Exploration of subseafloor microbial biosphere through technical developments

• Project/Area Number: 15H05608
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Ecology/Environment
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: MORONO Yuki 国立研究開発法人海洋研究開発機構, 高知コア研究所, グループリーダー代理 (30421845)
• Research Collaborator: ITO Motoo ; HOSHINO Tatsuhiko ; INAGAKI Fumio
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000); Fiscal Year 2017: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000); Fiscal Year 2016: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000); Fiscal Year 2015: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
• Keywords: 海底下生命圏 / NanoSIMS / 安定同位体 / セルソーター / IODP

Outline of Final Research Achievements

In this study, we aimed to study microorganisms inhabiting subseafloor biosphere, which is one of the largest microbial biosphere on the Earth. Through developments of techniques to detect, separate, and selectively separate (sort) microbial cells with cleanest possible handling environment, we successfully detected very low biomass in the sediment and probe its metabolic activity through NanoSIMS (Nano-scale Secondary Ion Mass Spectroscopy) analysis.

Research Products

• [Int'l Joint Research] California Institute of Technology/Bigelow Laboratory for Ocean Sciences(米国)
• [Int'l Joint Research] ETH(スイス)
• [Int'l Joint Research] Aarhus University(デンマーク)
• [Int'l Joint Research] 上海交通大学(中国)
• [Int'l Joint Research] Aarhus大学(デンマーク)
• [Int'l Joint Research] California Institute of Technology(米国)
• [Int'l Joint Research] カリフォルニア工科大学(米国)
• [Int'l Joint Research] ドイツ地球科学研究センター （GFZ）/ブレーメン大学(ドイツ)
• [Int'l Joint Research] オーフス大学(デンマーク)
• [Journal Article] Assessment for capacity to capture aerosol-DNA by clean filters for molecular biology experiments (2018)
  • Author(s): Morono, Y., Hoshino, T., Terada, T., Suzuki, T., Sato, T., Yuasa, H., Kubota, Y., and Inagaki, F.
  • Journal Title: Microbes and Environments Volume: in press
• [Journal Article] Microbial diversity in sediments from the bottom of the Challenger Deep, Mariana Trench (2018)
  • Author(s): Nunoura, T., Nishizawa, M., Hirai, M., Shimamura, S., Harnvoravongchai, P., Koide, O., Morono, Y., Fukui, T., Inagaki, F., Miyazaki, J., Takaki, Y., and Takai, K.
  • Journal Title: Microbes and Environments Volume: in press
  • NAID: 130007393737
• [Journal Article] Bioturbation as a key driver behind the dominance of Bacteria over Archaea in near-surface sediment (2017)
  • Author(s): Chen, X., Andersen, T. J., Morono, Y., Inagaki, F., Jorgensen, B. B., and Lever. M. A.
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 2400-2400
  • DOI: 10.1038/s41598-017-02295-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Atribacteria from the Subseafloor Sedimentary Biosphere Disperse to the Hydrosphere through Submarine Mud Volcanoes (2017)
  • Author(s): Hoshino, T., Toki, T., Ijiri, A., Morono, Y., Machiyama, H., Ashi, J., Okamura, K., and Inagaki, F.
  • Journal Title: Frontiers in Microbiology Volume: 8 Pages: 1135-1135
  • DOI: 10.3389/fmicb.2017.01135
  • NAID: 120006383302
  • Peer Reviewed / Open Access
• [Journal Article] Methyl-compound use and slow growth characterize microbial life in 2-km-deep subseafloor coal and shale beds (2017)
  • Author(s): Trembath-Reichert, E., Morono, Y., Ijiri, A., Hoshino, T., Dowson, K. S., Inagaki, F., and Orphan, V. J.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. Volume: 114 Issue: 44
  • DOI: 10.1073/pnas.1707525114
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Aerobic and Anaerobic Methanotrophic Communities Associated with Methane Hydrates Exposed on the Seafloor: A High-Pressure Sampling and Stable Isotope-Incubation Experiment (2017)
  • Author(s): Case, D., Ijiri, A., Morono, Y., Tavormina, P., Orphan V. J., and Inagaki, F.
