| Budget Amount *help |
¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Fiscal Year 2017: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2016: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2015: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
|
|---|
| Outline of Final Research Achievements |
PIWI-interacting RNAs (piRNAs) are germline-specific small RNAs, which form the effector complexes with PIWI proteins to preserve the genome integrity by repressing transposable elements (TEs). Among PIWI-clade proteins in Drosophila, Piwi is known to transcriptionally silence their targets via heterochromatin formation. In this study, following points were analyzed in order to reveal the molecular mechanism of RNA silencing by Piwi-piRNAs: how piRNAs are assigned to three different types of PIWI proteins, how heterochromatin is induced by Piwi-piRNAs, and how Piwi-piRNA complex is guided to their target locus. The results obtained from these analyses revealed the mechanism how small RNAs and chromatin factors can recognize self and non-self in the genome.
|
