Comprehensive transcriptome analysis of group A streptococci using the murine invasive model reveals a key gene network causing severe invasive diseases

• Project/Area Number: 15H05654
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including mycology)
• Research Institution: Jichi Medical University
• Principal Investigator: Watanabe Shinya 自治医科大学, 医学部, 准教授 (60614956)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000); Fiscal Year 2018: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2017: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2016: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
• Keywords: レンサ球菌 / 劇症型レンサ球菌感染症 / マウス感染モデル / ゲノム解析 / 遺伝子発現 / 劇症型感染症 / 発現解析 / RNA-seq / A群レンサ球菌 / 遺伝子発現解析 / 急性胃蜂窩織炎

Outline of Final Research Achievements

Group A streptococci (GAS) can acquire a hypervirulent phenotype in transitioning from localized to systemic infections. This involves mutations in global regulator genes, such as csrS, followed by broad transcriptional alterations. However, the key genes controlled by the regulators have not yet been fully determined. In this study, the entire genomes of randomly-selected animal-passed GAS colonies were sequenced, as was a sdaD2 strain. The significantly reduced csrS mutation frequency in sdaD2 was accompanied by increases in mutations of other regulatory genes, such as rocA. The in vivo transcriptome profiles of six mutated hypervirulent-associated regulators was evaluated by RNA sequencing to identify a set of genes responsible for the hypervirulent phenotype. Transcriptome analysis identified a network co-regulated by the regulators involved in transitioning to systemic disease. This gene cluster may be important in inducing severe systemic GAS infections.

Academic Significance and Societal Importance of the Research Achievements

A群レンサ球菌はヒトに咽頭に常在する細菌である。A群レンサ球菌は、冬季の咽頭炎の原因菌であるにも関わらず、劇症型溶血性レンサ球菌感染症（STSS）のような致死率の高い感染症も同時に起こす。STSSの患者から分離された株は、転写調節因子に変異があり病原因子が高発現していることがわかっている。本研究では、マウス感染モデルを用いて、転写調節因子に変異がある高病原株が感染時に発現上昇する遺伝子の一群を同定した。

Research Products

• [Journal Article] Complete Genome Sequence of the Methicillin-Resistant Staphylococcus aureus Strain JMUB3031, Isolated from a Patient with Fatal Community-Acquired Pneumonia (2019)
  • Author(s): Bintao Cui, Shinya Watanabe, Yusuke Sato’o, Fumiya Nihashi, Yoshifumi Aiba, Kotaro Kiga, Teppei Sasahara, Xin-Ee Tan, Moriyuki Kawauchi, Tanit Boonsiri, Kanate Thitiananpakorn, Yusuke Taki, Feng-Yu Li, Shiro Imokawa, Longzhu Cui
  • Journal Title: Microbiology Resource Announcements Volume: 8 Issue: 4 Pages: 1-2
  • DOI: 10.1128/mra.01652-18
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of compensatory mutations associated with restoration of daptomycin-susceptibility in daptomycin non-susceptible methicillin-resistant Staphylococcus aureus (2019)