  • Journal Title: Frontiers in Microbiology Volume: 8 Pages: 2569-2569
  • DOI: 10.3389/fmicb.2017.02569
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A modified SDS-based DNA extraction method for high quality environmental DNA from (2016)
  • Author(s): Vengadesh Perumal Natarajan, Xinxu Zhang, Yuki Morono, Fumio Inagaki and Fengping Wang
  • Journal Title: Frontiers in Microbiology Volume: 7 Pages: 986-986
  • DOI: 10.3389/fmicb.2016.00986
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Dense microbial community on a ferromanganese nodule from the ultra-oligotrophic South Pacific Gyre: Implications for biogeochemical cycles (2016)
  • Author(s): Shiraishi F., Mitsunobu S., Suzuki K., Hoshino T., Morono Y., Inagaki F.
  • Journal Title: Earth and Planetary Science Letters Volume: 447 Pages: 10-20
  • DOI: 10.1016/j.epsl.2016.04.021
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Size and Carbon Content of Sub-seafloor Microbial Cells at Landsort Deep, Baltic Sea (2016)
  • Author(s): Stefan Braun,, Yuki Morono, Sten Littmann, Marcel Kuypers, Husnu Aslan, Mingdong Dong, Bo B. Jorgensen, Bente Aa. Lomstein
  • Journal Title: Frontiers in Microbiology Volume: 7 Pages: 1375-1375
  • DOI: 10.3389/fmicb.2016.01375
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Cellular content of biomolecules in sub-seafloor microbial communities (2016)
  • Author(s): Stefan Braun , Yuki Morono, Kevin W. Becker, Kai-Uwe Hinrichs, Kasper U. Kjeldsen, Bo B. Jorgensen, Bente Aa. Lomstein
  • Journal Title: Geochimica et Cosmochimica Acta Volume: 188 Pages: 330-351
  • DOI: 10.1016/j.gca.2016.06.019
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Analysis of Low-Biomass Microbial Communities in the Deep Biosphere (2016)
  • Author(s): Yuki Morono and Fumio Inagaki
  • Journal Title: Advances in Applied Microbiology Volume: 95 Pages: 149-178
  • DOI: 10.1016/bs.aambs.2016.04.001
  • ISBN: 9780128048023
  • Acknowledgement Compliant
• [Journal Article] Osmium plasma coating for observation of microfossils, using optical and scanning electron microscopes (2016)
  • Author(s): TATSUHIKO YAMAGUCHI, Terada Takeshi,MORONO YUKI
  • Journal Title: Paleontological Research Volume: -
  • NAID: 40020952394
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Exploring deep microbial life in coal-bearing sediments down to ~2.5km below the seafloor (2015)
  • Author(s): INAGAKI FUMIO, Kai-Uwe Hinrichs,KUBO YUSUKE,Marshall W. Bowles,Verena B. Heuer,Wei-Li Hong,HOSHINO TATSUHIKO,IJIRI AKIRA,IMACHI HIROYUKI,ITO MOTOO,KANEKO MASANORI,Mark A. Lever,Lin Yu-Shin,Barbara A. Methe,Morita Sumito,MORONO YUKI,et al.
