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Screening and analysis of glial factors for neuroprotection

Research Project

Project/Area Number 15H05648
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionThe University of Tokyo

Principal Investigator

Kanemaru Kazunori  東京大学, 大学院医学系研究科(医学部), 助教 (10456105)

Co-Investigator(Renkei-kenkyūsha) IINO Masamitsu  日本大学, 医学部, 特任教授 (50133939)
OKUBO Yohei  東京大学, 大学院医学系研究科, 講師 (40422282)
SEKIYA Hiroshi  東京大学, 大学院医学系研究科, 助教 (40511374)
TANAKA Kenji  慶應義塾大学, 医学部, 准教授 (30329700)
TACHIKAWA Masanori  東北大学, 大学院薬学研究科, 准教授 (00401810)
Research Collaborator KITAJIMA Nami  東京大学, 大学院医学系研究科, 大学院生
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000)
Fiscal Year 2017: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Keywordsアストロサイト / カルシウムシグナル / カルシウムイメージング / 神経保護因子 / 脳損傷 / グリア細胞 / 反応性アストロサイト / 薬理学 / 神経細胞死
Outline of Final Research Achievements

In vivo calcium imaging analysis using transgenic mice expressing ultrasensitive calcium indicator in astrocytes revealed that injury-induced astrocytic calcium signals can spread in large area of the brain. This signaling pattern was disappeared in calcium signal deficient mice. Furthermore, we developed new calcium signal deficient mice by astrocyte specific expression of inositol triphosphate hydrolyzing enzyme, IP3 5-phosphatase. Using these transgenic mice, we screened glial calcium-dependent factors for neuroprotection and found that some molecules including glial cytoskeletal proteins may responsible for neuroprotection.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (23 results)

All 2018 2017 2016 2015

All Journal Article (11 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 8 results,  Open Access: 5 results,  Acknowledgement Compliant: 3 results) Presentation (12 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Journal Article] Role of endoplasmic reticulum-mediated Ca2+ signaling in neuronal cell death2018

    • Author(s)
      Okubo Yohei、Mikami Yoshinori、Kanemaru Kazunori、Iino Masamitsu
    • Journal Title

      Antioxid Redox Signal (in press)

      Volume: - Issue: 12 Pages: 1147-1157

    • DOI

      10.1089/ars.2018.7498

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Clarification of astrocytic function through Ca2+ imaging2016

    • Author(s)
      金丸和典
    • Journal Title

      生化学

      Volume: 88 Issue: 1 Pages: 128-134

    • DOI

      10.14952/SEIKAGAKU.2016.880128

    • ISSN
      0037-1017
    • Year and Date
      2016-02-25
    • Related Report
      2015 Annual Research Report
  • [Journal Article] Whisker experience-dependent mGluR signaling maintains synaptic strength in the mouse adolescent cortex.2016

    • Author(s)
      Kubota J., Mikami Y., Kanemaru K., Sekiya H., Okubo Y., and Iino M.
    • Journal Title

      Eur. J. Neurosci

      Volume: 44 Pages: 2004-2014

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nitric Oxide-induced Activation of the Type 1 Ryanodine Receptor Is Critical for Epileptic Seizure-induced Neuronal Cell Death2016

    • Author(s)
      Mikami, Y., Kanemaru, K., Okubo, Y., Nakaune, T., Suzuki, J., Shibata, K., Sugiyama, H., Koyama, R., Murayama, T., Ito, A., Yamazawa, T., Ikegaya, Y., Sakurai, T., Saito, N., Kakizawa, S. and Iino, M.
    • Journal Title

      EBioMedicine.

      Volume: 11 Pages: 253

    • DOI

      10.1016/j.ebiom.2016.08.020

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genetically encoded fluorescent indicators for organellar calcium imaging.2016

    • Author(s)
      Suzuki, J., Kanemaru, K. and Iino, M.
    • Journal Title

      Biophys J.

      Volume: 111 Issue: 6 Pages: 1119-1131

    • DOI

      10.1016/j.bpj.2016.04.054

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Genotype-phenotype correlations of malignant hyperthermia and central core disease mutations in the central region of the RYR1 channel.2016

    • Author(s)
      Murayama, T., Kurebayashi, N., Ogawa, H., Yamazawa, T., Oyamada, H., Suzuki, J., Kanemaru, K., Oguchi, K., Iino, M. and Sakurai, T.
    • Journal Title

      Hum Mutat.

      Volume: 37 Issue: 11 Pages: 1231-1241

    • DOI

      10.1002/humu.23072

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 高感度Ca2+指示タンパク質YC-Nano50発現マウスを用いた生体内アストロサイトの活動解析2016

    • Author(s)
      金丸和典
    • Journal Title

      日本薬理学雑誌

      Volume: 147 Pages: 190-193

    • NAID

      130005143836

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Visualization of Ca2+ filling mechanisms upon synaptic inputs in the endoplasmic reticulum of cerebellar Purkinje cells.2015

    • Author(s)
      Okubo, Y., Suzuki, J., Kanemaru, K., Nakamura, N., Shibata, T. and *Iino, M.
    • Journal Title

      J. Neurosci.

      Volume: 35 Issue: 48 Pages: 15837-15846

    • DOI

      10.1523/jneurosci.3487-15.2015

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Neuronal regulation of Schwann cell mitochondrial Ca2+ Signaling during myelination.2015

    • Author(s)
      Ino, D., Sagara, H., Suzuki, J., Kanemaru, K., Okubo, Y., and *Iino, M.
    • Journal Title

      Cell Rep.

      Volume: 12 Issue: 12 Pages: 1951-1959

    • DOI

      10.1016/j.celrep.2015.08.039

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Divergent Activity Profiles of Type 1 Ryanodine Receptor Channels Carrying Malignant Hyperthermia and Central Core Disease Mutations in the Amino-Terminal Region.2015

    • Author(s)
      Murayama T, Kurebayashi N, Yamazawa T, Oyamada H, Suzuki J, Kanemaru K, Oguchi K, Iino M, Sakurai T.
    • Journal Title

      PLoS One

      Volume: 10 Issue: 6 Pages: e0130606-e0130606

    • DOI

      10.1371/journal.pone.0130606

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 高感度Ca2+指示タンパク質を用いた生体内アストロサイト活動の可視化解析2015

    • Author(s)
      金丸和典、飯野正光
    • Journal Title

      生体の科学

      Volume: 66(6) Pages: 546-550

    • Related Report
      2015 Annual Research Report
  • [Presentation] In vivo analysis of astrocytic calcium signaling.2018

    • Author(s)
      Kazunori Kanemaru, Hiroshi Sekiya, Yohei Okubo, Masamitsu Iino
    • Organizer
      第5回グリアアセンブリ国際シンポジウム「グリアアセンブリによる脳機能の発現と病態」
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] カルシウムインジケーター発現マウスを用いた生体内β細胞カルシウムシグナル解析2017

    • Author(s)
      金丸 和典、太向 勇、北島 奈美、関谷 敬、飯野 正光
    • Organizer
      第90回 日本薬理学会年会
    • Place of Presentation
      長崎ブリックホール(長崎県長崎市)
    • Year and Date
      2017-03-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] カルシウムイメージングで切り拓くアストロサイト機能2017

    • Author(s)
      金丸 和典
    • Organizer
      第90回 日本薬理学会年会
    • Place of Presentation
      長崎ブリックホール(長崎県長崎市)
    • Year and Date
      2017-03-15
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Calcium imaging method using YC-Nano50 for the visualization of subtle and local activity of astrocytes in vivo2016

    • Author(s)
      金丸 和典
    • Organizer
      2nd "Young Glia" meeting in Homburg, Germany: Japanese-German YoungGlia collaborative meeting for mutual research exchange
    • Place of Presentation
      ザールランド大 (ドイツ)
    • Year and Date
      2016-05-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 超高感度カルシウムセンサーを発現するマウスを用いた生体内アストロサイトのイメージング解析2016

    • Author(s)
      金丸 和典、関谷 敬、北島 奈美、高木 美貴、田中 謙二、飯野 正光
    • Organizer
      第89回 日本薬理学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-03-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 一酸化窒素によるリアノジン受容体を介するCa2+放出が神経細胞のミトコンドリア機能に及ぼす影響2016

    • Author(s)
      中畝 拓哉, 鈴木 純二, 三上 義礼, 金丸 和典, 飯野 正光
    • Organizer
      第89回 日本薬理学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-03-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] オルガネラ内腔Ca2+インジケーターおよびCa2+インジケーター発現マウスを用いたβ細胞Ca2+シグナル解析2016

    • Author(s)
      金丸 和典、太向 勇、北島 奈美、関谷 敬、飯野 正光
    • Organizer
      第135回 日本薬理学会関東部会
    • Place of Presentation
      アクトシティ浜松(静岡県浜松市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 生体内アストロサイトの微細で局所的なカルシウム活動を捉えるイメージング技術2015

    • Author(s)
      金丸 和典、関谷 敬、北島 奈美、高木 美貴、田中 謙二、飯野 正光
    • Organizer
      第58日本神経化学会大会
    • Place of Presentation
      大宮ソニックシティ(埼玉県大宮市)
    • Year and Date
      2015-09-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] 1型リアノジン受容体のS-ニトロシル化を介した神経細胞死2015

    • Author(s)
      三上 義礼、金丸 和典、小田 康弘、柿澤 昌、柴田 和輝、杉山 弘樹、小山 隆太、伊藤 明博、山澤 徳志子、渡辺 雅彦、池谷 裕二、斉藤 延人、飯野 正光
    • Organizer
      第38回 日本神経科学大会
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Year and Date
      2015-07-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 生体内アストロサイトの微細なカルシウム活動を捉える新規イメージング技術2015

    • Author(s)
      金丸 和典
    • Organizer
      第38回 日本神経科学大会
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 脳血管平滑筋の生体内Ca2+イメージングによるアストロサイトの脳血流制御機構解析2015

    • Author(s)
      北島 奈美、関谷 敬、金丸 和典、田中 謙二、飯野 正光
    • Organizer
      第132回 日本薬理学会関東部会
    • Place of Presentation
      明海大学 浦安キャンパス(千葉県浦安市)
    • Year and Date
      2015-07-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] New Calcium Imaging Methods for the Visualization of Local Activities of Astrocytes in vivo2015

    • Author(s)
      Kazunori Kanemaru, Hiroshi Sekiya, Junji Suzuki, Kenji Tanaka, Masamitsu Iino
    • Organizer
      19th International Symposium on Calcium Binding Proteins and Calcium Function In Health and Disease
    • Place of Presentation
      アメリカ合衆国, Nashville
    • Year and Date
      2015-06-03
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2019-03-29  

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