  • Author(s): Izumo Kanesaka, Shingo Fujisaki, Yoshifumi Aiba, Shinya Watanabe, Takashi Mikawa, Akiko Kanayama Katsuse, Hiroshi Takahashi, Longzhu Cui, Intetsu Kobayashi
  • Journal Title: Journal of Infection and Chemotherapy Volume: 25 Issue: 1 Pages: 1-5
  • DOI: 10.1016/j.jiac.2018.09.009
  • Peer Reviewed / Open Access
• [Journal Article] Complete genome sequencing of three human clinical isolates of Staphylococcus caprae reveals virulence factors similar to those of S. epidermidis and S. capitis (2018)
  • Author(s): Watanabe Shinya、Aiba Yoshifumi、Tan Xin-Ee、Li Feng-Yu、Boonsiri Tanit、Thitiananpakorn Kanate、Cui Bintao、Sato’o Yusuke、Kiga Kotaro、Sasahara Teppei、Cui Longzhu
  • Journal Title: BMC Genomics Volume: 19 Issue: 1 Pages: 810-810
  • DOI: 10.1186/s12864-018-5185-9
  • Peer Reviewed / Open Access
• [Journal Article] 溶連菌感染症の歴史 (2018)
  • Author(s): 渡邊真弥，崔龍洙
  • Journal Title: 小児科 Volume: 59 Pages: 1511-8
  • Peer Reviewed
• [Journal Article] Optimized universal protocol for electroporation of both coagulase-positive and -negative Staphylococci (2018)
  • Author(s): Sato'o Yusuke、Aiba Yoshifumi、Kiga Kotaro、Watanabe Shinya、Sasahara Teppei、Hayakawa Yasuhiko、Cui Longzhu
  • Journal Title: Journal of Microbiological Methods Volume: 146 Pages: 25-32
  • DOI: 10.1016/j.mimet.2018.01.006
  • Peer Reviewed / Open Access
• [Journal Article] Complete Genome Sequence and Comparative Genomics of a Streptococcus pyogenes emm3 Strain M3-b isolated from a Japanese Patient with Streptococcal Toxic Shock Syndrome. (2017)
  • Author(s): Kohei Ogura, Shinya Watanabe, Teruo Kirikae, Tohru Miyoshi-Akiyama
  • Journal Title: Journal of Genomics Volume: 5 Pages: 71-74
  • DOI: 10.7150/jgen.20915
  • Peer Reviewed / Open Access
• [Journal Article] 次世代シーケンス（NGS）による劇症型レンサ球菌の病原性解析 (2017)
  • Author(s): 竹本訓彦，小倉康平，渡邊真弥，秋山徹
  • Journal Title: 日本化学療法学会誌 Volume: 65 Pages: 745-750
• [Journal Article] Complete genome sequence of Streptococcus pyogenes strain JMUB1235 isolated from an acute phlegmonous gastritis patient. (2016)
  • Author(s): Watanabe, S., Sasahara, T., Arai, N., Sasaki, K., Aiba, Y., Sato’o, Y., and Cui, L.
  • Journal Title: Genome Announcements. Volume: ４ Issue: 5
  • DOI: 10.1128/genomea.01133-16
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A mutation in the decoding region of 16S rRNA attenuates the virulence of Mycobacterium tuberculosis. (2016)
  • Author(s): Watanabe, S., Matsumura, K., Iwai, H., Funatogawa, K., Haishima, Y., Fukui, C., Okumura, K., Kato, M., Hashimoto, M., Teramoto, K., Kirikae, F., Miyoshi-Akiyama, T., and Kirikae, T.
  • Journal Title: Infection and Immunity. Volume: 84 Issue: 8 Pages: 2264-2273
  • DOI: 10.1128/iai.00417-16
  • Peer Reviewed / Open Access
• [Journal Article] Severe invasive streptococcal infection by Streptococcus pyogenes and Streptococcus dysgalactiae subsp. equisimilis. (2016)
  • Author(s): Watanabe S, Takemoto N, Ogura K, Miyoshi-Akiyama T.
