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Recognition of self and non-self in the genome by small RNAs and chromatin factors

Research Project

Project/Area Number 15H05583
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field System genome science
Research InstitutionKeio University

Principal Investigator

Iwasaki Yuka  慶應義塾大学, 医学部(信濃町), 講師 (80612647)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Fiscal Year 2017: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2016: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2015: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Keywords小分子RNA / piRNA / トランスポゾン / 転写制御 / エピゲノム制御 / 非コードRNA / RNAサイレンシング / RNA結合タンパク質 / クロマチン因子 / small RNA / RNA-タンパク質複合体 / ゲノム安定性 / Piwi-piRNA複合体 / 標的認識 / リンカーヒストン
Outline of Final Research Achievements

PIWI-interacting RNAs (piRNAs) are germline-specific small RNAs, which form the effector complexes with PIWI proteins to preserve the genome integrity by repressing transposable elements (TEs). Among PIWI-clade proteins in Drosophila, Piwi is known to transcriptionally silence their targets via heterochromatin formation. In this study, following points were analyzed in order to reveal the molecular mechanism of RNA silencing by Piwi-piRNAs: how piRNAs are assigned to three different types of PIWI proteins, how heterochromatin is induced by Piwi-piRNAs, and how Piwi-piRNA complex is guided to their target locus. The results obtained from these analyses revealed the mechanism how small RNAs and chromatin factors can recognize self and non-self in the genome.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (29 results)

All 2018 2017 2016 2015

All Journal Article (15 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 11 results,  Open Access: 9 results,  Acknowledgement Compliant: 8 results) Presentation (14 results) (of which Int'l Joint Research: 8 results,  Invited: 4 results)

  • [Journal Article] Hierarchical roles of mitochondrial Papi and Zucchini in Bombyx germline piRNA biogenesis2018

    • Author(s)
      Nishida Kazumichi M.、Sakakibara Kazuhiro、Iwasaki Yuka W.、Yamada Hiromi、Murakami Ryo、Murota Yukiko、Kawamura Takeshi、Kodama Tatsuhiko、Siomi Haruhiko、Siomi Mikiko C.
    • Journal Title

      Nature

      Volume: 555 Issue: 7695 Pages: 260-264

    • DOI

      10.1038/nature25788

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Profiling Open Chromatin Structure in the Ovarian Somatic Cells Using ATAC-seq2018

    • Author(s)
      Murano K, Iwasaki YW, Siomi H.
    • Journal Title

      Methods in Molecluar Biology

      Volume: 1680 Pages: 165-177

    • DOI

      10.1007/978-1-4939-7339-2_11

    • ISBN
      9781493973385, 9781493973392
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deep sequencing and high-throughput analysis of PIWI-associated small RNAs2017

    • Author(s)
      Iwasaki Yuka W.、Ishino Kyoko、Siomi Haruhiko
    • Journal Title

      Methods

      Volume: 126 Pages: 66-75

    • DOI

      10.1016/j.ymeth.2017.05.020

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 内在性小分子RNAの網羅的解析手法2017

    • Author(s)
      岩崎由香
    • Journal Title

      実験医学(エピジェネティクス実験スタンダード)

      Volume: - Pages: 311-322

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Profiling open chromatin structure in the Ovarian Somatic Cells using ATAC-seq2017

    • Author(s)
      Murano K, Iwasaki YW, Siomi H
    • Journal Title

      Methods in Molecular Biology

      Volume: 印刷中

    • Related Report
      2016 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] 内在性小分子RNAの網羅的解析手法2017

    • Author(s)
      岩崎由香
    • Journal Title

      実験医学

      Volume: 印刷中

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Piwi modulates chromatin accessibility by regulating multiple factors including Histone H1 to repress transposons2016

    • Author(s)
      Iwasaki YW, Murano K, Ishizu H, Shibuya A, Iyoda Y, Siomi MC, Siomi H, Saito K
    • Journal Title

      Molecular Cell

      Volume: 63 Pages: 408-419

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells2016

    • Author(s)
      Sumiyoshi T, Sato K, Yamamoto H, Iwasaki YW, Siomi H, Siomi MC
    • Journal Title

      Genes & Development

      Volume: 30 Issue: 14 Pages: 1617-1622

    • DOI

      10.1101/gad.283929.116

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] PiwiはヒストンH1を介したクロマチンの構造の制御により標的となるトランスポゾンを抑制する2016

    • Author(s)
      岩崎由香,塩見春彦,齋藤都暁
    • Journal Title

      First Author's

      Volume: - Pages: 081

    • DOI

      10.7875/first.author.2016.081

    • Related Report
      2016 Annual Research Report
    • Open Access
  • [Journal Article] Krimper Enforces an Antisense Bias on piRNA Pools by Binding AGO3 in the Drosophila Germline2015

    • Author(s)
      Sato K, Iwasaki YW (equal contribution), Shibuya A, Carninci P, Tsuchizawa Y, Ishizu H, Siomi MC, Siomi H
    • Journal Title

      Molecular Cell

      Volume: 59 Issue: 4 Pages: 553-563

    • DOI

      10.1016/j.molcel.2015.06.024

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Somatic Primary piRNA Biogenesis Driven by cis-Acting RNA Elements and trans-Acting Yb2015

    • Author(s)
      Ishizu H, Iwasaki YW, Hirakata S, Ozaki H, Iwasaki W, Siomi H, Siomi MC
    • Journal Title

      Cell Reports

      Volume: 12 Issue: 3 Pages: 429-440

    • DOI

      10.1016/j.celrep.2015.06.035

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Respective functions of two distinct Siwi complexes assembled during piRNA biogenesis in insect germ cells.2015

    • Author(s)
      Nishida, KM., Iwasaki, YW., Murota, Y., Nagao, A., Mannen, T., Kato, Y., Siomi, H., and Siomi, MC.
    • Journal Title

      Cell Reports

      Volume: 10 Issue: 2 Pages: 193-203

    • DOI

      10.1016/j.celrep.2014.12.013

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] piRNAs derived from ancient viral processed pseudogenes as transgenerational sequence-specific immune memory in mammals.2015

    • Author(s)
      Parrish NF, Fujino K, Shiromoto Y, Iwasaki YW, Ha H, Xing J, Makino A, Kuramochi-Miyagawa S, Nakano T, Siomi H, Honda T, Tomonaga K.
    • Journal Title

      RNA.

      Volume: 21 Issue: 10 Pages: 1691-703

    • DOI

      10.1261/rna.052092.115

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] PIWI-interacting RNA: Its Biogenesis and Functions2015

    • Author(s)
      Iwasaki Y.W., Siomi M.C., Siomi H.
    • Journal Title

      Annual Review of Biochemistry

      Volume: 84 Issue: 1 Pages: 405-433

    • DOI

      10.1146/annurev-biochem-060614-034258

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Tudor-domain containing proteins act to make the piRNA pathways more robust in Drosophila2015

    • Author(s)
      Sato K, Iwasaki YW, Siomi H, Siomi MC
    • Journal Title

      Fly

      Volume: 9 Issue: 2 Pages: 86-90

    • DOI

      10.1080/19336934.2015.1128599

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Germline specific regulation of transposable elements by small RNA2018

    • Author(s)
      Iwasaki YW
    • Organizer
      Interdisciplinary Science Group in Keio
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Essential function of Panoramix/Silencio-Nxf2 complex in the Drosophila Piwi-piRNA pathway2017

    • Author(s)
      Iwasaki YW, Mashiko A, Murano K, Siomi H
    • Organizer
      The 43rd Naito Conference: Noncoding RNA : Biology, Chemistry, & Diseases
    • Place of Presentation
      シャトレーゼ ガトーキングダム サッポロ(札幌)
    • Year and Date
      2017-06-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Essential function of Panoramix/Silencio-Nxf2 complex in the Drosophila Piwi-piRNA pathway2017

