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Exploration of subseafloor microbial biosphere through technical developments

Research Project

Project/Area Number 15H05608
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Ecology/Environment
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

MORONO Yuki  国立研究開発法人海洋研究開発機構, 高知コア研究所, グループリーダー代理 (30421845)

Research Collaborator ITO Motoo  
HOSHINO Tatsuhiko  
INAGAKI Fumio  
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Fiscal Year 2017: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2016: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2015: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Keywords海底下生命圏 / NanoSIMS / 安定同位体 / セルソーター / IODP
Outline of Final Research Achievements

In this study, we aimed to study microorganisms inhabiting subseafloor biosphere, which is one of the largest microbial biosphere on the Earth. Through developments of techniques to detect, separate, and selectively separate (sort) microbial cells with cleanest possible handling environment, we successfully detected very low biomass in the sediment and probe its metabolic activity through NanoSIMS (Nano-scale Secondary Ion Mass Spectroscopy) analysis.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (51 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (9 results) Journal Article (15 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 11 results,  Open Access: 7 results,  Acknowledgement Compliant: 7 results) Presentation (27 results) (of which Int'l Joint Research: 12 results,  Invited: 9 results)

  • [Int'l Joint Research] California Institute of Technology/Bigelow Laboratory for Ocean Sciences(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] ETH(スイス)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Aarhus University(デンマーク)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] 上海交通大学(中国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Aarhus大学(デンマーク)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] California Institute of Technology(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] カリフォルニア工科大学(米国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] ドイツ地球科学研究センター (GFZ)/ブレーメン大学(ドイツ)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] オーフス大学(デンマーク)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Assessment for capacity to capture aerosol-DNA by clean filters for molecular biology experiments2018

    • Author(s)
      Morono, Y., Hoshino, T., Terada, T., Suzuki, T., Sato, T., Yuasa, H., Kubota, Y., and Inagaki, F.
    • Journal Title

      Microbes and Environments

      Volume: in press

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Microbial diversity in sediments from the bottom of the Challenger Deep, Mariana Trench2018

    • Author(s)
      Nunoura, T., Nishizawa, M., Hirai, M., Shimamura, S., Harnvoravongchai, P., Koide, O., Morono, Y., Fukui, T., Inagaki, F., Miyazaki, J., Takaki, Y., and Takai, K.
    • Journal Title

      Microbes and Environments

      Volume: in press

    • NAID

      130007393737

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Bioturbation as a key driver behind the dominance of Bacteria over Archaea in near-surface sediment2017

    • Author(s)
      Chen, X., Andersen, T. J., Morono, Y., Inagaki, F., Jorgensen, B. B., and Lever. M. A.
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 2400-2400

    • DOI

      10.1038/s41598-017-02295-x

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Atribacteria from the Subseafloor Sedimentary Biosphere Disperse to the Hydrosphere through Submarine Mud Volcanoes2017

    • Author(s)
      Hoshino, T., Toki, T., Ijiri, A., Morono, Y., Machiyama, H., Ashi, J., Okamura, K., and Inagaki, F.
    • Journal Title

      Frontiers in Microbiology

      Volume: 8 Pages: 1135-1135

    • DOI

      10.3389/fmicb.2017.01135

    • NAID

      120006383302

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Methyl-compound use and slow growth characterize microbial life in 2-km-deep subseafloor coal and shale beds2017

    • Author(s)
      Trembath-Reichert, E., Morono, Y., Ijiri, A., Hoshino, T., Dowson, K. S., Inagaki, F., and Orphan, V. J.
    • Journal Title

      Proc. Natl. Acad. Sci. U.S.A.

      Volume: 114 Issue: 44

    • DOI

      10.1073/pnas.1707525114

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Aerobic and Anaerobic Methanotrophic Communities Associated with Methane Hydrates Exposed on the Seafloor: A High-Pressure Sampling and Stable Isotope-Incubation Experiment2017

    • Author(s)
      Case, D., Ijiri, A., Morono, Y., Tavormina, P., Orphan V. J., and Inagaki, F.
    • Journal Title

      Frontiers in Microbiology

      Volume: 8 Pages: 2569-2569

    • DOI

      10.3389/fmicb.2017.02569

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A modified SDS-based DNA extraction method for high quality environmental DNA from2016

    • Author(s)
      Vengadesh Perumal Natarajan, Xinxu Zhang, Yuki Morono, Fumio Inagaki and Fengping Wang
    • Journal Title

      Frontiers in Microbiology

      Volume: 7 Pages: 986-986

    • DOI

      10.3389/fmicb.2016.00986

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Dense microbial community on a ferromanganese nodule from the ultra-oligotrophic South Pacific Gyre: Implications for biogeochemical cycles2016