  • Journal Title: Microbiology and Immunology Volume: 60 Issue: 1 Pages: 1-9
  • DOI: 10.1111/1348-0421.12334
  • NAID: 40020725365
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 試験管内で選択したカナマイシン耐性結核菌のマウスに対する病原性解析 (2019)
  • Author(s): 渡邊真弥
  • Organizer: 第92回 細菌学会総会
  • Invited
• [Presentation] Bacterial EndoMS/NucS acts as a clamp-mediated mismatch endonuclease to prevent replication errors (2019)
  • Author(s): 〇竹本訓彦，沼田格，末次正幸，堀野芽生，渡邊真弥，秋山徹
  • Organizer: 第92回 細菌学会総会
  • Invited
• [Presentation] 殺菌キメラファージの開発（２）－レプトトリキア科細菌由来のCRISPR-Cas13システムの機能解析 (2019)
  • Author(s): Bintao Cui，渡邊真弥，氣駕恒太朗，相羽由詞，河内護之，Tanit Boonsiri，Kanate Thitiananpakorn，佐藤祐介，Xin Ee Tan，〇崔龍洙
  • Organizer: 第92回 細菌学会総会
• [Presentation] Mechanisms of cross-resistant to daptomycin and vancomycin in MRSA (2019)
  • Author(s): 〇Kanate Thitiananpakorn，相羽由詞，Xin Ee Tan，渡邊真弥，氣駕恒太朗，佐藤祐介，河内護之，Tanit Boonsiri，Bintao Cui，崔龍洙
  • Organizer: 第92回 細菌学会総会
• [Presentation] 黄色ブドウ球菌のSaPI-phageシステムを利用した殺菌キメラファージの合成 (2019)
  • Author(s): 〇Xin Ee Tan，氣駕恒太朗，渡邊真弥，佐藤祐介，相羽由詞，河内護之，Kanate Thitiananpakorn，Víctor Rodrigo Ibarra Chávez，José R Penadés，崔龍洙
  • Organizer: 第92回 細菌学会総会
• [Presentation] Genetic Analysis of Highly b-lactam-resistant Mutants Generated from OS-MRSA (2019)
  • Author(s): 〇Tanit Boonsiri，渡邊真弥，Kanate Thitiananpakorn，佐藤祐介，相羽由詞，氣駕恒太朗，笹原鉄平， 李峰宇，Xin Ee Tan，崔龍洙
  • Organizer: 第92回 細菌学会総会
• [Presentation] 殺菌キメラファージの開発（６）－大腸菌に広く感染するファージの分離・同定 (2019)
  • Author(s): 〇李峰宇，氣駕恒太朗，渡邊真弥，佐藤祐介，相羽由詞，河内護之，Xin Ee Tan， Tanit Boonsiri，Kanate Thitiananpakorn，崔龍洙
  • Organizer: 第92回 細菌学会総会
• [Presentation] 殺菌キメラファージの開発（７）－ファージによる細菌感染症治療モデルの確立 (2019)
  • Author(s): 〇佐藤祐介，李峰宇，氣駕恒太朗，渡邊真弥，相羽由詞，河内護之，Xin Ee Tan， Tanit Boonsiri，Kanate Thitiananpakorn，崔龍洙
  • Organizer: 第92回 細菌学会総会
• [Presentation] 殺菌キメラファージの開発（８）－抗菌ファージの開発に資する耐性菌の収集とMRSAファージの分離と同定 (2019)
  • Author(s): 〇相羽由詞，Xin Ee Tan，Kanate Thitiananpakorn，渡邊真弥，氣駕恒太朗，佐藤祐介，Tanit Boonsiri，李峰宇，河内護之，崔龍洙
  • Organizer: 第92回 細菌学会総会
• [Presentation] 殺菌キメラファージの開発（４）－酵母を利用したキメラファージ合成技術の開発 (2019)
  • Author(s): 〇河内護之，氣駕恒太朗，李峰宇，Tanit Boonsiri，Xin Ee Tan，佐藤祐介，相羽由詞，Kanate Thitiananpakorn，渡邊真弥，崔龍洙
  • Organizer: 第92回 細菌学会総会
• [Presentation] 殺菌キメラファージの開発（１）－狙った細菌を選択的に殺菌する殺菌技術の開発 (2019)
  • Author(s): 〇氣駕恒太朗，李峰宇，Xin Ee Tan，佐藤祐介，渡邊真弥，相羽由詞，Víctor Rodrigo Ibarra Chávez，José R Penadés，鈴木仁人，崔龍洙
  • Organizer: 第92回 細菌学会総会
• [Presentation] 毒素性ショック症候群を発症した黄色ブドウ球菌の毒素産生制御機構の解明 (2019)
