| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2018: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
Plants utilize nitrate not only as a nitrogen source but also as a signaling molecule that induces the expression of genes for nitrate acquisition and utilization. We had previously shown that that nitrate signals induce the gene expression by activating the NLP transcription factors. In this study, we studied this mechanism in detail. We found that nitrate signals activate calcium ion signaling pathway, which in turn leads to the phosphorylation of NLP proteins. The phosphorylated form of NLP proteins is the active form, and activates the gene expression. We also found that the interaction among NLP proteins through their carboxy-terminal PB1 domain is a requisite for the full induction of target genes.
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