  • Journal Title: SCIENCE/AAAS/ Volume: 349 Issue: 6246 Pages: 420-424
  • DOI: 10.1126/science.aaa6882
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Ecophysiology of Zetaproteobacteria Associated with Shallow Hydrothermal Iron-Oxyhydroxide Deposits in Nagahama Bay of Satsuma Iwo-Jima, Japan (2015)
  • Author(s): HOSHINO TATSUHIKO, Kuratomi Takashi,MORONO YUKI,Hori Tomoyuki,Oiwane Hisashi,Kiyokawa Shoichi,INAGAKI FUMIO
  • Journal Title: Frontiers in Microbiology Volume: 6 Pages: 1554-1554
  • DOI: 10.3389/fmicb.2015.01554
  • Peer Reviewed / Open Access
• [Journal Article] NanoSIMSにより基質の流れを可視化するー未培養微生物のシングルセルレベルでの生理生態解析 (2015)
  • Author(s): 諸野 祐樹, 伊藤 元雄
  • Journal Title: 水環境学会誌 Volume: 38 Pages: 375-379
  • Acknowledgement Compliant
• [Presentation] Biosphere in subseafloor environment and its ecophysiology (2017)
  • Author(s): 諸野祐樹、寺田武志、星野辰彦、伊藤元雄、稲垣史生
  • Organizer: 日本細菌学会総会シンポジウム「Sleeping Microbes: 眠れる微生物の秘めた力」
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2017-03-20
  • Invited
• [Presentation] 海底下環境、および海底下生命圏を紐解く鍵となるSTXM分析 (2017)
  • Author(s): 諸野祐樹、浦本豪一郎、菅大暉、高橋嘉夫、寺田武志、伊藤元雄、稲垣史生
  • Organizer: PF挿入光源ビームラインBL－19の戦略的利用に関する研究会
  • Place of Presentation: 高エネルギー加速器研究機構（茨城県つくば市）
  • Year and Date: 2017-01-26
  • Invited
• [Presentation] 海底下微生物の謎？～海底下に広がる世界～ (2017)
  • Author(s): 諸野 祐樹
  • Organizer: 高知大学理学部応用理学科災害科学コース特別講演
  • Invited
• [Presentation] IODP Expedition 357: Atlantis Massif Serpentinization and Life (2017)
  • Author(s): Yuki Morono, Shino Suzuki, Motoo Ito, Goichiro Uramoto, Akira Ijiri, Gretchen Fruh-Green, Beth Orcutt, and IODP Expedition 357 Scientists
  • Organizer: 海洋リソスフェアの蛇紋岩化作用と物理・化学・生物プロセス- InterRidge-Japan 研究集会 -
  • Invited
• [Presentation] Excess fluid pressure development beneath the decollement at the Nankai subduction zone (2017)
  • Author(s): Takehiro HIROSE、Nana KAMIYA、Yuzuru Yamamoto、Heuer Verena、Fumio INAGAKI、Yuki Morono、 Kubo Yu'suke、Expedition 370 Scientists
  • Organizer: JpGU-AGU 2017/SSS04-17
• [Presentation] 室戸沖デコルマゾーンおよび沈み込む堆積物の変形と物性の側方バリエーション：Exp. 370速報 (2017)
  • Author(s): 山本 由弦、Stephen Bowden、藤内 智士、Kiho Yang、Man-Yin Tsang、廣瀬 丈洋、神谷 奈々、奥津 なつみ、山本 裕二、稲垣 史生、Verena Heuer、諸野 祐樹、久保 雄介、Expedition 370 Scientists
  • Organizer: 日本地球惑星科学連合大会
• [Presentation] IODP Expedition 370: Temperature Limit of the Deep Biosphere off Muroto (2017)
  • Author(s): Yuki Morono, Verena B. Heuer, Fumio Inagaki, Yusuke Kubo, Lena Maeda, and the Expedition 370 Scientists
  • Organizer: 日本地球惑星科学連合大会
• [Presentation] The global survey of subseafloor sedimentary microbiomes (2017)
  • Author(s): TATSUHIKO HOSHINO, HIDEYUKI DOI, LARS WORMER, YUKI MORONO, STEVEN D’HONDT, KAI-UWE HINRICHS, FUMIO INAGAKI
  • Organizer: Goldschmidt2017
  • Int'l Joint Research
• [Presentation] 室戸沖海底下掘削による生命生息温度限界への挑戦（T-リミット） (2017)
  • Author(s): 諸野 祐樹, Hauer B. Verena, 稲垣 史生, 久保 雄介, 前田 玲奈, Expedition 370 Scientists
  • Organizer: 環境微生物系合同大会
• [Presentation] Global microbial diversity in subseafloor sediment (2017)
  • Author(s): Tatsuhiko Hoshino, Hideyuki Doi, Lars Wormer, Yuki Morono, Kai-Uwe Hinrichs, Fumio Inagaki Global microbial diversity in subseafloor sediment