  • Author(s): 〇瀧雄介，渡邊真弥，佐藤祐介，李峰宇，Kanate Thitiananpakorn，Tanit Boonsiri，相羽由詞，氣駕恒太朗，崔龍洙
  • Organizer: 第92回 細菌学会総会
• [Presentation] 緑膿菌に広く感染するファージの分離・同定 (2019)
  • Author(s): 〇渡邊真弥，李峰宇，氣駕恒太朗，相羽由詞，佐藤祐介，Xin Ee Tan，河内護之，Tanit Boonsiri，Kanate Thitiananpakorn，崔龍洙
  • Organizer: 第92回 細菌学会総会
• [Presentation] 高病原化A群レンサ球菌特異的検出法の開発 (2019)
  • Author(s): 〇堀野芽生，竹本訓彦，渡邊真弥，秋山徹
  • Organizer: 第92回 細菌学会総会
• [Presentation] Oxacillin感性mecA陽性黄色ブドウ球菌のβラクタム薬感性機構の解析 (2019)
  • Author(s): 〇渡邊真弥，Tanit Boonsiri，Kanate Thitiananpakorn，相羽由詞，佐藤祐介，李峰宇，氣駕恒太朗，笹原鉄平，崔龍洙
  • Organizer: 第30回日本臨床微生物学会総会
• [Presentation] walk mutation is responsible for the reverse cross-resistance of Staphylococcus capitis against vancomycin and teicoplanin (2018)
  • Author(s): Sato’o, Y., Shoji, M., Watanabe, S., Aiba, Y., Kiga, K., Kikuchi, K., Hiramatsu, K., and ○Cui, L.
  • Organizer: the 18th International Symposium on Staphylococci and Staphylococcal Infections (ISSSI2018)
  • Int'l Joint Research
• [Presentation] GENETIC EVALUATION OF HIGHLY b-LACTAM-RESISTANT MUTANTS OF OXACILLIN-SUSCEPTIBLE MECA POSITIVE STAPHYLOCOCCUS AUREUS (2018)
  • Author(s): Boonsiri, T., Watanabe, S., Thitiananpakorn, K., Sato’o, Y., Aiba, Y., Kiga, K., Sasahara, T. Li, FY., and Cui, L.
  • Organizer: the 18th International Symposium on Staphylococci and Staphylococcal Infections (ISSSI2018)
  • Int'l Joint Research
• [Presentation] On genetic diversity of b-lactam-resistant population of MRSA. (2018)
  • Author(s): Aiba, Y., Watanabe, S., Sato’o, Y., Kiga, K., Sasahara, T. Thitiananpakorn, K., Boonsiri, T. and Cui, L.
  • Organizer: the 18th International Symposium on Staphylococci and Staphylococcal Infections (ISSSI2018)
  • Int'l Joint Research
• [Presentation] GENETIC MECHANISMS LINKED TO DAPTOMYCIN AND VANCOMYCIN CROSS-RESISTANCE OF MRSA. (2018)
  • Author(s): Thitiananpakorn, K., Aiba, Y., Watanabe, S., Sato’o, Y., Kiga, K., Sasahara, T. Boonsiri, T. and Cui, L.
  • Organizer: the 18th International Symposium on Staphylococci and Staphylococcal Infections (ISSSI2018)
  • Int'l Joint Research
• [Presentation] COMPLETE GENOME SEQUENCES OF STAPHYLOCOCCUS CAPRAE STRAINS ISOLATED FROM HUMAN AND COMPARATIVE GENOMICS OF S. EPIDERMIDIS, S. CAPITIS AND S. CAPRAE. (2018)
  • Author(s): Watanabe, S., Aiba, Y., Tan, XE., Li, FY., Boonsiri, T., Thitiananpakorn, K., Cui, B., Sato’o, Y., Kiga, K., Sasahara, T. and Cui, L.
