Investigation of non-photochemical quenching mechanisms triggered by photosynthetic supercomplexes

• Project/Area Number: 15H05599
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: Tokutsu Ryutaro 基礎生物学研究所, 環境光生物学研究部門, 助教 (60613940)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000); Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2017: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2016: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000); Fiscal Year 2015: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: 環境応答 / 光防御 / 光合成 / 環境適応 / 光化学系 / 光化学系超複合体 / ストレス応答

Outline of Final Research Achievements

In this study, I investigated molecular mechanisms of the protective response against excess light (NPQ) in photosynthetic organisms. Using an unicellular green alga, I succeeded to isolate several photosynthetic supercomplexes, and revealed that those supercomplexes triggered different types of NPQ in the green alga. Considering together with the physiological researches of NPQ, these biochemical results suggest that photoprotective response in photosynthetic organisms is governed by several different molecular NPQ mechanisms.

Academic Significance and Societal Importance of the Research Achievements

自然界では時として光合成の許容量を上回る強度の光が降り注ぐ。このような過剰な光は、光合成生物の生存を脅かす危険なエネルギーとなり得る。このような危険なエネルギーを安全に処理するために、植物や藻類はNPQと呼ばれる光防御メカニズムを持っている。本研究では、単細胞緑藻を用いて光防御メカニズムを遺伝子・タンパク質レベルで解き明かした。この結果を利用することで、日照量・強度が過剰になりがちな環境においても効率的な光合成生産を維持可能な光合成生物（作物・燃料藻類）の創出が期待される。

