| Budget Amount *help |
¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2018: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2017: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2016: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Outline of Final Research Achievements |
Group A streptococci (GAS) can acquire a hypervirulent phenotype in transitioning from localized to systemic infections. This involves mutations in global regulator genes, such as csrS, followed by broad transcriptional alterations. However, the key genes controlled by the regulators have not yet been fully determined. In this study, the entire genomes of randomly-selected animal-passed GAS colonies were sequenced, as was a sdaD2 strain. The significantly reduced csrS mutation frequency in sdaD2 was accompanied by increases in mutations of other regulatory genes, such as rocA. The in vivo transcriptome profiles of six mutated hypervirulent-associated regulators was evaluated by RNA sequencing to identify a set of genes responsible for the hypervirulent phenotype. Transcriptome analysis identified a network co-regulated by the regulators involved in transitioning to systemic disease. This gene cluster may be important in inducing severe systemic GAS infections.
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