Roles and formation mechanism of organelles accumulation sites based on microtubule-endoplasmic reticulum interactions

• Project/Area Number: 15H05598
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: The University of Tokyo
• Principal Investigator: Hamada Takahiro 東京大学, 大学院総合文化研究科, 助教 (20452534)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000); Fiscal Year 2017: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2016: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000); Fiscal Year 2015: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
• Keywords: 微小管 / 小胞体 / アクチン繊維 / オルガネラ / RNA顆粒 / オルガネラ集積領域 / 植物 / メタボローム

Outline of Final Research Achievements

The study revealed microtubules and endoplasmic reticulum of organelles accumulation sites are important for amino acid metabolism and pentose phosphate metabolism in plants. The study also revealed that microtubules and actin filaments of organelles accumulation sites are important for stress granule formation and accumulation of small RNAs. The study also provided a new insight for the molecular mechanism of a microtubule associated protein, MOR1, in Arabidopsis.

Research Products

• [Journal Article] Isolation of Microtubules and Microtubule-Associated Proteins (2017)
  • Author(s): Hamada Takahiro、Sonobe Seiji
  • Journal Title: Methods in Molecular Biology Volume: 1511 Pages: 281-289
  • DOI: 10.1007/978-1-4939-6533-5_22
  • ISBN: 9781493965311, 9781493965335
• [Presentation] MOR1, a member of XMAP215/TOG family, modulates unique microtubule dynamics in Arabidopsis. (2018)
  • Author(s): Erin C. Kawazu, Tsuyoshi Fujimoto, Kentaro Tamura, Ikuko Hara-Nishimura, Yuichiro Watanabe, Takahiro Hamada
  • Organizer: 2018年生体運動合同班会議
• [Presentation] シロイヌナズナ微小管付随タンパク質MOR1の局在解析 (2017)
  • Author(s): 濱田隆宏、藤本剛史、田村謙太郎、西村いくこ、渡邊雄一郎
  • Organizer: 第５８回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島県鹿児島市）
  • Year and Date: 2017-03-16
• [Presentation] シロイヌナズナ微小管付随タンパク質MOR1の局在解析 (2017)
  • Author(s): 濱田隆宏、藤本剛史、田村謙太郎、西村いくこ、渡邊雄一郎
  • Organizer: 2017年生体運動合同班会議
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2017-01-06
• [Presentation] MOR1, a member of XMAP215/TOG family, modulates unique microtubule dynamics in Arabidopsis. (2017)
  • Author(s): Erin C. Kawazu, Tsuyoshi Fujimoto, Kentaro Tamura, Ikuko Hara-Nishimura, Yuichiro Watanabe, Takahiro Hamada
  • Organizer: Cold Spring Harbor Asia “Plant Cell and Developmental Biology”
  • Int'l Joint Research
• [Presentation] MOR1, a member of XMAP215/TOG family, modulates unique microtubule dynamics in Arabidopsis. (2017)
  • Author(s): Erin C. Kawazu, Tsuyoshi Fujimoto, Kentaro Tamura, Ikuko Hara-Nishimura, Yuichiro Watanabe, Takahiro Hamada
  • Organizer: 第６９回日本細胞生物学会大会
• [Presentation] 温度変化に応答した微小管ダイナミクス (2017)
  • Author(s): 濱田 隆宏
  • Organizer: 日本植物学会第81回大会
  • Invited
• [Presentation] オルガネラ集積領域における微小管の役割 (2016)
  • Author(s): 濱田隆宏
  • Organizer: 第５回 植物二次代謝フロンティア研究会
  • Place of Presentation: カリエンテ山口（山口県山口市）
  • Year and Date: 2016-11-05
  • Invited
• [Presentation] Microtubules mediate slow and short distance organelle transport in Arabidopsis (2016)
  • Author(s): Takahiro Hamada
  • Organizer: EMBL Symposium: Microtubules: From Atoms to Complex Systems
  • Place of Presentation: EMBL（ドイツ、ハイデルベルク）
  • Year and Date: 2016-05-29
  • Int'l Joint Research
• [Presentation] Microtubules mediate cytoplasmic metabolisms in Arabidopsis (2016)
  • Author(s): Takahiro Hamada
  • Organizer: 第５７回日本植物生理学会年会
  • Place of Presentation: 岩手大学（岩手県盛岡市）
  • Year and Date: 2016-03-18
  • Invited