Research Products

• [Journal Article] Isolation of photoprotective signal transduction mutants by systematic bioluminescence screening in Chlamydomonas reinhardtii (2019)
  • Author(s): Tokutsu R, Fujimura-Kamada K, Yamasaki T, Matsuo T, Minagawa J
  • Journal Title: Scientific Reports Volume: 9(1) Pages: 2820-2820
  • DOI: 10.1038/s41598-019-39785-z
  • Peer Reviewed / Open Access
• [Journal Article] Ten antenna proteins are associated with the core in the supramolecular organization of the photosystem I supercomplex in Chlamydomonas reinhardtii (2019)
  • Author(s): Kubota-Kawai H., Burton-Smith R. N., Tokutsu R., Song C., Akimoto S., Yokono M., Ueno Y., Kim E., Watanabe A., Murata K., Minagawa J.
  • Journal Title: Journal of Biological Chemistry Volume: 294 Pages: 4304-4314
  • DOI: 10.1074/jbc.ra118.006536
  • Peer Reviewed
• [Journal Article] Investigation on the Thermodynamic Dissociation Kinetics of Photosystem II Supercomplexes to Determine the Binding Strengths of Light-Harvesting Complexes (2018)
  • Author(s): Kim Eunchul、Tokutsu Ryutaro、Minagawa Jun
  • Journal Title: Journal of Physical Chemistry B Volume: 122 Pages: 1627-1630
  • DOI: 10.1021/acs.jpcb.7b12417
  • Peer Reviewed / Open Access
• [Journal Article] LHCSR1-dependent fluorescence quenching is mediated by excitation energy transfer from LHCII to photosystem I in Chlamydomonas reinhardtii (2018)
  • Author(s): Kosuge Kotaro、Tokutsu Ryutaro、Kim Eunchul、Akimoto Seiji、Yokono Makio、Ueno Yoshifumi、Minagawa Jun
  • Journal Title: Proceedings of the National Academy of Sciences Volume: in press Pages: 3722-3727
  • DOI: 10.1073/pnas.1720574115
  • Peer Reviewed / Open Access
• [Journal Article] Fluorescence lifetime analyses reveal how the high light-responsive protein LHCSR3 transforms PSII light-harvesting complexes into an energy-dissipative state. (2017)
  • Author(s): Kim Eunchul、Akimoto Seiji、Tokutsu Ryutaro、Yokono Makio、Minagawa Jun
  • Journal Title: Journal of Biological Chemistry Volume: 292 Pages: 18951-18960
  • DOI: 10.1074/jbc.m117.805192
  • Peer Reviewed
• [Journal Article] A blue-light photoreceptor mediates the feedback regulation of photosynthesis (2016)
  • Author(s): Petroutsos D, Tokutsu R, Maruyama S, Flori S, Greiner A, Magneschi L, Cusant L, Kottke T, Mittag M, Hegemann P, Finazzi G and Minagawa J.
  • Journal Title: Nature Volume: 537 Pages: 563-566
  • DOI: 10.1038/nature19358
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Chloroplast-mediated regulation of CO2-concentrating mechanism by Ca2+-binding protein CAS in the green algaChlamydomonas reinhardtii (2016)
  • Author(s): L. Wang, T. Yamano, S. Takane, Y. Niikawa, C. Toyokawa, S. Ozawa, R. Tokutsu, Y. Takahashi, J. Minagawa, Y. Kanesaki, H. Yoshikawa, and H. Fukuzawa
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 113 Pages: 12586-12591
  • DOI: 10.1073/pnas.1606519113
  • NAID: 120006425865
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Eyespot-dependent determination of the phototactic sign inChlamydomonas reinhardtii (2016)
  • Author(s): Ueki, N., Ide, T., Mochiji, S., Kobayashi, Y., Tokutsu, R., Ohnishi, N., Yamaguchi, K., Shigenobu, S., Tanaka, K., Minagawa, J., Hisabori, T., Hirono, M., Wakabayashi, K.
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 印刷中 Pages: 5266-5304
  • DOI: 10.1073/pnas.1525538113
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Phosphorylation of light harvesting complex II controls the spillover between photosystems (2018)
  • Author(s): Tokutsu Ryutaro、Kim Eunchul、Akimoto Seiji、Yokono Makio、Konomi Kamada、Norikazu Ohnishi、Akimasa Watanabe、Minagawa Jun
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] 過剰な光を受け流す光合成超分子複合体 (2017)
  • Author(s): 得津隆太郎
  • Organizer: 第19回植物オルガネラワークショップ
  • Invited
• [Presentation] The molecular functions of LHCII phosphorylation during state transitions (2017)
  • Author(s): Ryutaro Tokutsu, Eunchul Kim, Seiji Akimoto, Konomi Kamada, Norikazu Ohnishi, Jun Minagawa
  • Organizer: 第58回日本植物生理学会年会
• [Presentation] 光合成タンパク質複合体のダイナミクスおよび環境応答の仕組み (2015)
  • Author(s): 得津隆太郎
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 朱鷺メッセ：新潟コンベンションセンター・新潟県新潟市
  • Year and Date: 2015-09-06
  • Invited
• [Presentation] The role of STT7 kinase during state transitions in Chlamydomonas reinhardtii (2015)
  • Author(s): Ryutaro Tokutsu and Jun Minagawa
  • Organizer: Gordon Research Conference : Photosynthesis
  • Place of Presentation: Bentley University in Waltham MA United States
  • Year and Date: 2015-06-28
  • Int'l Joint Research
• [Presentation] 多様な光環境における光合成タンパク質超複合体のダイナミクス (2015)
  • Author(s): 得津隆太郎
  • Organizer: 第6回日本光合成学会年会
  • Place of Presentation: 岡山国際交流センター・岡山県岡山市
  • Year and Date: 2015-05-22
  • Invited
• [Remarks] 研究成果論文が科学誌に受理されました！！(2018年3月)
  • URL: http://chlamynibb.blogspot.jp/2018/03/20183.html
• [Remarks] 研究成果論文が科学誌に受理されました！！(２０１８年１月)
  • URL: http://chlamynibb.blogspot.jp/2018/01/
• [Remarks] 研究成果論文が科学誌に受理されました！！(2017年10月)
  • URL: http://chlamynibb.blogspot.jp/2017/09/blog-post_29.html