    • Author(s)
      Shiraishi F., Mitsunobu S., Suzuki K., Hoshino T., Morono Y., Inagaki F.
    • Journal Title

      Earth and Planetary Science Letters

      Volume: 447 Pages: 10-20

    • DOI

      10.1016/j.epsl.2016.04.021

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Size and Carbon Content of Sub-seafloor Microbial Cells at Landsort Deep, Baltic Sea2016

    • Author(s)
      Stefan Braun,, Yuki Morono, Sten Littmann, Marcel Kuypers, Husnu Aslan, Mingdong Dong, Bo B. Jorgensen, Bente Aa. Lomstein
    • Journal Title

      Frontiers in Microbiology

      Volume: 7 Pages: 1375-1375

    • DOI

      10.3389/fmicb.2016.01375

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Cellular content of biomolecules in sub-seafloor microbial communities2016

    • Author(s)
      Stefan Braun , Yuki Morono, Kevin W. Becker, Kai-Uwe Hinrichs, Kasper U. Kjeldsen, Bo B. Jorgensen, Bente Aa. Lomstein
    • Journal Title

      Geochimica et Cosmochimica Acta

      Volume: 188 Pages: 330-351

    • DOI

      10.1016/j.gca.2016.06.019

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Analysis of Low-Biomass Microbial Communities in the Deep Biosphere2016

    • Author(s)
      Yuki Morono and Fumio Inagaki
    • Journal Title

      Advances in Applied Microbiology

      Volume: 95 Pages: 149-178

    • DOI

      10.1016/bs.aambs.2016.04.001

    • ISBN
      9780128048023
    • Related Report
      2016 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] Osmium plasma coating for observation of microfossils, using optical and scanning electron microscopes2016

    • Author(s)
      TATSUHIKO YAMAGUCHI, Terada Takeshi,MORONO YUKI
    • Journal Title

      Paleontological Research

      Volume: -

    • NAID

      40020952394

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Exploring deep microbial life in coal-bearing sediments down to ~2.5km below the seafloor2015

    • Author(s)
      INAGAKI FUMIO, Kai-Uwe Hinrichs,KUBO YUSUKE,Marshall W. Bowles,Verena B. Heuer,Wei-Li Hong,HOSHINO TATSUHIKO,IJIRI AKIRA,IMACHI HIROYUKI,ITO MOTOO,KANEKO MASANORI,Mark A. Lever,Lin Yu-Shin,Barbara A. Methe,Morita Sumito,MORONO YUKI,et al.
    • Journal Title

      SCIENCE/AAAS/

      Volume: 349 Issue: 6246 Pages: 420-424

    • DOI

      10.1126/science.aaa6882

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Ecophysiology of Zetaproteobacteria Associated with Shallow Hydrothermal Iron-Oxyhydroxide Deposits in Nagahama Bay of Satsuma Iwo-Jima, Japan2015

    • Author(s)
      HOSHINO TATSUHIKO, Kuratomi Takashi,MORONO YUKI,Hori Tomoyuki,Oiwane Hisashi,Kiyokawa Shoichi,INAGAKI FUMIO
    • Journal Title

      Frontiers in Microbiology

      Volume: 6 Pages: 1554-1554

    • DOI

      10.3389/fmicb.2015.01554

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] NanoSIMSにより基質の流れを可視化するー未培養微生物のシングルセルレベルでの生理生態解析2015

    • Author(s)
      諸野 祐樹, 伊藤 元雄
    • Journal Title

      水環境学会誌

      Volume: 38 Pages: 375-379

    • Related Report
      2015 Annual Research Report
    • Acknowledgement Compliant
  • [Presentation] Biosphere in subseafloor environment and its ecophysiology2017

    • Author(s)
      諸野祐樹、寺田武志、星野辰彦、伊藤元雄、稲垣史生
    • Organizer
      日本細菌学会総会シンポジウム「Sleeping Microbes: 眠れる微生物の秘めた力」
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2017-03-20
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 海底下環境、および海底下生命圏を紐解く鍵となるSTXM分析2017

    • Author(s)
      諸野祐樹、浦本豪一郎、菅大暉、高橋嘉夫、寺田武志、伊藤元雄、稲垣史生
    • Organizer
      PF挿入光源ビームラインBL-19の戦略的利用に関する研究会
    • Place of Presentation
      高エネルギー加速器研究機構(茨城県つくば市)
    • Year and Date
      2017-01-26
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 海底下微生物の謎?~海底下に広がる世界~2017

    • Author(s)
      諸野 祐樹
    • Organizer
      高知大学理学部応用理学科災害科学コース特別講演
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] IODP Expedition 357: Atlantis Massif Serpentinization and Life2017

