| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2018: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Outline of Final Research Achievements |
Here, we characterized Nocardia sp. strain E1 and Rhizobacter gummiphilus NS21, which are able to grow on natural rubber. The rubber degrading genes, lcp was identified in E1. When the lcp gene that is expressed by a constitutive promoter was introduced into strain E1, the enhancement of the rubber degradation was observed. It is suggested that the constitutive expression of lcp is effective for the rubber degradation ability. When purified Lcp was added into the reaction mixture including rubber and the cells of E1, the rubber degradation activity was increased. It is suggested that Lcp is directly involved in the rubber degradation in vitro.
The transcriptome analysis of strain NS21 revealed that the genes including the transformation of natural rubber to polyhydroxyalkanoate, are found in the genome. We consider that the results are important for development of recycling system for waste natural-rubber product.
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