• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Investigation of non-photochemical quenching mechanisms triggered by photosynthetic supercomplexes

Research Project

Project/Area Number 15H05599
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Plant molecular biology/Plant physiology
Research InstitutionNational Institute for Basic Biology

Principal Investigator

Tokutsu Ryutaro  基礎生物学研究所, 環境光生物学研究部門, 助教 (60613940)

Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2016: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2015: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywords環境応答 / 光防御 / 光合成 / 環境適応 / 光化学系 / 光化学系超複合体 / ストレス応答
Outline of Final Research Achievements

In this study, I investigated molecular mechanisms of the protective response against excess light (NPQ) in photosynthetic organisms. Using an unicellular green alga, I succeeded to isolate several photosynthetic supercomplexes, and revealed that those supercomplexes triggered different types of NPQ in the green alga. Considering together with the physiological researches of NPQ, these biochemical results suggest that photoprotective response in photosynthetic organisms is governed by several different molecular NPQ mechanisms.

Academic Significance and Societal Importance of the Research Achievements

自然界では時として光合成の許容量を上回る強度の光が降り注ぐ。このような過剰な光は、光合成生物の生存を脅かす危険なエネルギーとなり得る。このような危険なエネルギーを安全に処理するために、植物や藻類はNPQと呼ばれる光防御メカニズムを持っている。本研究では、単細胞緑藻を用いて光防御メカニズムを遺伝子・タンパク質レベルで解き明かした。この結果を利用することで、日照量・強度が過剰になりがちな環境においても効率的な光合成生産を維持可能な光合成生物(作物・燃料藻類)の創出が期待される。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (17 results)

All 2019 2018 2017 2016 2015 Other

All Journal Article (8 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 8 results,  Open Access: 6 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results) Remarks (3 results)

  • [Journal Article] Isolation of photoprotective signal transduction mutants by systematic bioluminescence screening in Chlamydomonas reinhardtii.2019

    • Author(s)
      Tokutsu R, Fujimura-Kamada K, Yamasaki T, Matsuo T, Minagawa J
    • Journal Title

      Sci Rep.

      Volume: 9(1) Issue: 1 Pages: 2820-2820

    • DOI

      10.1038/s41598-019-39785-z

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ten antenna proteins are associated with the core in the supramolecular organization of the photosystem I supercomplex in Chlamydomonas reinhardtii2019

    • Author(s)
      Kubota-Kawai Hisako、Burton-Smith Raymond N、Tokutsu Ryutaro、Song Chihong、Akimoto Seiji、Yokono Makio、Ueno Yoshifumi、Kim Eunchul、Watanabe Akimasa、Murata Kazuyoshi、Minagawa Jun
    • Journal Title

      Journal of Biological Chemistry

      Volume: 294 Issue: 12 Pages: 4304-4314

    • DOI

      10.1074/jbc.ra118.006536

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Investigation on the Thermodynamic Dissociation Kinetics of Photosystem II Supercomplexes To Determine the Binding Strengths of Light-Harvesting Complexes2018

    • Author(s)
      Kim Eunchul、Tokutsu Ryutaro、Minagawa Jun
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 122 Issue: 5 Pages: 1627-1630

    • DOI

      10.1021/acs.jpcb.7b12417

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] LHCSR1-dependent fluorescence quenching is mediated by excitation energy transfer from LHCII to photosystem I inChlamydomonas reinhardtii2018

    • Author(s)
      Kosuge Kotaro、Tokutsu Ryutaro、Kim Eunchul、Akimoto Seiji、Yokono Makio、Ueno Yoshifumi、Minagawa Jun
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: in press Issue: 14 Pages: 3722-3727

    • DOI

      10.1073/pnas.1720574115

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fluorescence lifetime analyses reveal how the high light-responsive protein LHCSR3 transforms PSII light-harvesting complexes into an energy-dissipative state2017