    • Author(s)
      Iwasaki YW, Mahiko A, Murano K, Siomi H
    • Organizer
      The 43rd Naito Conference: Noncoding RNA: Biology, Chemistry, and Disease
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Piwi-piRNAによるトランスポゾンの転写抑制機構2017

    • Author(s)
      岩崎由香
    • Organizer
      NGS現場の会 第5回研究会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 生殖組織特異的に発現する小分子RNAによる 転移因子の発現制御2017

    • Author(s)
      岩崎由香
    • Organizer
      10th Symphony
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Essential function of Panoramix/Silencio-Nxf2 complex in the Drosophila Piwi-piRNA pathway2017

    • Author(s)
      Murano K , Iwasaki YW , Mashiko A, Siomi H
    • Organizer
      第19回日本RNA学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Modulation of chromatin accessibility by Piwi results in transcriptional regulation2016

    • Author(s)
      Iwasaki YW, Murano K, Ishizu H, Shibuya A, Iyoda Y, Siomi MC, Siomi H, Saito K
    • Organizer
      RNA2016: The 21st Annual Meeting of the RNA Society
    • Place of Presentation
      国立京都国際会館(京都)
    • Year and Date
      2016-06-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional analysis of CG9754/Panoramix/Silencio in the Drosophila Piwi-piRNA pathway2016

    • Author(s)
      Mashiko A, Iwasaki YW, Murano K, Siomi H
    • Organizer
      RNA2016: The 21st Annual Meeting of the RNA Society
    • Place of Presentation
      国立京都国際会館(京都)
    • Year and Date
      2016-06-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Modulation of Chromatin Accessibility by Piwi Results in Transcriptional Regulation2016

    • Author(s)
      Iwasaki YW, Murano K, Ishizu H, Shibuya A, Iyoda Y, Siomi MC, Siomi H, Saito K
    • Organizer
      RNA2016 The Joint Annual Meeting of the RNA Society and the RNA Society of Japan
    • Place of Presentation
      国立京都国際会館(京都府京都市)
    • Year and Date
      2016-06-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Piwi modulates chromatin accessibility for transcriptional regulation of transposons2016

    • Author(s)
      Iwasaki YW
    • Organizer
      International Symposium “Clues to Non-coding RNA Taxonomy”
    • Place of Presentation
      東京大学弥生講堂一条ホール(東京)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Piwi-piRNA complex regulates linker histone H1 for transcriptional silencing of their target transposons in the nucleus2015

    • Author(s)
      Iwasaki YW, Murano K, Ishizu H, Shibuya A, Iyoda Y, Siomi MC, Siomi H, Saito K
    • Organizer
      第38回日本分子生物学会年会 第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Piwi-piRNA promotes epigenetic silencing by regulating association of linker histone H1 with target transposon loci2015

    • Author(s)
      Iwasaki YW, Murano K, Ishizu H, Shibuya A, Iyoda Y, Siomi MC, Siomi H, Saito K
    • Organizer
      The 40th Naito Conference on “Epigenetics; From Histone Code to Therapeutic Strategy”
    • Place of Presentation
      シャトレーゼ ガトーキングタム(北海道札幌市)
    • Year and Date
      2015-09-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Piwi-piRNA regulates linker histone H1 for transcriptional silencing of their target transposable elements2015

    • Author(s)
      Iwasaki YW, Ishizu H, Shibuya A, Iyoda Y, Siomi MC, Siomi H, Saito K
    • Organizer
      日本RNA学会年会
    • Place of Presentation
      ホテルライフォート札幌(北海道札幌市)
    • Year and Date
      2015-07-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] Piwi-piRNA regulates association of linker histone H1 with target transposon loci in Drosophila2015

    • Author(s)
      Iwasaki YW, Ishizu H, Shibuya A, Iyoda Y, Siomi MC, Siomi H, Saito K
    • Organizer
      RNA2015 The Twentieth Annual Meeting of the RNA Society
    • Place of Presentation
      マディソン(アメリカ)
    • Year and Date
      2015-05-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2019-03-29  

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