  • Organizer: the 18th International Symposium on Staphylococci and Staphylococcal Infections (ISSSI2018)
  • Int'l Joint Research
• [Presentation] Genetic determinants responsible for acquisition of b-lactam resistance in oxacillin-susceptible mecA-positive Staphylococcus aureus. (2018)
  • Author(s): Boonsiri Tanit, Shinya Watanabe, Thitiananpakorn Kanate, Yusuke Sato’o, Yoshifumi Aiba, Kotaro Kiga, Teppei Sasahara, XinEe Tan, Bintao Cui, Feng-Yu Li and Longzhu Cui
  • Organizer: The 4th JMU Workshop for Graduate Students
• [Presentation] On the genetic alterations conferring b-lactam resistance in oxacillin-susceptible mecA-positive Staphylococcus aureus． (2018)
  • Author(s): Boonsiri Tanit, Shinya Watanabe, Thitiananpakorn Kanate, Yusuke Sato’o, Yoshifumi Aiba, Kotaro Kiga, Teppei Sasahara, XinEe Tan, Bintao Cui, Feng-Yu Li and Longzhu Cui
  • Organizer: 第63回日本ブドウ球菌研究会
• [Presentation] Staphylococcus capitisにおけるバンコマイシン／テイコプラニン耐性の乖離機構の解明 (2018)
  • Author(s): 佐藤祐介，鐘司光貴，渡邊真弥，相羽由詞，氣駕恒太朗，菊池賢，平松啓一，崔龍洙
  • Organizer: 第63回日本ブドウ球菌研究会
• [Presentation] MRSAのβラクタム系抗菌薬耐性における遺伝的多様性 (2018)
  • Author(s): 相羽由詞，Kanate Thitiananpakorn，渡邊真弥，Tanit Boonsiri，佐藤祐介，氣駕恒太朗，李峰宇，笹原鉄平，崔龍洙
  • Organizer: 第63回日本ブドウ球菌研究会
• [Presentation] 次世代技術を用いた細菌学研究と細菌検査への応用～質量分析から次世代シーケンサーまで～ (2018)
  • Author(s): 渡邊真弥
  • Organizer: 第45回臨床検査技師研修会
• [Presentation] Oxacillin感性mecA陽性黄色ブドウ球菌のOxacillin感性機構の解析 (2018)
  • Author(s): 渡邊真弥，Tanit Boonsiri，Kanate Thitiananpakorn，相羽由詞，佐藤祐介，李峰宇，氣駕恒太朗，笹原鉄平，崔龍洙
  • Organizer: 第17回自治医科大学シンポジウム
• [Presentation] Establishment of a highly efficient gene transformation protocol for clinical Staphylococcus isolates (2018)
  • Author(s): Yusuke Sato’o, Yoshifumi Aiba, Kotaro Kiga, Shinya Watanabe, Teppei Sasahara, Yasuhiko Hayakawa, and Longzhu Cui
  • Organizer: 第17回自治医科大学シンポジウム
• [Presentation] Elucidating the genetic mechanism associated with daptomycin and vancomycin cross-resistance in MRSA (2018)
  • Author(s): Kanate Thitiananpakorn, Yoshifumi Aiba, Shinya Watanabe, Yusuke Sato’o, Kotaro Kiga, Teppei Sasahara, Tanit Boonsiri, Xin-Ee Tan, Bintao Cui, Feng-yu Li, and Longzhu Cui
  • Organizer: 第17回自治医科大学シンポジウム
• [Presentation] Genetic determinants of high level b-lactam resistance in oxacillin-susceptible mecA-positive Staphylococcus aureus (2018)
  • Author(s): Boonsiri Tanit, Watanabe Shinya, Thitiananpakorn Kanate, Sato’o Yusuke, Aiba Yoshifumi, Kiga Kotaro, Sasahara Teppei, XinEe Tan, Bintao Cui, Feng-Yu Li and Cui Longzhu
