Elucidation of heterogeneous energy conversion mechanism of FoF1 ATP synthase

• Project/Area Number: 15H05591
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: The University of Tokyo
• Principal Investigator: Watanabe Rikiya 東京大学, 大学院工学系研究科(工学部), 講師 (30540108)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000); Fiscal Year 2016: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000); Fiscal Year 2015: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
• Keywords: 1分子生物物理学 / マイクロ分析装置 / F型ATP合成酵素 / 1分子生物物理 / 生物物理 / マイクロ・ナノデバイス

Outline of Final Research Achievements

In this study, we attempted to elucidate the detailed mechanism of heterogeneous energy conversion of FoF1 ATP synthase by developing novel analytical systems.
1, We established the platform to analyze aforementioned heterogeneous energy conversion by developing novel analytical technologies including artificial cell-membrane microsystems. 2, We elucidated the role of regulatory subunit, epsilon subunit, on heterogeneous energy conversion by single molecule manipulation using our original magnetic tweezers.

Research Products

• [Journal Article] Arrayed water-in-oil droplet bilayers for membrane transport analysis (2016)
  • Author(s): Watanabe, R., Soga, N., Hara, M., & Noji, H
  • Journal Title: Lab on a Chip Volume: NA Issue: 16 Pages: 3043-3048
  • DOI: 10.1039/c6lc00155f
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Single molecule analysis of membrane transporter activity by using a microsystem (2016)
  • Author(s): Watanabe, R., Soga, N., Ohdate, S., & Noji, H.
  • Journal Title: Methods in Molecular Biology Volume: NA
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Novel nano-device to measure voltage-driven membrane transporter activity (2016)
  • Author(s): Watanabe, R., Soga, N., & Noji, H.
  • Journal Title: IEEE Transactions on Nanotechnology Volume: 15 Issue: 1 Pages: 70-73
  • DOI: 10.1109/tnano.2015.2498167
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Attolitre-sized lipid bilayer chamber array for rapid detection of single transporters (2015)
  • Author(s): Soga, N., *Watanabe, R., & Noji, H.
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 11025-11025
  • DOI: 10.1038/srep11025
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Artificial biomembrane microsystems for highly sensitive analysis of membrane proteins【発表確定】 (2017)
  • Author(s): Watanabe, R.
  • Organizer: 253rd American Chemical Society National Meeting
  • Place of Presentation: アメリカ、サンフランシスコ
  • Int'l Joint Research / Invited
• [Presentation] 生体膜マイクロチップを利用したトランスポーターの高感度機能解析 (2017)
  • Author(s): 渡邉力也
  • Organizer: 第90回日本細菌学会総会
  • Place of Presentation: 日本、仙台
  • Invited
• [Presentation] マイクロチップが実現する膜輸送体の高感度構造機能解析 (2017)
  • Author(s): 渡邉力也
  • Organizer: 第97回日本化学会春季年会
  • Place of Presentation: 日本、横浜
  • Invited
• [Presentation] Single molecule analysis of membrane protein activities (2017)
  • Author(s): 渡邉力也
  • Organizer: 第64回日本応用物理学会春季学術講演会
  • Place of Presentation: 日本、横浜
  • Invited
• [Presentation] マイクロチップが実現する膜輸送体の高感度構造機能解析 (2016)
  • Author(s): 渡邉力也
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: 日本、横浜
  • Invited
• [Presentation] Artificial cell-membrane microsystems for highly sensitive analysis of membrane proteins (2016)
  • Author(s): Watanabe, R.
  • Organizer: Cold Spring Harbor Conferences Asia “Synthetic Biology”
  • Place of Presentation: 中国、蘇州
  • Int'l Joint Research / Invited
• [Presentation] Single molecule analysis of FoF1-ATP synthase (2016)
  • Author(s): 渡邉力也
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: 日本、筑波
  • Invited
• [Presentation] マイクロデバイスを利用した膜局在型回転分子モーターの1分子機能解析 (2016)
  • Author(s): 渡邉力也
  • Organizer: 第16回 日本蛋白質科学会年会
  • Place of Presentation: 日本、福岡
  • Invited
• [Presentation] Heterogeneous energy conversion mechanism of F1-ATPase (2015)
  • Author(s): Watanabe, R.
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 金沢大学、石川県、金沢市
  • Year and Date: 2015-09-14
  • Invited
• [Patent(Industrial Property Rights)] マイクロリアクタチップおよびその製造方法 (2017)
  • Inventor(s): 渡邉力也, 曽我直樹, 野地博行
  • Industrial Property Rights Holder: 渡邉力也, 曽我直樹, 野地博行
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-040664
  • Filing Date: 2017-03-03