Selective regulation of salicylic acid signaling for inducing the systemic acquired resistance of various crops

• Project/Area Number: 15H05621
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Applied biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: Miyakawa Takuya 東京大学, 大学院農学生命科学研究科(農学部), 特任准教授 (50596559)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000); Fiscal Year 2017: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000); Fiscal Year 2016: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000); Fiscal Year 2015: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
• Keywords: 植物ホルモン / サリチル酸 / 抵抗性誘導剤 / X線結晶構造解析

Outline of Final Research Achievements

Salicylic acid (SA) is a plant hormone that regulates the immune response of plants and induces systemic acquired resistance (SAR) to prevent infection of plant pathogens. In SA signaling, NPR proteins function as SA receptors and control the expression of SA responsive genes. In this study, we investigated the complex formation of NPR proteins and the transcription factor for SA responsive genes, and then conducted a structural biology study on the regulation mechanism of SA signaling by NPR proteins.

Research Products

• [Journal Article] Crystal structure of a Ca2+-dependent regulator of flagellar motility reveals the open-closed structural transition (2018)
  • Author(s): Tomoki Shojima, Feng Hou, Yusuke Takahashi, Yoshitaka Matsumura, Masahiko Okai, Akira Nakamura, Katsutoshi Mizuno, Kazuo Inaba, Masaki Kojima, Takuya Miyakawa & Masaru Tanokura
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 2014-2014
  • DOI: 10.1038/s41598-018-19898-7
  • NAID: 120007134496
  • Peer Reviewed / Open Access
• [Journal Article] BC-Box Motif-Mediated Neuronal Differentiation of Somatic Stem Cells. (2018)
  • Author(s): Kanno H, Xu Y, Miyakawa T, Kubo A, Higashida T, Kobayashi NB, Yoshida T, Tanokura M
  • Journal Title: International Journal of Molecular Science Volume: 19 Issue: 2 Pages: 466-466
  • DOI: 10.3390/ijms19020466
  • Peer Reviewed / Open Access
• [Journal Article] Studies on the regulatory mechanism of isocitrate dehydrogenase 2 using acetylation mimics (2017)
  • Author(s): Xu Y, Liu L, Nakamura A, Someya S, Miyakawa T, Tanokura M
  • Journal Title: Sci Rep Volume: 7 Issue: 1 Pages: 9785-9785
  • DOI: 10.1038/s41598-017-10337-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Laminarinase from Flavobacterium sp. reveals the structural basis of thermostability and substrate specificity (2017)
  • Author(s): Qin HM, Miyakawa T, Inoue A, Nakamura A, Nishiyama R, Ojima T, Tanokura M
  • Journal Title: Sci Rep Volume: 7 Issue: 1 Pages: 11425-11425
  • DOI: 10.1038/s41598-017-11542-0
  • Peer Reviewed / Open Access
• [Journal Article] Overview of the mechanism of cytoskeletal motors based on structure (2017)
  • Author(s): Kato Y, Miyakawa T, Tanokura M
  • Journal Title: Biophys Rev Volume: 10 Issue: 2 Pages: 571-581
  • DOI: 10.1007/s12551-017-0368-1
  • Peer Reviewed
• [Journal Article] Structural basis for the regulation of phytohormone receptors (2017)
  • Author(s): Takuya Miyakawa, Masaru Tanokura
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: -
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Structural basis of unique ligand specificity of KAI2-like protein from parasitic weed Striga hermonthica (2016)
  • Author(s): Yuqun Xu, Takuya Miyakawa, Hidemitsu Nakamura, Akira Nakamura, Yusaku Imamura, Tadao Asami, Masaru Tanokura
  • Journal Title: Sci. Rep. Volume: 6 Issue: 1 Pages: 31386-31386
  • DOI: 10.1038/srep31386
  • Peer Reviewed / Open Access
• [Presentation] ブラシノステロイド情報伝達におけるマスター転写因子のDNA結合特異性の構造基盤 (2018)
  • Author(s): 野﨑翔平、宮川拓也、浅見忠男、中野雄司、田之倉 優
  • Organizer: 日本農芸化学会2018年度大会
• [Presentation] アセチル化模倣型変異体を用いたIDH2の活性調節機構の構造学的解析 (2018)
  • Author(s): 徐玉群、宮川拓也、劉凌ウェン、中村顕、染谷慎一、田之倉 優
  • Organizer: 日本農芸化学会2018年度大会
• [Presentation] 植物のストレス応答・生長制御に関する構造生物学的研究 (2015)
  • Author(s): 宮川拓也
  • Organizer: 日本農芸化学会 2015年度 第1回 関東支部例会
  • Place of Presentation: 東京
  • Year and Date: 2015-06-20
  • Invited