  • Organizer: 第17回自治医科大学シンポジウム
• [Presentation] Development of bacteriophage carrying CRISPR-Cas system as a novel anti-bacterial agent (2018)
  • Author(s): 31.Feng-yu Li, Kotaro Kiga, Xin-Ee Tan, Moriyuki Kawauchi, Shinya Watanabe, Yusuke Sato’o, Yoshifumi Aiba, Tanit Boonsiri, Kanate Thitiananpakorn, Bintao Cui, Teppei Sasahara and Longzhu Cui
  • Organizer: 第17回自治医科大学シンポジウム
• [Presentation] MRSA N315株ヘテロ細胞集団の遺伝学的解析 (2018)
  • Author(s): 相羽由詞，渡邊真弥，氣駕恒太朗，笹原鉄平，崔龍洙
  • Organizer: 第66回日本化学療法学会学術講演会 第66回日本化学療法学会総会 合同学会
• [Presentation] Oxacillin感性を示すmecA陽性黄色ブドウ球菌のmecA制御機構の解析 (2018)
  • Author(s): 渡邊真弥，相羽由詞，氣駕恒太朗，笹原鉄平，崔龍洙
  • Organizer: 第66回日本化学療法学会学術講演会 第66回日本化学療法学会総会 合同学会
• [Presentation] Genetic mechanisms of highβ-lactam resistance in Methicillin-resistant Staphylococcus aureus (2018)
  • Author(s): ○Boonsiri Tanit，渡邊真弥，Thitiananpakorn Kanate，佐藤祐介，相羽由詞，氣駕恒太朗，李峰宇，笹原鉄平，崔龍洙
  • Organizer: 第91回日本細菌学会総会
• [Presentation] MRSAのβ-ラクタム系抗菌薬耐性における遺伝学的多様性の解析 (2018)
  • Author(s): ○相羽由詞，渡邊真弥，佐藤祐介，氣駕恒太朗，Kanate Thitiananpakorn， Tanit Boonsiri，李峰宇，笹原鉄平，崔龍洙
  • Organizer: 第91回日本細菌学会総会
• [Presentation] Identification of mutations associated with cross-resistance to daptomycin and vancomycin in MRSA (2018)
  • Author(s): ○Kanate Thitiananpakorn，相羽由詞，渡邊真弥，佐藤祐介，氣駕恒太朗，笹原鉄平，Tanit Boonsiri，李峰宇，崔龍洙
  • Organizer: 第91回日本細菌学会総会
• [Presentation] Oxacillin感性を示すmecA陽性黄色ブドウ球菌のβ-ラクタム薬感性化機構の解明 (2018)
  • Author(s): ○渡邊真弥，Boonsiri Tanit，Thitiananpakorn Kanate，相羽由詞，佐藤祐介，氣駕恒太朗，笹原鉄平，崔龍洙
  • Organizer: 第91回日本細菌学会総会
• [Presentation] ブドウ球菌属細菌における高効率エレクトロポレーション法の確立 (2018)
  • Author(s): ○佐藤祐介，相羽由詞，氣駕恒太朗，渡邊真弥，笹原鉄平，早川靖彦，崔龍洙
  • Organizer: 第91回日本細菌学会総会
• [Presentation] カナマイシン耐性に寄与する16SリボゾームRNAの解読領域の変異はと結核菌の弱毒化 (2017)
  • Author(s): ○渡邊真弥，松村和典，祝弘樹，舩渡川圭次，加藤雅子，切替富美子，秋山徹，崔龍洙，切替照雄
  • Organizer: 第90回日本細菌学会総会
  • Place of Presentation: 仙台国際センター展示棟、宮城県仙台市
  • Year and Date: 2017-03-19
• [Presentation] A群レンサ球菌（Streptococus pyogenes）における新規病原性（制御）因子の探索 (2017)
  • Author(s): ○竹本訓彦，渡邊真弥，秋山徹
  • Organizer: 第90回日本細菌学会総会
  • Place of Presentation: 仙台国際センター展示棟、宮城県仙台市
  • Year and Date: 2017-03-19
• [Presentation] A mutation in the decoding region of 16S rRNA attenuates the virulence of Mycobacterium tuberculosis. (2017)
  • Author(s): Watanabe, S., Matsumura, K., ○Iwai, H., Funatogawa, K., Haishima, Y., Fukui, C., Okumura, K., Kato, M., Hashimoto, M., Teramoto, K., Kirikae, F., Miyoshi-Akiyama, T., and Kirikae, T.
