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Post-translational regulation of auxin biosynthesis in Arabidopsis

Research Project

Project/Area Number 15H05623
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Bioorganic chemistry
Research InstitutionTohoku University

Principal Investigator

Mashiguchi Kiyoshi  東北大学, 生命科学研究科, 助教 (00569725)

Research Collaborator KASAHARA hiroyuki  東京農工大学, 教授
Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2017: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2016: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2015: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywords植物ホルモン / オーキシン / フラビン含有モノオキシゲナーゼ / 生合成
Outline of Final Research Achievements

Auxins, a class of plant hormones, are essential for plant growth and development. The natural auxin indole-3-acetic acid (IAA) is biosynthesized from tryptophan via indole-3-pyruvic acid by TAA aminotransferase and YUCCA flavin-containing monooxygenase families. Although much progresses in the transcriptional regulation of IAA biosynthetic genes has been made in recent years, its post-translational regulation has remained unclear. In this study, we identified and analyzed a YUCCA-interacting protein to elucidate the regulation mechanism of IAA biosynthesis in Arabidopsis. Our results suggest that the regulation of YUCCAs at the level of protein has a role in the homeostatic regulation of IAA.

Academic Significance and Societal Importance of the Research Achievements

植物ホルモンとして働くオーキシンの生合成における鍵酵素タンパク質であるYUCCA(ユッカ)ファミリーが、遺伝子レベルだけではなく、タンパク質レベルの調節によっても制御されている可能性が示されました。今回モデル植物のシロイヌナズナから見出したYUCCAの調節タンパク質のホモログ(よく似たタンパク質)は他の双子葉植物にも存在するので、本メカニズムは様々な植物において共通したものかもしれません。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (20 results)

All 2019 2018 2017 2016 Other

All Journal Article (5 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (12 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Agrobacterium tumefaciens Enhances Biosynthesis of Two Distinct Auxins in the Formation of Crown Galls2019

    • Author(s)
      Mashiguchi Kiyoshi、Hisano Hiroshi、Takeda-Kamiya Noriko、Takebayashi Yumiko、Ariizumi Tohru、Gao Yangbin、Ezura Hiroshi、Sato Kazuhiro、Zhao Yunde、Hayashi Ken-ichiro、Kasahara Hiroyuki
    • Journal Title

      Plant and Cell Physiology

      Volume: 60 Issue: 1 Pages: 29-37

    • DOI

      10.1093/pcp/pcy182

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Structural Basis of Karrikin and Non-natural Strigolactone Perception in Physcomitrella patens2019

    • Author(s)
      Burger Marco、Mashiguchi Kiyoshi、Lee Hyun Jee、Nakano Misaki、Takemoto Kodai、Seto Yoshiya、Yamaguchi Shinjiro、Chory Joanne
    • Journal Title

      Cell Reports

      Volume: 26 Issue: 4 Pages: 855-865.e5

    • DOI

      10.1016/j.celrep.2019.01.003

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] An allelic series at the KARRIKIN INSENSITIVE 2 locus of Arabidopsis thaliana decouples ligand hydrolysis and receptor degradation from downstream signalling2018

    • Author(s)
      Yao Jiaren、Mashiguchi Kiyoshi、Scaffidi Adrian、Akatsu Tomoki、Melville Kim T.、Morita Ryo、Morimoto Yu、Smith Steven M.、Seto Yoshiya、Flematti Gavin R.、Yamaguchi Shinjiro、Waters Mark T.
    • Journal Title

      The Plant Journal

      Volume: 96 Issue: 1 Pages: 75-89

    • DOI

      10.1111/tpj.14017

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Yucasin DF, a potent and persistent inhibitor of auxin biosynthesis in plants2017

    • Author(s)
      Tsugafune Shinichi、Mashiguchi Kiyoshi、Fukui Kosuke、Takebayashi Yumiko、Nishimura Takeshi、Sakai Tatsuya、Shimada Yukihisa、Kasahara Hiroyuki、Koshiba Tomokazu、Hayashi Ken-ichiro
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 13992-13992

    • DOI

      10.1038/s41598-017-14332-w

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Involvement of STH7 in light-adapted development in Arabidopsis thaliana promoted by both strigolactone and karrikin.2017

    • Author(s)
      Thussagunpanit J, Nagai Y, Nagae M, Mashiguchi K, Mitsuda N, Ohme-Takagi M, Nakano T, Nakamura H, Asami T.
    • Journal Title

      Biosci Biotechnol Biochem.

      Volume: 81 Issue: 2 Pages: 292-301

    • DOI

      10.1080/09168451.2016.1254536

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] シロイヌナズナの葉の老化におけるストリゴラクトンの機能解析2018

    • Author(s)
      横山千晃, 東大野むつみ, 森本優, 瀬戸義哉, 秋山康紀, 増口潔, 山口信次郎
    • Organizer
      植物化学調節学会第53回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] DWARF14-LIKEによるストリゴラクトン生合成の制御機構の解析2018

    • Author(s)
      森田諒, 田中海, 亀岡啓, 経塚淳子, 瀬戸義哉, 増口潔, 山口信次郎
    • Organizer
      植物化学調節学会第53回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ストリゴラクトン非依存的な信号伝達を可能とする変異型AtD14の改変2018

    • Author(s)
      平野伶奈, 櫻田茜, 瀬戸義哉, 増口潔, 山口信次郎
    • Organizer
      植物化学調節学会第53回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] シロイヌナズナにおけるストリゴラクトン輸送体の探索2018

    • Author(s)
      鈴木馨、来生貴也、森愛実、秋山康紀、瀬戸義哉、増口潔、山口信次郎
    • Organizer
      第59回日本植物生理学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Regulation of strigolactone biosynthesis by the D14 like pathway in rice2017

    • Author(s)
      増口潔
    • Organizer
      2nd International Congress on Strigolactones
    • Place of Presentation
      トリノ(イタリア)
    • Year and Date
      2017-03-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Strigolactone transport in Arabidopsis2017

    • Author(s)
      Mashiguchi Kiyoshi
    • Organizer
      Plant Biology at Tohoku University
    • Related Report
      2017 Annual Research Report
  • [Presentation] エポキシ型ジベレリンの分析法の確立2017

    • Author(s)
      石田俊晃、札野翔子、Zhang Y、Zhu H、Zhang S、He Z、瀬戸義哉、増口潔、山口信次郎
    • Organizer
      植物化学調節学会第52回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ストリゴラクトンの新規生合成阻害剤の解析2017

    • Author(s)
      岩崎愛、石田俊晃、櫻田茜、田中海、瀬戸義哉、笠原博幸、増口潔、山口信次郎
    • Organizer
      植物化学調節学会第52回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シロイヌナズナのDWARF14 によるストリゴラクトンの受容と加水分解の経時的な解析2017

    • Author(s)
      安井令、瀬戸義哉、来生貴也、秋山康紀、増口潔、山口信次郎
    • Organizer
      植物化学調節学会第52回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シロイヌナズナにおけるストリゴラクトン輸送体の探索2017

    • Author(s)
      鈴木馨、来生貴也、森愛美、秋山康紀、瀬戸義哉、増口潔、山口信次郎
    • Organizer
      植物化学調節学会第52回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Strigolactone biosynthesis and its regulation2016

    • Author(s)
      増口潔
    • Organizer
      Frontiers in parasitic plant and host interactions
    • Place of Presentation
      奈良先端科学技術大学院大学(奈良県)
    • Year and Date
      2016-08-30
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Identification of essential amino acid residues in KAI2/HTL in Arabidopsis.2016

    • Author(s)
      増口潔
    • Organizer
      The 22nd International Conference on Plant Growth Substances (IPGSA)
    • Place of Presentation
      トロント(カナダ)
    • Year and Date
      2016-06-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Book] 第11章 ストリゴラクトン. 新しい植物ホルモンの科学・第3版2016

    • Author(s)
      増口潔, 山口信次郎
    • Publisher
      講談社
    • Related Report
      2016 Annual Research Report
  • [Remarks] 東北大学生命科学研究科・活性分子動態分野

    • URL

      http://labo.lifesci.tohoku.ac.jp/biomol/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 東北大学生命科学研究科活性分子動態分野

    • URL

      http://labo.lifesci.tohoku.ac.jp/biomol/

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2020-03-30  

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