| Budget Amount *help |
¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2018: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Outline of Final Research Achievements |
In this study, I investigated the mechanisms of phosphatidylserine (PS) exposure during apoptotsis by Xkr8 and its family memebrs. As a result, i found that Xkr8 is cleaved at its C-temrinal region of Caspase3, which results in dimerization of the molecules to be activated. the subunit analysis of Xkr8 identified BSG and NPTN as binding partners of Xkr8. Without binding to them, Xkr8 cannot reach to the plasma membrane, indicating that they function as chaperones to take Xkr8 to the plasma membrane. I also found that other Xkr family members are not dependent on BSG or NPTN to localize to the plasma membrane, suggesting that other chapernes are required for for other family memebrs. Finally, the screening system to discover the chaperones were established.
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