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Identification of molecular mechanisms of plant iron sensing

Research Project

Project/Area Number 15H05617
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Plant nutrition/Soil science
Research InstitutionIshikawa Prefectural University

Principal Investigator

Kobayashi Takanori  石川県立大学, 生物資源環境学部, 准教授 (70590206)

Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2017: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Keywords鉄欠乏 / イネ / 遺伝子発現 / 転写因子 / ユビキチンリガーゼ / センサー / センシング / 植物 / 遺伝子 / シグナル伝達 / ユビキチン
Outline of Final Research Achievements

In order to clarify intracellular iron sensors in plants, we investigated molecular mechanisms linking metal binding and regulation of iron deficiency responses in rice transcription factor IDEF1 and ubiquitin ligase HRZs. We did not observe clear changes in DNA binding property and protein stability of IDEF1 by iron nutritional conditions. In contrast, our results suggested changes in protein stability, localization and function of HRZs by their metal binding. Proteins involved in iron-sulfur cluster biosynthesis and transport, and a bZIP transcription factor, were suggested to function downstream of HRZs. Jasmonate signaling was found to be involved in early responses to iron deficiency and HRZ-based regulation of iron homeostasis in rice.

Academic Significance and Societal Importance of the Research Achievements

世界の耕地の30%以上を占める石灰質土壌では、植物は鉄を吸収しにくく生育に適さないが、本研究はこのような土壌でも良好に育つ植物の開発につながる。さらに、植物が土壌中から吸収する鉄は、食物の鉄分の由来となるため、本研究は鉄栄養価の高い米などの食物の開発につながる。学術的には、植物の鉄感知を担う細胞内鉄センサーと鉄欠乏シグナルの実体は未だに明らかになっていない。本研究はこれらの解明につながる重要な知見を提供するものである。

Report

(4 results)
  • 2018 Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (21 results)

All 2018 2017 2016 2015 Other

All Journal Article (4 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (16 results) (of which Int'l Joint Research: 5 results,  Invited: 6 results) Remarks (1 results)

  • [Journal Article] Rice HRZ ubiquitin ligases are crucial for response to excess iron2018

    • Author(s)
      Aung MS, Kobayashi T, Masuda H, Nishizawa NK
    • Journal Title

      Physiol. Plant.

      Volume: in press Issue: 3 Pages: 282-296

    • DOI

      10.1111/ppl.12698

    • NAID

      120006942648

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 植物、鉄とユビキチンリガーゼ2017

    • Author(s)
      小林高範
    • Journal Title

      ユビキチンネオバイオロジー ニュースレター. 新学術領域「ユビキチンネオバイオロジー」

      Volume: -- Pages: 7-11

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Jasmonate signaling is activated in the very early stages of iron deficiency responses in rice roots.2016

    • Author(s)
      Kobayashi, T. (corresponding author), Itai, R.N., Senoura, T., Oikawa, T., Ishimaru, Y., Ueda, M., Nakanishi, H., Nishizawa, N.K.
    • Journal Title

      Plant Molecular Biology

      Volume: 印刷中 Issue: 4-5 Pages: 533-547

    • DOI

      10.1007/s11103-016-0486-3

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Regulating Subcellular Metal Homeostasis: The Key to Crop Improvement.2016

    • Author(s)
      Khurram Bashir, Sultana Rasheed, Takanori Kobayashi, Motoaki Seki, Naoko K. Nishizawa.
    • Journal Title

      Frontiers in Plant Science

      Volume: 7 Pages: 1192-1192

    • DOI

      10.3389/fpls.2016.01192

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Regulation of iron deficiency responses by HRZ ubiquitin ligases and their interacting partners2018

    • Author(s)
      Takanori Kobayashi
    • Organizer
      19th International Symposium on Iron Nutrition and Interactions in Plants (ISINIP)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 植物の鉄欠乏応答と鉄感知2018

    • Author(s)
      小林高範
    • Organizer
      日本農芸化学会2018年度大会 シンポジウム講演
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] HRZ 結合性グルタレドキシンによるイネの鉄欠乏応答制御2018

    • Author(s)
      小林高範、西澤直子
    • Organizer
      日本土壌肥料学会2018年度神奈川大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] イネのグルタレドキシンはユビキチンリガーゼ HRZ とともに鉄欠乏応答を制御する2018

