| Budget Amount *help |
¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
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| Outline of Final Research Achievements |
Crystal structures of cyanobacterium Photosystem II (PSII) in pH4 or pH9 condition were solved at 2.0-angstrom resolution. These acidic or basic PSII crystals changed from the neutral state were obtained by using the mixture solution of three kinds of buffers for the linear pH gradient without changing the composition of crystal mother liquid. Whole structures were no differences among neutral, acidic and basic structure. However, the pH-dependant structure changes were observed on the proton pathways from the active site of water-oxidation. The each of structural change was in the different locations between acidic and basic structure, respectively. We concluded that these parts were essential for proton transfer from the water-splitting reaction in PSII.
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