    • Author(s)
      Yuki Morono, Shino Suzuki, Motoo Ito, Goichiro Uramoto, Akira Ijiri, Gretchen Fruh-Green, Beth Orcutt, and IODP Expedition 357 Scientists
    • Organizer
      海洋リソスフェアの蛇紋岩化作用と物理・化学・生物プロセス- InterRidge-Japan 研究集会 -
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Excess fluid pressure development beneath the decollement at the Nankai subduction zone2017

    • Author(s)
      Takehiro HIROSE、Nana KAMIYA、Yuzuru Yamamoto、Heuer Verena、Fumio INAGAKI、Yuki Morono、 Kubo Yu'suke、Expedition 370 Scientists
    • Organizer
      JpGU-AGU 2017/SSS04-17
    • Related Report
      2017 Annual Research Report
  • [Presentation] 室戸沖デコルマゾーンおよび沈み込む堆積物の変形と物性の側方バリエーション:Exp. 370速報2017

    • Author(s)
      山本 由弦、Stephen Bowden、藤内 智士、Kiho Yang、Man-Yin Tsang、廣瀬 丈洋、神谷 奈々、奥津 なつみ、山本 裕二、稲垣 史生、Verena Heuer、諸野 祐樹、久保 雄介、Expedition 370 Scientists
    • Organizer
      日本地球惑星科学連合大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] IODP Expedition 370: Temperature Limit of the Deep Biosphere off Muroto2017

    • Author(s)
      Yuki Morono, Verena B. Heuer, Fumio Inagaki, Yusuke Kubo, Lena Maeda, and the Expedition 370 Scientists
    • Organizer
      日本地球惑星科学連合大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The global survey of subseafloor sedimentary microbiomes2017

    • Author(s)
      TATSUHIKO HOSHINO, HIDEYUKI DOI, LARS WORMER, YUKI MORONO, STEVEN D’HONDT, KAI-UWE HINRICHS, FUMIO INAGAKI
    • Organizer
      Goldschmidt2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 室戸沖海底下掘削による生命生息温度限界への挑戦(T-リミット)2017

    • Author(s)
      諸野 祐樹, Hauer B. Verena, 稲垣 史生, 久保 雄介, 前田 玲奈, Expedition 370 Scientists
    • Organizer
      環境微生物系合同大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Global microbial diversity in subseafloor sediment2017

    • Author(s)
      Tatsuhiko Hoshino, Hideyuki Doi, Lars Wormer, Yuki Morono, Kai-Uwe Hinrichs, Fumio Inagaki Global microbial diversity in subseafloor sediment
    • Organizer
      The international society for subsurface microbiology 2017 conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Temperature Limit of the Deep Subseafloor Biosphere in the Nankai Trough Subduction Zone off Cape Muroto (IODP T-Limit Expedition 370)2017

    • Author(s)
      Yuki Morono, Verena B. Heuer, Fumio Inagaki, Yusuke Kubo, Lena Maeda and The Expedition 370 Scientists
    • Organizer
      AGU Fall Meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] オープンクリーンシステムを用いた実験環境下におけるDNA汚染の影響評価2017

    • Author(s)
      寺田武志、諸野祐樹、星野辰彦、鈴木剛人、佐藤卓広、湯浅久史、久保田裕仁、稲垣史生
    • Organizer
      環境微生物系合同大会2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] Distribution of aerobic microbial activities in ultra-oligotrophic sediments of the South Pacific Gyre2016

    • Author(s)
      Yuki Morono, Takeshi Terada, Motoo Ito, Tatsuhiko Hoshino, Steven D'Hondt, Fumio Inagaki
    • Organizer
      16th International Symposium on Microbial Ecology
    • Place of Presentation
      カナダ、モントリオール
    • Year and Date
      2016-08-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Distribution of Aerobic Microbial Activities in Ultra-Oligotrophic Sediments of the South Pacific Gyre2016

    • Author(s)
      YUKI MORONO, TAKESHI TERADA, MOTOO ITO, TATSUHIKO HOSHINO, STEVEN D'HONDT, FUMIO INAGAKI
    • Organizer
      Goldschmidt 2016
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-06-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] NanoSIMS Reveals Activity in Deepest Samples Ever Collected by Marine Scientific Drilling2016

    • Author(s)
      ELIZABETH TREMBATH-REICHERT, YUKI MORONO, AKIRA IJIRI, KATHERINE S. DAWSON, KAI-UWE HINRICHS, FUMIO INAGAKI , VICTORIA J. ORPHAN
    • Organizer
      Goldschmidt 2016
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-06-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Technological breakthroughs in search of the deep subseafloor biosphere2016