  • Organizer: Keystone Symposia, New Developments in Our Basic Understanding of Tuberculosis (A5)
  • Place of Presentation: Fairmont Hotel Vancouver, USA
  • Year and Date: 2017-01-14
  • Int'l Joint Research
• [Presentation] A mutation in the decoding region of 16S rRNA attenuates the virulence of Mycobacterium tuberculosis. (2017)
  • Author(s): Watanabe, S.#, Matsumura, K.#, ○Iwai, H.#, Funatogawa, K., Haishima, Y., Fukui, C., Okumura, K., Kato, M., Hashimoto, M., Teramoto, K., Kirikae, F., Miyoshi-Akiyama, T., and Kirikae, T.
  • Organizer: Keystone Symposia, New Developments in Our Basic Understanding of Tuberculosis (A5)
  • Int'l Joint Research
• [Presentation] Oxacillin感性を示すmecA陽性黄色ブドウ球菌のin vitro耐性化株の変異解析 (2017)
  • Author(s): ○渡邊真弥，Tanit Boonsiri，Kanate Thitiananpakorn，相羽由詞，佐藤祐介，氣駕恒太朗，李峰宇，笹原鉄平，崔龍洙
  • Organizer: 第64回日本化学療法学会東日本支部総会 第66回日本感染症学会東日本地方会学術集会
• [Presentation] 黄色ブドウ球菌におけるダプトマイシン非感性化機序の遺伝学的特徴 (2017)
  • Author(s): ○Kanate Thitiananpakorn，相羽由詞，渡邊真弥，Tanit Boonsiri，佐藤祐介，氣駕恒太朗，李峰宇，笹原鉄平，崔龍洙
  • Organizer: 第64回日本化学療法学会東日本支部総会 第66回日本感染症学会東日本地方会学術集会
• [Presentation] Oxacillin感性を示すmecA陽性黄色ブドウ球菌のOxacillin耐性化機構の解明 (2017)
  • Author(s): ○渡邊真弥，Tanit Boonsiri，Kanate Thitiananpakorn，相羽由詞，佐藤祐介，氣駕恒太朗，李峰宇，笹原鉄平，崔龍洙
  • Organizer: 第62回日本ブドウ球菌研究会
• [Presentation] Identification of gene mutations associated with reduced susceptibility to both daptomycin and vancomycin in Staphylococcus aureus (2017)
  • Author(s): ○Kanate Thitiananpakorn，Yoshifumi Aiba，Shinya Watanabe，Yusuke Sato’o，Boonsiri Tanit，Kotaro Kiga，Longzhu Cui
  • Organizer: 第62回日本ブドウ球菌研究会
• [Presentation] 結核菌のカナマイシン耐性に寄与する16SリボゾームRNAの解読領域の変異と病原性の変化 (2017)
  • Author(s): ○渡邊真弥，松村和典，祝弘樹，舩渡川圭次，加藤雅子，切替富美子，秋山徹，崔龍洙，切替照雄
  • Organizer: 第11回細菌学若手コロッセウム in つくば
• [Presentation] グリコペプチド耐性Staphylococcus capitisのゲノム解析 (2016)
  • Author(s): ○佐藤祐介，鐘司光貴，渡邊真弥，崔龍洙
  • Organizer: 日本細菌学会関東支部 インターラボセミナー2016 in 栃木
  • Place of Presentation: 自治医科大学 地域医療情報研修センター、栃木県下野市
  • Year and Date: 2016-11-21
• [Presentation] A 群レンサ球菌の侵襲性感染症発症時におけるゲノムの変化と遺伝子発現解析 (2016)
  • Author(s): ○渡邊真弥，小倉康平，切替照雄，崔龍洙，秋山徹
  • Organizer: 第99回 細菌学会関東支部会
  • Place of Presentation: 北里大学薬学部コンベンションホール、東京
  • Year and Date: 2016-10-06
  • Invited
• [Presentation] Genomic analysis of Staphylococcus capitis resistant to glycopeptide antibiotics. (2016)
  • Author(s): ○Yusuke Sato’o, Mitsutaka Shoji, Shinya Watanabe, Ken Kikuchi, Keiichi Hiramatsu, Longzhu Cui.
  • Organizer: 17th International Symposium on Staphylococci and Staphylococcal Infections (ISSSI).
  • Place of Presentation: Nine Tree Convention Center (Seoul, South Korea)
  • Year and Date: 2016-08-30
  • Int'l Joint Research
• [Presentation] Streptococcus pyogenesにおけるCovRS regulonの探索 (2016)
  • Author(s): ○竹本訓彦，渡邊真弥，秋山徹
  • Organizer: 第48回 レンサ球菌研究会
  • Place of Presentation: 長崎大学医学部 良順会館 専斎ホール、長崎県長崎市
  • Year and Date: 2016-07-08
• [Presentation] Complete Genome Sequence of a Streptococcus pyogenes emm3 Strain, M3-b, Isolated from a Japanese Patient with Streptococcal Toxic Shock Syndrome Case, and Comparative Genomics of S. pyogenes emm3 Strains. (2016)
  • Author(s): K. Ogura, Watanabe, S., Kirikae, T., and ○Miyoshi-Akiyama, T.
  • Organizer: asm microbe 2016.
  • Place of Presentation: the Boston Convention & Exhibition Center, USA
  • Year and Date: 2016-06-17
  • Int'l Joint Research
• [Presentation] A群レンサ球菌のマウス感染モデルを用いた発現解析 (2016)
  • Author(s): ○渡邊真弥，切替照雄，秋山徹
  • Organizer: 第89回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター（大阪府大阪市）
  • Year and Date: 2016-03-23
• [Presentation] RiboswitchによるRNA分解を介した遺伝子発現制御 (2016)
  • Author(s): ○竹本訓彦，渡邊真弥，田中裕也，乾将行，秋山徹
  • Organizer: 第89回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター（大阪府大阪市）
  • Year and Date: 2016-03-23
• [Presentation] A群レンサ球菌の高病原化スイッチング (2015)
  • Author(s): ○渡邊真弥，切替照雄，秋山徹
  • Organizer: BMB2015 第38回日本分子生物学会年会第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸国際展示場（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] A群レンサ球菌の劇症型感染症発症時における遺伝子発現ネットワーク解析 (2015)
  • Author(s): ○渡邊真弥，切替照雄，秋山徹
  • Organizer: 第9回細菌学若手コロッセウム
  • Place of Presentation: KKRホテル 敬天閣（鹿児島県鹿児島市）
  • Year and Date: 2015-11-23
• [Presentation] A群レンサ球菌の劇症型感染症発症時における遺伝子発現ネットワーク解析 (2015)
  • Author(s): ○渡邊真弥，切替照雄，秋山徹
  • Organizer: 日本細菌学会関東支部インターラボセミナー
  • Place of Presentation: 国立国際医療研究センター研究所（東京都新宿区）
  • Year and Date: 2015-08-06
• [Remarks] 自治医科大学 感染・免疫学講座 細菌学部門
  • URL: http://www.jichi.ac.jp/bacteriology/