| Budget Amount *help |
¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
Fiscal Year 2017: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2015: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
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| Outline of Final Research Achievements |
Plasma membrane potential is required for various cellular functions. The social amoebae Dictyostelium discoideum has been studied as a eukaryotic model organism to investigate chemotaxis, directed cell migration and differentiation. Chemotactic stimulation by cAMP is known to elicit cationic fluxes, involving proton and calcium ion, across the plasma membrane in Dictyostelium cells. This suggests that the membrane potential is also changed by cAMP stimulation. However it remains unknown how the membrane potential works in the eukaryotic signal transduction. In this study, we established the method to monitor the plasma membrane potential of Dictyostelium cells by fluorescence microscopy using a highly sensitive membrane potential dye. Then we found that the membrane potential of Dictyostelium cells was periodically changed depending on the cAMP signal relay in the aggregation stage. Results indicate that the membrane potential change regulate the cAMP signaling relay.
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