  • Organizer: The international society for subsurface microbiology 2017 conference
  • Int'l Joint Research
• [Presentation] Temperature Limit of the Deep Subseafloor Biosphere in the Nankai Trough Subduction Zone off Cape Muroto (IODP T-Limit Expedition 370) (2017)
  • Author(s): Yuki Morono, Verena B. Heuer, Fumio Inagaki, Yusuke Kubo, Lena Maeda and The Expedition 370 Scientists
  • Organizer: AGU Fall Meeting
  • Int'l Joint Research
• [Presentation] オープンクリーンシステムを用いた実験環境下におけるDNA汚染の影響評価 (2017)
  • Author(s): 寺田武志、諸野祐樹、星野辰彦、鈴木剛人、佐藤卓広、湯浅久史、久保田裕仁、稲垣史生
  • Organizer: 環境微生物系合同大会2017
• [Presentation] Distribution of aerobic microbial activities in ultra-oligotrophic sediments of the South Pacific Gyre (2016)
  • Author(s): Yuki Morono, Takeshi Terada, Motoo Ito, Tatsuhiko Hoshino, Steven D'Hondt, Fumio Inagaki
  • Organizer: 16th International Symposium on Microbial Ecology
  • Place of Presentation: カナダ、モントリオール
  • Year and Date: 2016-08-22
  • Int'l Joint Research
• [Presentation] Distribution of Aerobic Microbial Activities in Ultra-Oligotrophic Sediments of the South Pacific Gyre (2016)
  • Author(s): YUKI MORONO, TAKESHI TERADA, MOTOO ITO, TATSUHIKO HOSHINO, STEVEN D'HONDT, FUMIO INAGAKI
  • Organizer: Goldschmidt 2016
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-06-27
  • Int'l Joint Research
• [Presentation] NanoSIMS Reveals Activity in Deepest Samples Ever Collected by Marine Scientific Drilling (2016)
  • Author(s): ELIZABETH TREMBATH-REICHERT, YUKI MORONO, AKIRA IJIRI, KATHERINE S. DAWSON, KAI-UWE HINRICHS, FUMIO INAGAKI , VICTORIA J. ORPHAN
  • Organizer: Goldschmidt 2016
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-06-27
  • Int'l Joint Research
• [Presentation] Technological breakthroughs in search of the deep subseafloor biosphere (2016)
  • Author(s): 諸野 祐樹、寺田 武志、伊藤 元雄、稲垣 史生
  • Organizer: Japan Geoscience Union Meeting 2016
  • Place of Presentation: 幕張メッセ（千葉県千葉市）
  • Year and Date: 2016-05-22
  • Int'l Joint Research / Invited
• [Presentation] 海底下に広がる巨大生命圏とその探索ー最先端技術で生命の実態にせまるー (2016)
  • Author(s): 諸野祐樹
  • Organizer: 日本農芸化学会中四国支部創立15周年記念第22回若手研究者シンポジウム
  • Place of Presentation: 高知大学農学部（高知県南国市）
  • Year and Date: 2016-04-23
  • Invited
• [Presentation] 海底着底型掘削装置による北大西洋アトランティス岩体掘削 (2016)
  • Author(s): Gretchen Fruh-Green, Beth Orcutt, Yuki Morono, The IODP Expedition 357 Scientists
  • Organizer: 第8回深海底表層地盤の研究ワークショップ
  • Place of Presentation: 海洋研究開発機構横浜研究所（神奈川県横浜市）
  • Year and Date: 2016-04-08
  • Invited
• [Presentation] OXIC AND ANOXIC REGIONS OF SUBSEAFLOOR SEDIMENT (2015)
  • Author(s): D'Hondt Steven, Pockalny Robert,Spivack Arthur,INAGAKI FUMIO,Murray RICHARD,Adhikari Rishi Ram,Britta Gribsholt,Kallmeyer Jens,McKinley Claire,MORONO YUKI,Roy Hans,Justine Sauvage,Wiebke Ziebis
  • Organizer: 2015 AGU Fall Meeting
  • Place of Presentation: Moscone center (USA)
  • Year and Date: 2015-12-17
  • Int'l Joint Research
• [Presentation] Size and Carbon Content of Sub-seafloor Microbial Cells (2015)
  • Author(s): Stefan Braun, MORONO YUKI,Sten Littmann,Jorgensen Bo Barker,Lomstein Bente Aa.
