| Budget Amount *help |
¥22,750,000 (Direct Cost: ¥17,500,000、Indirect Cost: ¥5,250,000)
Fiscal Year 2017: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2016: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2015: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Outline of Final Research Achievements |
Since membrane proteins are large bimolecular systems, it is difficult to simulate their large conformational changes like gating of ion channel proteins by using conventional methods. To solve such problems, we developed new methods such as the multi-dimensional replica-exchange method, implicit solvent model, and parallel computing algorithm for cryo-EM flexible fitting. By using these methods, we simulated the P2X4 receptor, and elucidated the molecular mechanisms of the channel gating.
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