    • Author(s)
      Kim Eunchul、Akimoto Seiji、Tokutsu Ryutaro、Yokono Makio、Minagawa Jun
    • Journal Title

      Journal of Biological Chemistry

      Volume: 292 Issue: 46 Pages: 18951-18960

    • DOI

      10.1074/jbc.m117.805192

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A blue light photoreceptor mediates the feedback regulation of photosynthesis.2016

    • Author(s)
      Petroutsos D, Tokutsu R, Maruyama S, Flori S, Greiner A, Magneschi L, Cusant L, Kottke T, Mittag M, Hegemann P, Finazzi G and Minagawa J.
    • Journal Title

      Nature

      Volume: 537 Issue: 7621 Pages: 563-566

    • DOI

      10.1038/nature19358

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Chloroplast-mediated regulation of CO2-concentrating mechanism by Ca2+-binding protein CAS in the green alga Chlamydomonas reinhardtii2016

    • Author(s)
      L. Wang, T. Yamano, S. Takane, Y. Niikawa, C. Toyokawa, S. Ozawa, R. Tokutsu, Y. Takahashi, J. Minagawa, Y. Kanesaki, H. Yoshikawa, and H. Fukuzawa
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 113 Issue: 44 Pages: 12586-12591

    • DOI

      10.1073/pnas.1606519113

    • NAID

      120006425865

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Eyespot-dependent determination of the phototactic sign in Chlamydomonas reinhardtii2016

    • Author(s)
      Ueki, N., Ide, T., Mochiji, S., Kobayashi, Y., Tokutsu, R., Ohnishi, N., Yamaguchi, K., Shigenobu, S., Tanaka, K., Minagawa, J., Hisabori, T., Hirono, M., Wakabayashi, K.
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 印刷中 Issue: 19 Pages: 5266-5304

    • DOI

      10.1073/pnas.1525538113

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Phosphorylation of light harvesting complex II controls the spillover between photosystems2018

    • Author(s)
      Tokutsu Ryutaro、Kim Eunchul、Akimoto Seiji、Yokono Makio、Konomi Kamada、Norikazu Ohnishi、Akimasa Watanabe、Minagawa Jun
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 過剰な光を受け流す光合成超分子複合体2017

    • Author(s)
      得津隆太郎
    • Organizer
      第19回植物オルガネラワークショップ
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] The molecular functions of LHCII phosphorylation during state transitions2017

    • Author(s)
      Ryutaro Tokutsu, Eunchul Kim, Seiji Akimoto, Konomi Kamada, Norikazu Ohnishi, Jun Minagawa
    • Organizer
      第58回日本植物生理学会年会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 光合成タンパク質複合体のダイナミクスおよび環境応答の仕組み2015

    • Author(s)
      得津隆太郎
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      朱鷺メッセ:新潟コンベンションセンター・新潟県新潟市
    • Year and Date
      2015-09-06
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] The role of STT7 kinase during state transitions in Chlamydomonas reinhardtii2015

    • Author(s)
      Ryutaro Tokutsu and Jun Minagawa
    • Organizer
      Gordon Research Conference : Photosynthesis
    • Place of Presentation
      Bentley University in Waltham MA United States
    • Year and Date
      2015-06-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 多様な光環境における光合成タンパク質超複合体のダイナミクス2015

    • Author(s)
      得津隆太郎
    • Organizer
      第6回日本光合成学会年会
    • Place of Presentation
      岡山国際交流センター・岡山県岡山市
    • Year and Date
      2015-05-22
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Remarks] 研究成果論文が科学誌に受理されました!!(2018年3月)

    • URL

      http://chlamynibb.blogspot.jp/2018/03/20183.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 研究成果論文が科学誌に受理されました!!(2018年1月)

    • URL

      http://chlamynibb.blogspot.jp/2018/01/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 研究成果論文が科学誌に受理されました!!(2017年10月)

    • URL

      http://chlamynibb.blogspot.jp/2017/09/blog-post_29.html

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi