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Genetic bases of bacteria for symbiotic association with an insect

Research Project

Project/Area Number 15H05638
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Insect science
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Kikuchi Yoshitomo  国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (30571864)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000)
Fiscal Year 2017: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2016: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2015: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Keywords共生 / 細菌 / 昆虫 / 進化 / 遺伝的基盤 / 共生細菌 / 微生物 / 遺伝子
Outline of Final Research Achievements

The bean bug Riptortus pedestris is associated with Burkholderia symbionts in its gut crypts. In order to understand how the symbiotic bacteria stably colonize the midgut crypts and which services they provide to the host, the cytology, physiology and transcriptomics of free-living and gut-colonizing symbionts were compared. The analyses revealed that midgut-colonizing bacteria were smaller in size and had increased stress sensitivity, lost motility, and an altered cell surface. Transcriptomics revealed that metabolic pathways of sulfur compounds and the assimilation of insect nitrogen wastes were up-regulated in the gut-colonizing symbionts. The data also suggested that the in vivo symbionts produced all essential amino acids and B vitamins. Together, these findings suggest that the symbiont recycles host metabolic wastes, and in return, the bacterial symbiont provides the host with essential nutrients, contributing to the rapid growth and enhanced reproduction of the bean bug host.

Academic Significance and Societal Importance of the Research Achievements

細菌との共生は多くの動植物に見られる普遍的な現象であるが、その遺伝的基盤についてはほとんど解明されていない。本研究ではホソヘリカメムシの共生細菌(Burkholderia)を対象に、共生細菌の機能や共生のメカニズムを解明する目的で研究を行い、本共生細菌が宿主昆虫体内で遺伝子発現を大きく変化させ、宿主の代謝老廃物を活発に利用することで昆虫体内環境に高度に適応していることが明らかとなった。本研究成果は共生細菌の昆虫体内適応機構を遺伝子レベルで解明した点で学術的に重要である。加えて、ホソヘリカメムシは大豆の重要害虫であることから、共生細菌をターゲットにした新たな防除法の開発にも繋がる可能性がある。

Report

(4 results)
  • 2018 Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (46 results)

All 2019 2018 2017 2016 2015 Other

All Int'l Joint Research (5 results) Journal Article (16 results) (of which Int'l Joint Research: 9 results,  Peer Reviewed: 16 results,  Open Access: 6 results,  Acknowledgement Compliant: 1 results) Presentation (23 results) (of which Int'l Joint Research: 7 results,  Invited: 8 results) Remarks (2 results)

  • [Int'l Joint Research] CNRS(フランス)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Ghent University(ベルギー)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] フランス国立科学研究センター(CNRS)(フランス)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] CNRS(フランス)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] 釜山大学(韓国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Comparative cytology, physiology and transcriptomics of Burkholderia insecticola in symbiosis with the bean bug Riptortus pedestris and in culture2019

    • Author(s)
      Ohbayashi Tsubasa、Futahashi Ryo、Terashima Mia、Barri?re Quentin、Lamouche Florian、Takeshita Kazutaka、Meng Xian-Ying、Mitani Yasuo、Sone Teruo、Shigenobu Shuji、Fukatsu Takema、Mergaert Peter、Kikuchi Yoshitomo
    • Journal Title

      The ISME Journal

      Volume: 印刷中 Issue: 6 Pages: 1469-1483

    • DOI

      10.1038/s41396-019-0361-8

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Identification of active and taxonomically diverse 1,4-dioxane degraders in a full-scale activated sludge system by high-sensitivity stable isotope probing2018

    • Author(s)
      Aoyagi Tomo、Morishita Fumiaki、Sugiyama Yutaka、Ichikawa Daisuke、Mayumi Daisuke、Kikuchi Yoshitomo、Ogata Atsushi、Muraoka Kenji、Habe Hiroshi、Hori Tomoyuki
    • Journal Title

      The ISME Journal

      Volume: 12 Issue: 10 Pages: 2376-2388

    • DOI

      10.1038/s41396-018-0201-2

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Burkholderia insecticola sp. nov., a gut symbiotic bacterium of the bean bug Riptortus pedestris2018

    • Author(s)
      Takeshita Kazutaka、Tamaki Hideyuki、Ohbayashi Tsubasa、Meng Xian-Ying、Sone Teruo、Mitani Yasuo、Peeters Charlotte、Kikuchi Yoshitomo、Vandamme Peter
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology

      Volume: 68 Issue: 7 Pages: 2370-2374

    • DOI

      10.1099/ijsem.0.002848

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The roles of antimicrobial peptide, rip-thanatin, in the midgut of Riptortus pedestris2018

    • Author(s)
      Park Kyoung-Eun、Jang Seong Han、Lee Junbeom、Lee Seung Ah、Kikuchi Yoshitomo、Seo Young-su、Lee Bok Luel
    • Journal Title

      Developmental & Comparative Immunology

      Volume: 78 Pages: 83-90

    • DOI

      10.1016/j.dci.2017.09.009

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Metabolic Integration of Bacterial Endosymbionts through Antimicrobial Peptides2017

    • Author(s)
      Mergaert Peter、Kikuchi Yoshitomo、Shigenobu Shuji、Nowack Eva C.M.
    • Journal Title

      Trends in Microbiology

      Volume: 25 Issue: 9 Pages: 703-712

    • DOI

      10.1016/j.tim.2017.04.007

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Heterologous Expression, Biosynthetic Studies, and Ecological Function of the Selective Gq-Signaling Inhibitor FR9003592017

    • Author(s)
      Crusemann Max、Reher Raphael、Schamari Isabella et al.
    • Journal Title

      Angewandte Chemie International Edition

      Volume: 57 Issue: 3 Pages: 836-840

    • DOI

      10.1002/anie.201707996

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Unforeseen swimming and gliding mode of an insect gut symbiont, Burkholderia sp. RPE64, with wrapping of the flagella around its cell body2017

    • Author(s)
      Kinosita Yoshiaki、Kikuchi Yoshitomo、Mikami Nagisa、Nakane Daisuke、Nishizaka Takayuki
    • Journal Title

      The ISME Journal

      Volume: 12 Issue: 3 Pages: 838-848

    • DOI

      10.1038/s41396-017-0010-z

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Riptortus pedestris and Burkholderia symbiont: an ideal model system for insect-microbe symbiotic associations2016

    • Author(s)
      竹下和貴、菊池義智
    • Journal Title

      Research in Microbiology

      Volume: 168 Issue: 3 Pages: 175-187

    • DOI

      10.1016/j.resmic.2016.11.005

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ultra-high-sensitivity stable-isotope probing of rRNA by high-throughput sequencing of isopycnic centrifugation gradients2015

    • Author(s)
      青柳 智、花田 智、伊藤 英臣、佐藤 由也、尾形 敦、Michael W. Friedrich、菊池 義智、堀 知行
    • Journal Title

      ENVIRONMENTAL MICROBIOLOGY REPORTS

      Volume: 7 Issue: 2 Pages: 282-287

    • DOI

      10.1111/1758-2229.12243

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] High-Resolution Dynamics of Microbial Communities during Dissimilatory Selenate Reduction in Anoxic Soil2015

    • Author(s)
      Ronald R Navarro, Tomo Aoyagi, Makoto Kimura, Hideomi Itoh, Yuya Sato, Yoshitomo Kikuchi, Atsushi Ogata, Tomoyuki Hori
    • Journal Title

      Environmental science & technology

      Volume: 49 Issue: 13 Pages: 7684-7691

    • DOI

      10.1021/es505210p

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Insecticide applications to soil contribute to the development of Burkholderia mediating insecticide resistance in stinkbugs2015

    • Author(s)
      Kanako Tago, Yoshitomo Kikuchi, Sinji Nakaoka, Chie Katsuyama, Masahito Hayatsu
    • Journal Title

      Molecular ecology

      Volume: 24 Issue: 14 Pages: 3766-3778

    • DOI

      10.1111/mec.13265

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ultrabithorax is essential for bacteriocyte development2015

    • Author(s)
      Yu Matsuura, Yoshitomo Kikuchi, Toru Miura, Takema Fukatsu
    • Journal Title

      Proceedings of the National Academy of Sciences of U.S.A.

      Volume: 112 Issue: 30 Pages: 9376-9381

    • DOI

      10.1073/pnas.1503371112

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bacterial cell motility ofBurkholderia gut symbiont is required to colonize the insect gut2015

    • Author(s)
      Jun Beom Lee, Jin Hee Byeon, Ho Am Jang, Jiyeun Kate Kim, Jin Wook Yoo, Yoshitomo Kikuchi, Bok Luel Lee
    • Journal Title

      FEBS letters

      Volume: 589 Issue: 19PartB Pages: 2784-2790

    • DOI

      10.1016/j.febslet.2015.08.022

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Insect’s intestinal organ for symbiont sorting2015

    • Author(s)
      Tsubasa Ohbayashi, Kazutaka Takeshita, Wataru Kitagawa, Naruo Nikoh, Ryuichi Koga, Xian-Ying Meng, Kanako Tago, Tomoyuki Hori, Masahito Hayatsu, Kozo Asano, Yoichi Kamagata, Bok Luel Lee, Takema Fukatsu, Yoshitomo Kikuchi
    • Journal Title

      Proceedings of the National Academy of Sciences of U.S.A.

      Volume: 112 Issue: 37

    • DOI

      10.1073/pnas.1511454112

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evolutionary transition in symbiotic syndromes enabled diversification of phytophagous insects on an imbalanced diet2015

    • Author(s)
      Sailendharan Sudakaran, Franziska Retz, Yoshitomo Kikuchi, Christian Kost, Martin Kaltenpoth
    • Journal Title

      The ISME journal

      Volume: 9 Issue: 12 Pages: 2587-2604

    • DOI

      10.1038/ismej.2015.75

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Burkholderia of Plant-Beneficial Group are Symbiotically Associated with Bordered Plant Bugs (Heteroptera: Pyrrhocoroidea: Largidae)2015

    • Author(s)
      Kazutaka Takeshita, Yu Matsuura, Hideomi Itoh, Ronald Navarro, Tomoyuki Hori, Teruo Sone, Yoichi Kamagata, Peter Mergaert, Yoshitomo Kikuchi
    • Journal Title

      Microbes and Environments

      Volume: 30 Issue: 4 Pages: 321-329

    • DOI

      10.1264/jsme2.me15153

    • NAID

      130005116033

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] A novel function of Duox in insect-microbe gut symbiosis: development of trachea network and stabilization of symbiont colonization2019

    • Author(s)
      Jang Seonghan,菊池 義智
    • Organizer
      第63回日本応用動物昆虫学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 腸内細菌による殺虫剤のin vivo解毒プロセスの解明2019

    • Author(s)
      佐藤 由也,Jang Seonghan,伊藤 英臣,堀 知行,菊池 義智
    • Organizer
      第63回日本応用動物昆虫学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Molecular bases of the stinkbug-Burkholderia gut symbiosis2018

    • Author(s)
      菊池 義智
    • Organizer
      2018 ESA, ESC and ESBC Joint Annual Meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 共生微物による害虫の進化~そ多様性と機能2018

    • Author(s)
      菊池 義智
    • Organizer
      平成 30 年度(第 28 回)植物細菌病談話会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 昆虫のプロバイオティクス2018

    • Author(s)
      菊池 義智
    • Organizer
      2018年度日本乳酸菌学会セミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Hidden diversity under ground: ant- and nest-associated bacterial communities revealed by meta-genomic analyses2018

    • Author(s)
      下地 博之,伊藤 英臣,松浦 優,菊池 義智
    • Organizer
      International Union for the Study of Social Insects 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ”害虫”の誕生と共生微生物2018

    • Author(s)
      菊池 義智
    • Organizer
      2018年度北日本支部札幌シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Fish egg-associated microbiota acquired from ambient soils 水生動物の卵が土壌から獲得する細菌叢2018

    • Author(s)
      佐野 友紀,木原 稔,堀 知行,曾根 輝雄,菊池 義智,伊藤 英臣
    • Organizer
      日本微生物生態学会第32回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Diversity and distribution of microbiota associated with medaka fish メダカ常在細菌叢の多様性と分布 : エラ、体表、ヒレ、腸、卵に発達する細菌叢の解明2018

