| Budget Amount *help |
¥24,570,000 (Direct Cost: ¥18,900,000、Indirect Cost: ¥5,670,000)
Fiscal Year 2017: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2016: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2015: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
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| Outline of Final Research Achievements |
Behaviors of plasmids under aerobic and anaerobic conditions is important to understand bacterial evolution and adaptation mechanism. Exogenous plasmid capturing was performed by triparental matings, and self-transmissible plasmids were successfully obtained from model environmental samples. They included an incompatibility group (Inc) P-1 plasmid, four Inc PromA plasmids and one novel plasmid. Comparisons of conjugation frequency with the captured plasmids and IncP-7 plasmid under different concentration of oxygens showed that the oxygen were not required for conjugation. The conjugation frequency was lower under low concentration of oxygen, while the effect of oxygen changed between different hosts. Based on the mating assays of each donor of IncP-1 and Inc PromA plasmids with model microbial consortia, the host range of plasmids could change by the concentration of oxygen.
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