| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2018: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2016: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Outline of Final Research Achievements |
The occurrence of ultra-small filterable bacteria capable of passing through 0.2-micrometer-pore-size filters has been reported recently. In this study, we determined the growth characteristics, physiological and biochemical properties, and genomic structures of two representative filterable strains (novel alphaproteobacterium IZ6 and Oligoflexus tunisiensis Shr3), isolated from terrestrial environments. As a result, functional genes involved in nitrogen cycle, such as nitrogen fixation and denitrification, were detected from the genome information, and the nitrogen fixation ability was confirmed by a culture experiment using the novel strain IZ6. Furthermore, metagenomic analysis of filterable bacterial populations present in environmental samples revealed the existence of novel metabolic pathways in uncultured lineages. Thus, it was disclosed that microorganisms with novel biological functions exist in the neglected filterable fraction.
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