New progress of influenza NA study by using new technique of sialidase fluorescence imaging
| Project/Area Number |
15H05644
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental and hygienic pharmacy
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| Research Institution | University of Shizuoka |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥24,570,000 (Direct Cost: ¥18,900,000、Indirect Cost: ¥5,670,000)
Fiscal Year 2018: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
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| Keywords | シアリダーゼ / ノイラミニダーゼ / インフルエンザウイルス / パラミクソウイルス / 抗インフルエンザ薬 / 薬剤耐性 / イメージング / 蛍光 / NA阻害薬 / 高感度 / 酵素 / ウイルス / 糖鎖 / 衛生 / 感染症 / 単離 / ウィルス / 蛍光イメージング / 感染 / バキュロウイルス / BTP3-Neu5Ac |
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| Outline of Final Research Achievements |
BTP3-Neu5Ac is a fluorescent imaging reagent for visualizing an enzymatic activity of neuraminidase and was able to histochemically visualize the activity expressed on influenza A and B viruses, some paramyxoviruses, and their infected cells. We developed an imaging method for selectively visualizing viruses and their infected cells possessing a resistance against anti-influenza drug (neuraminidase inhibitor). We also developed an easy and speedy method for sensitively detecting the drug resistance. We established a foundation for structure modifications that could improve properties of BTP3-Neu5Ac.
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| Academic Significance and Societal Importance of the Research Achievements |
A型・B型インフルエンザウイルスや一部のパラミクソウイルスが持つノイラミニダーゼの酵素活性の存在位置を蛍光イメージングする新技術であり、ノイラミニダーゼ機能の新たな解析手法である。この技術の応用法として開発した、抗インフルエンザ薬（ノイラミニダーゼ阻害薬）の耐性検出法は、薬剤耐性インフルエンザウイルスの監視や疫学情報の取得に貢献する。このイメージング技術の向上は、ウイルス感染を酵素活性という新たな視点から解析する手法を提供してウイルス研究を開拓・効率化させる。
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Report
(5 results)
Research Products
(204 results)
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[Journal Article] Down-regulation of glutamate release from hippocampal neurons by sialidase2018
Author(s)
Akira Minami, Ami Ishii, Sumika Shimba, Takahiro Kano, Eri Fujioka, Saki Sai, Nagisa Oshio, Sayaka Ishibashi, Tadanobu Takahashi, Yuuki Kurebayashi, Hiroaki Kanazawa, Nobuhiro Yuki, Tadamune Otsubo, Kiyoshi Ikeda and Takashi Suzuki
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Journal Title
J. Biochem.
Volume: 163
Issue: 4
Pages: 273-280
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Rapid regulation of sialidase activity in response to neural activity and sialic acid removal during memory processing in rat hippocampus.2017
Author(s)
Akira Minami, Yuko Meguro, Sayaka Ishibashi, Ami Ishii, Mako Shiratori, Saki Sai, Yuuki Horii, Hirotaka Shimizu, Hokuto Fukumoto, Sumika Shimba, Risa Taguchi, Tadanobu Takahashi, Tadamune Otsubo, Kiyoshi Ikeda and Takashi Suzuki.
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Journal Title
J. Biol. Chem.
Volume: 292
Issue: 14
Pages: 5645-5654
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Substrate Specificity of Equine and Human Influenza A Virus Sialidase to Molecular Species of Sialic Acid2016
Author(s)
Tadanobu Takahashi, Saori Unuma, Sawako Kawagishi, Yuuki Kurebayashi, Maiko Takano, Hiroki Yoshino, Akira Minami, Takashi Yamanaka, Tadamune Otsubo, Kiyoshi Ikeda, Takashi Suzuki.
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Journal Title
Biological and Pharmaceutical Bulletin
Volume: 39
Issue: 10
Pages: 1728-1733
DOI
NAID
ISSN
0918-6158, 1347-5215
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Easy and Rapid Detection of Mumps Virus by Live Fluorescent Visualization of Virus-Infected Cells2015
Author(s)
Tadanobu Takahashi, Takashi Agarikuchi, Yuuki Kurebayashi, Nona Shibahara, Chihiro Suzuki, Akiko Kishikawa, Keijo Fukushima, Maiko Takano, Fumie Suzuki, Hirohisa Wada, Tadamune Otsubo, Kiyoshi Ikeda, Akira Minami, Takashi Suzuki
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Journal Title
PLoS One
Volume: 10
Issue: 12
Pages: e0144038-e0144038
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Amino acid substitutions contributing to α2,6-sialic acid linkage binding specificity of human parainfluenza virus type 3 hemagglutinin-neuraminidase.2015
Author(s)
Fukushima K, Takahashi T, Ueyama H, Takaguchi M, Ito S, Oishi K, Minami A, Ishitsubo E, Tokiwa H, Takimoto T, Suzuki T.
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Journal Title
FEBS Lett.
Volume: 589
Issue: 11
Pages: 1278-1282
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Recombinant IgA specific for influenza A virus hemagglutinin: production, functional analysis and formation of secretory IgA.2015
Author(s)
Kentaro Shoji, Tadanobu Takahashi, Kohta Kurohane, Koki Iwata, Takeshi Matsuoka, Shogo Tsuruta, Takatomo Sugino, Masaki Miyake, Takashi Suzuki, Yasuyuki Imai
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Journal Title
Viral Immunol.
Volume: 28,
Issue: 3
Pages: 170-178
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Consecutive oral administration of Bifidobacterium longum MM-2 improves the defense system against influenza virus infection by enhancing natural killer cell activity in a murine model.2015
Author(s)
Kawahara T, Takahashi T, Oishi K, Tanaka H, Masuda M, Takahashi S, Takano M, Kawakami T, Fukushima K, Kanazawa H, Suzuki T.
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Journal Title
Microbol. Immunol.
Volume: 59
Issue: 1
Pages: 1-12
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] N-glycolylneuraminic acid on human epithelial cells prevents entry of influenza A viruses that possess N-glycolylneuraminic acid binding ability2018
Author(s)
Tadanobu Takahashi, Maiko Takano, Yuuki Kurebayashi, Midori Masuda, Sawako Kawagishi, Masahiro Takaguchi, Takashi Yamanaka, Akira Minami, Tadamune Otsubo, Kiyoshi Ikeda, Takashi Suzuki
Organizer
Sialoglyco 2018
Related Report
Int'l Joint Research
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