    • Author(s)
      諸野 祐樹、寺田 武志、伊藤 元雄、稲垣 史生
    • Organizer
      Japan Geoscience Union Meeting 2016
    • Place of Presentation
      幕張メッセ(千葉県千葉市)
    • Year and Date
      2016-05-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 海底下に広がる巨大生命圏とその探索ー最先端技術で生命の実態にせまるー2016

    • Author(s)
      諸野祐樹
    • Organizer
      日本農芸化学会中四国支部創立15周年記念第22回若手研究者シンポジウム
    • Place of Presentation
      高知大学農学部(高知県南国市)
    • Year and Date
      2016-04-23
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 海底着底型掘削装置による北大西洋アトランティス岩体掘削2016

    • Author(s)
      Gretchen Fruh-Green, Beth Orcutt, Yuki Morono, The IODP Expedition 357 Scientists
    • Organizer
      第8回深海底表層地盤の研究ワークショップ
    • Place of Presentation
      海洋研究開発機構横浜研究所(神奈川県横浜市)
    • Year and Date
      2016-04-08
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] OXIC AND ANOXIC REGIONS OF SUBSEAFLOOR SEDIMENT2015

    • Author(s)
      D'Hondt Steven, Pockalny Robert,Spivack Arthur,INAGAKI FUMIO,Murray RICHARD,Adhikari Rishi Ram,Britta Gribsholt,Kallmeyer Jens,McKinley Claire,MORONO YUKI,Roy Hans,Justine Sauvage,Wiebke Ziebis
    • Organizer
      2015 AGU Fall Meeting
    • Place of Presentation
      Moscone center (USA)
    • Year and Date
      2015-12-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Size and Carbon Content of Sub-seafloor Microbial Cells2015

    • Author(s)
      Stefan Braun, MORONO YUKI,Sten Littmann,Jorgensen Bo Barker,Lomstein Bente Aa.
    • Organizer
      AGU Fall Meeting 2015
    • Place of Presentation
      Moscone center (San Francisco, USA)
    • Year and Date
      2015-12-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of biomolecules of sub-seafloor microbial cells separated from the sediment matrix2015

    • Author(s)
      Braun Stefan, MORONO YUKI,Kevin Becker,Hinrichs Kai-Uwe,Jorgensen Bo Barker,Lomstein Bente Aa.
    • Organizer
      AGU Fall Meeting 2015
    • Place of Presentation
      Moscone Center (San Francisco, USA)
    • Year and Date
      2015-12-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 海底下生命のセンサス:海底下微生物群集の空間的分布と地球環境との相互作用の解明2015

    • Author(s)
      星野 辰彦, 諸野 祐樹,稲垣 史生
    • Organizer
      第16回極限環境生物学会
    • Place of Presentation
      東京海洋大学 品川キャンパス (東京都港区)
    • Year and Date
      2015-11-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] Technical developments to explore sedimentary biosphere: current status, limitation, and future perspectives2015

    • Author(s)
      MORONO YUKI,
    • Organizer
      CDEBI Sediment Workshop
    • Place of Presentation
      University of Southern California (USA)
    • Year and Date
      2015-06-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 超貧栄養海域のマンガン団塊表面に密集する微生物群集2015

    • Author(s)
      白石 史人, 光延 聖,諸野 祐樹,鈴木 勝彦,稲垣 史生
    • Organizer
      日本地球惑星科学連合2015年大会
    • Place of Presentation
      幕張メッセ (千葉県千葉市)
    • Year and Date
      2015-05-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 薩摩硫黄島の浅海性熱水マウンドにおける鉄酸化微生物群集の分子生態学的・鉱物学的特徴2015

    • Author(s)
      星野 辰彦, 倉富 隆,堀 知行,諸野 祐樹,稲垣 史生,清川 晶一
    • Organizer
      日本地球惑星科学連合2015年大会
    • Place of Presentation
      幕張メッセ (千葉県千葉市)
    • Year and Date
      2015-05-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 掘削コア試料の高品質、長期間保管を可能とする磁場振動型凍結とその評価2015

    • Author(s)
      諸野 祐樹, 寺田 武志,山本 裕二,肖 楠,廣瀬 丈洋,菅野 正也,大和田 哲男,稲垣 史生
    • Organizer
      日本地球惑星科学連合2015年大会
    • Place of Presentation
      幕張メッセ (千葉県千葉市)
    • Year and Date
      2015-05-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] Probing metabolic activity of aerobic microbial cells in ultraoligotrophic sediments of the South Pacific Gyre2015

    • Author(s)
      MORONO YUKI, Terada Takeshi,ITO MOTOO,INAGAKI FUMIO
    • Organizer
      Deep Carbon Observatory International Science Meeting
    • Place of Presentation
      Lisbon (Portugal)
    • Year and Date
      2015-05-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited

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Published: 2015-04-16   Modified: 2022-10-14  

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