| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2017: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Outline of Final Research Achievements |
In order to clarify intracellular iron sensors in plants, we investigated molecular mechanisms linking metal binding and regulation of iron deficiency responses in rice transcription factor IDEF1 and ubiquitin ligase HRZs. We did not observe clear changes in DNA binding property and protein stability of IDEF1 by iron nutritional conditions. In contrast, our results suggested changes in protein stability, localization and function of HRZs by their metal binding. Proteins involved in iron-sulfur cluster biosynthesis and transport, and a bZIP transcription factor, were suggested to function downstream of HRZs. Jasmonate signaling was found to be involved in early responses to iron deficiency and HRZ-based regulation of iron homeostasis in rice.
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