンパス
    • Year and Date
      2016-05-27
    • Related Report
      2016 Annual Research Report
  • [Book] 生物の科学「遺伝」(微細藻類の多様な魅力:分類,進化,ゲノムから形態,藻類バイオまで)2017

    • Author(s)
      広瀬 侑、池内 昌彦、浴俊彦
    • Publisher
      NTS出版
    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] 多波長光照射装置2017

    • Inventor(s)
      広瀬 侑
    • Industrial Property Rights Holder
      広瀬 侑
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 核酸分子およびこれを用いたタンパク質の製造方法2017

    • Inventor(s)
      広瀬侑
    • Industrial Property Rights Holder
      広瀬侑
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017-03-01
    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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