    • Author(s)
      伊藤 英臣,堀 知行,菊池 義智
    • Organizer
      日本微生物生態学会第32回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Intracellular symbiosis in lygaeoid species 腸内から細胞内へ:ナガカメムシの細胞内共生進化2018

    • Author(s)
      栗原 駿太,竹下和貴,松浦 優,徳田岳,曾根 輝雄,伊藤 英臣,菊池 義智
    • Organizer
      日本微生物生態学会第32回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] "Ant- and nest-associated bacterial communities revealed by meta-genomic analyses メタゲノムで解き明かすトゲオオハリアリの細菌叢 -社会に潜むその多様性と分布-"2018

    • Author(s)
      下地 博之,伊藤 英臣,松浦 優,菊池 義智
    • Organizer
      日本微生物生態学会第32回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 共生における免疫研究の新展開2017

    • Author(s)
      菊池義智
    • Organizer
      日本比較免疫学会第 29 回学術集会
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 共生による昆虫の進化2017

    • Author(s)
      菊池義智
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 微生物生態を利用した害虫制御の展望2016

    • Author(s)
      菊池義智
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] Evolutionary innovation through symbiotic association with microorganisms2016

    • Author(s)
      菊池義智
    • Organizer
      日本昆虫学会・日本応用動物昆虫学会合同大会
    • Place of Presentation
      大阪府立大学(大阪府堺市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Mutualistic endosymbiosis in insects: its diversity and molecular basis2016

    • Author(s)
      菊池義智
    • Organizer
      第89回日本細菌学会総会
    • Place of Presentation
      大阪国際交流センター(大阪府大阪市)
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 共生微生物による昆虫の農薬抵抗性進化2015

    • Author(s)
      菊池義智
    • Organizer
      菌根研究会2015年大会
    • Place of Presentation
      とかちプラザ(北海道帯広市)
    • Year and Date
      2015-10-31
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] ホソヘリカメムシ-Burkholderia共生系からひも解く共生微生物の進化2015

    • Author(s)
      菊池義智
    • Organizer
      日本微生物生態学会第30回大会
    • Place of Presentation
      亀城プラザ(茨城県土浦市)
    • Year and Date
      2015-10-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] Role of symbiosis-specific antimicrobial peptides in the stinkbug-Burkholderia symbiosis2015

    • Author(s)
      菊池義智
    • Organizer
      2015 International Meeting of "Matryoshka-type Evolution"
    • Place of Presentation
      筑波大学(茨城県つくば市)
    • Year and Date
      2015-09-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Evolutionary innovation through endosymbiosis2015

    • Author(s)
      菊池義智
    • Organizer
      2015年日本数理生物学会/日中韓数理生物学コロキウム合同大会
    • Place of Presentation
      同志社大学(京都府京都市)
    • Year and Date
      2015-08-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 微生物が引き起こす昆虫消化管のダイナミックな形態変化2015

    • Author(s)
      菊池義智
    • Organizer
      日本進化学会第17回大会
    • Place of Presentation
      中央大学(東京都文京区)
    • Year and Date
      2015-08-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] Symbiont mechanisms for stabilizing an insect-microbe symbiosis with horizontal transmission2015

    • Author(s)
      菊池義智
    • Organizer
      8th Congress of the International Symbiosis Society
    • Place of Presentation
      University of Lisbon(リスボン、ポルトガル)
    • Year and Date
      2015-07-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] カメムシに殺虫剤抵抗性を賦与するBurkholderia共生細菌2015

    • Author(s)
      菊池義智
    • Organizer
      日本生物地理学会第70回年次大会
    • Place of Presentation
      立教大学(東京都豊島区)
    • Year and Date
      2015-04-12
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Remarks] 共生細菌が示す第3形態のべん毛運動を発見

    • URL

      http://www.aist.go.jp/aist_j/press_release/pr2017/pr20171225/pr20171225.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 菊池研究室

    • URL

      https://staff.aist.go.jp/y-kikuchi/index.html

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2020-03-30  

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