Mechanisms of anhydrobiosis-associated gene regulation in the cells and tissues of chironomid Polypedilum vanderplanki.
| Project/Area Number |
15H05622
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Gusev Oleg 国立研究開発法人理化学研究所, 科学技術ハブ推進本部, マネージャー (30711999)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000)
Fiscal Year 2017: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2016: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2015: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Keywords | anhydrobiosis / CAGE / desiccation / chironomid / transcriptomics / cis-elements / tissue-specific / genome / transcriptome / non-coding RNA / PIMT / LEA proteins / HSFT / 遺伝子発現 / 乾燥耐性 / Pv11 / bi-directional promoters |
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| Outline of Final Research Achievements |
The project was aimed to uncover tissue-specific genetic mechanisms of desiccation tolerance in anhydrobiotic midge Polypedilum vanderplanki by combination of several omics techniques. We defined a number of non-coding RNA , transcribed from multi-copy conservative regions of the genome, and those with the features of enhancer RNA (i.e. non-coding RNA transcribed from enhancers in the genome) with expression patterns correlated with that of anhydrobiosis-involved genes (for examples the genes located in ARIds region of the genome.
We found that genetic mechanism of tissue specificity and broad expression of protective genes across tissues is the strategy of anhydrobiosis and can be used for biomimetics. Next, using CAGE data, we demonstrated that, heat shock transcription factor (HSF1) is one of the key regulators of transcriptional activation in response to dehydration in the cells of the anhydrobiotic chironomid. We further confirmed it by RNAi experiments.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] A new anhydrobiotic midge from Malawi, Polypedilum pembai sp. nov. (Diptera: Chironomidae), closely related to the desiccation tolerant midge, Polypedilum vanderplanki Hinton.2017
Author(s)
3.Cornette R, Yamamoto N, Yamamoto M, Kobayashi T, Petrova NA, Gusev O, Shimura S, Kikawada T, Pemba D, Okuda T
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Journal Title
Systematic Entomology
Volume: 42
Issue: 4
Pages: 814-825
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] A new anhydrobiotic midge from Malawi, Polypedilum pembai sp. nov. (Diptera: Chironomidae), closely related to the desiccation tolerant midge, Polypedilum vanderplanki Hinton2017
Author(s)
Richard Cornette, Nao Yamamoto, Masaru Yamamoto, Tadashi Kobayashi, Ninel A. Petrova5, Oleg Gusev, Sachiko Shimura, Takahiro Kikawada, Dylo Pemba And Takashi Okuda
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Journal Title
Systematic Entomology
Volume: accepted
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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