  • Organizer: AGU Fall Meeting 2015
  • Place of Presentation: Moscone center (San Francisco, USA)
  • Year and Date: 2015-12-17
  • Int'l Joint Research
• [Presentation] Analysis of biomolecules of sub-seafloor microbial cells separated from the sediment matrix (2015)
  • Author(s): Braun Stefan, MORONO YUKI,Kevin Becker,Hinrichs Kai-Uwe,Jorgensen Bo Barker,Lomstein Bente Aa.
  • Organizer: AGU Fall Meeting 2015
  • Place of Presentation: Moscone Center (San Francisco, USA)
  • Year and Date: 2015-12-17
  • Int'l Joint Research
• [Presentation] 海底下生命のセンサス：海底下微生物群集の空間的分布と地球環境との相互作用の解明 (2015)
  • Author(s): 星野 辰彦, 諸野 祐樹,稲垣 史生
  • Organizer: 第16回極限環境生物学会
  • Place of Presentation: 東京海洋大学 品川キャンパス （東京都港区）
  • Year and Date: 2015-11-07
• [Presentation] Technical developments to explore sedimentary biosphere: current status, limitation, and future perspectives (2015)
  • Author(s): MORONO YUKI,
  • Organizer: CDEBI Sediment Workshop
  • Place of Presentation: University of Southern California (USA)
  • Year and Date: 2015-06-25
  • Int'l Joint Research / Invited
• [Presentation] 超貧栄養海域のマンガン団塊表面に密集する微生物群集 (2015)
  • Author(s): 白石 史人, 光延 聖,諸野 祐樹,鈴木 勝彦,稲垣 史生
  • Organizer: 日本地球惑星科学連合2015年大会
  • Place of Presentation: 幕張メッセ (千葉県千葉市）
  • Year and Date: 2015-05-26
• [Presentation] 薩摩硫黄島の浅海性熱水マウンドにおける鉄酸化微生物群集の分子生態学的・鉱物学的特徴 (2015)
  • Author(s): 星野 辰彦, 倉富 隆,堀 知行,諸野 祐樹,稲垣 史生,清川 晶一
  • Organizer: 日本地球惑星科学連合2015年大会
  • Place of Presentation: 幕張メッセ (千葉県千葉市）
  • Year and Date: 2015-05-26
• [Presentation] 掘削コア試料の高品質、長期間保管を可能とする磁場振動型凍結とその評価 (2015)
  • Author(s): 諸野 祐樹, 寺田 武志,山本 裕二,肖 楠,廣瀬 丈洋,菅野 正也,大和田 哲男,稲垣 史生
  • Organizer: 日本地球惑星科学連合2015年大会
  • Place of Presentation: 幕張メッセ (千葉県千葉市）
  • Year and Date: 2015-05-24
• [Presentation] Probing metabolic activity of aerobic microbial cells in ultraoligotrophic sediments of the South Pacific Gyre (2015)
  • Author(s): MORONO YUKI, Terada Takeshi,ITO MOTOO,INAGAKI FUMIO
  • Organizer: Deep Carbon Observatory International Science Meeting
  • Place of Presentation: Lisbon (Portugal)
  • Year and Date: 2015-05-08
  • Int'l Joint Research / Invited