    • Author(s)
      小林高範、西澤直子
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 鉄結合性ユビキチンリガーゼ HRZ による鉄栄養制御2018

    • Author(s)
      小林高範、メイサンアウン、増田寛志、瀬野浦武志、西澤直子
    • Organizer
      第11回北陸合同バイオシンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 植物の鉄欠乏応答解明による植物の生産性とミネラル栄養価の向上2017

    • Author(s)
      小林高範
    • Organizer
      平成29年度石川県立大学シーズ発表会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 遺伝子組み換えと育種で鉄分豊富なコメを作る2017

    • Author(s)
      小林高範
    • Organizer
      平成29年度第1回石川県食品技術研究者ネットワークオープンセミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Rice HRZ ubiquitin ligases are involved in both iron deficiency and excess responses and jasmonate signaling2017

    • Author(s)
      Takanori Kobayashi, May Sann Aung, Reiko Nakanishi Itai, Takeshi Senoura, Hiroshi Masuda, Takaya Oikawa, Yasuhiro Ishimaru, Minoru Ueda, Hiromi Nakanishi, Naoko K. Nishizawa
    • Organizer
      XVIII International Plant Nutrition Colloquium (XVIIIth IPNC)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] HRZ ubiquitin ligases negatively regulate rice iron uptake, transport and accumulation under various iron conditions2017

    • Author(s)
      Takanori Kobayashi, May Sann Aung, Reiko Nakanishi Itai, Takeshi Senoura, Hiroshi Masuda, Takaya Oikawa, Yasuhiro Ishimaru, Minoru Ueda, Hiromi Nakanishi, Naoko K. Nishizawa
    • Organizer
      ASPB annual meeting in 2017 (Plant Biology 2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] イネのユビキチンリガーゼHRZは鉄過剰耐性に関与する2017

    • Author(s)
      メイサンアウン、小林高範、増田寛志、西澤直子
    • Organizer
      日本土壌肥料学会 2017年度仙台大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] HRZ ubiquitin ligases and jasmonate signaling regulate iron deficiency responses in rice roots.2016

    • Author(s)
      Takanori Kobayashi, Reiko Nakanishi Itai, Takeshi Senoura, Takaya Oikawa, Yasuhiro Ishimaru, Minoru Ueda, Hiromi Nakanishi, Naoko K. Nishizawa
    • Organizer
      18th International Symposium on Iron Nutrition and Interactions in Plants
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regulation of rice iron deficiency response and possible iron sensing by HRZ ubiquitin ligases.2016

    • Author(s)
      Takanori Kobayashi
    • Organizer
      International Workshop on Soil-Microbe-Plant Interaction
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ユビキチンリガーゼ HRZ による植物の鉄栄養制御と鉄感知2016

    • Author(s)
      小林高範
    • Organizer
      第40回日本鉄バイオサイエンス学会学術集会
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 少ない鉄でもよく育ち、鉄を効率的に貯めるイネ2016

    • Author(s)
      小林高範
    • Organizer
      平成28年度石川県立大学シーズ発表会
    • Related Report
      2016 Annual Research Report
  • [Presentation] イネ鉄欠乏応答の初期にジャスモン酸経路が活性化される2016

    • Author(s)
      小林高範・板井玲子・瀬野浦武志・及川貴也・石丸泰寛・上田実・中西啓仁・西澤直子
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学(盛岡)
    • Related Report
      2015 Annual Research Report
  • [Presentation] イネ鉄欠乏応答におけるユビキチンリガーゼHRZとジャスモン酸経路の関与2015

    • Author(s)
      小林高範・板井玲子・瀬野浦武志・及川貴也・石丸泰寛・上田実・中西啓仁・西澤直子
    • Organizer
      日本土壌肥料学会2015年度京都大会
    • Place of Presentation
      京都大学(京都)
    • Related Report
      2015 Annual Research Report
  • [Remarks] 石川県立大学 生物資源工学研究所 植物細胞工学研究室

    • URL

      http://ribb.ishikawa-pu.ac.jp/pct/index.htm

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report

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Published: 2015-04-16   Modified: 2020-03-30  

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