The actual host range of plasmids in microbial consortia
| Project/Area Number |
15H05618
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Shizuoka University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥24,570,000 (Direct Cost: ¥18,900,000、Indirect Cost: ¥5,670,000)
Fiscal Year 2017: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2016: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2015: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
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| Keywords | プラスミド / 宿主 / 嫌気性細菌 / 複合微生物系 / 接合伝達 / GFP |
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| Outline of Final Research Achievements |
Behaviors of plasmids under aerobic and anaerobic conditions is important to understand bacterial evolution and adaptation mechanism. Exogenous plasmid capturing was performed by triparental matings, and self-transmissible plasmids were successfully obtained from model environmental samples. They included an incompatibility group (Inc) P-1 plasmid, four Inc PromA plasmids and one novel plasmid. Comparisons of conjugation frequency with the captured plasmids and IncP-7 plasmid under different concentration of oxygens showed that the oxygen were not required for conjugation. The conjugation frequency was lower under low concentration of oxygen, while the effect of oxygen changed between different hosts. Based on the mating assays of each donor of IncP-1 and Inc PromA plasmids with model microbial consortia, the host range of plasmids could change by the concentration of oxygen.
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Report
(4 results)
Research Products
(36 results)
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[Journal Article] Comparisons of the transferability of plasmids pCAR1, pB10, R388, and NAH7 among Pseudomonas putida at different cell densities2016
Author(s)
Kosuke Yanagida, Ayako Sakuda, Chiho Suzuki-Minakuchi, Masaki Shintani, Kazuhiro Matsui, Kazunori Okada, and Hideaki Nojiri
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 印刷中
Issue: 5
Pages: 1020-1023
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] Behaviors of plasmids in soil microcosms2017
Author(s)
Shintani M, Nour EH, Elsayed T, Blau K, Bziuk N, Jechalke S, Sproer C, Bunk B, Overmann J, Smalla K,
Organizer
6th International Symposium on Biosorption and Biodegradation/Bioremediation
Related Report
Int'l Joint Research / Invited
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