| Budget Amount *help |
¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
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| Outline of Final Research Achievements |
Promyelocytic leukemia (PML) bodies are spherical nuclear structures of 0.1-.0 um in diameter, which are involved in various biological processes including cellular senescence, tumor suppression, and DNA replication and repair. To understand functional mechanism to regulate gene expression by PML, we developed a new technology called ALAP-seq, which allows us to profile genomic region interacting with nuclear bodies. We found that PML body associates with transcriptionally active gene loci and regulates their expression positively. Furthermore, some clustered genes on Y chromosome are coordinately regulated by PML body, which accompanied with epigenetic regulation including Histone modifications and DNA methylation. This study shed light into a novel mechanism to regulate clustered gene loci.
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