• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of the Th17 cell-inducing bacteria from IBD patients

Research Project

Project/Area Number 15H05657
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionKeio University

Principal Investigator

Atarashi Koji  慶應義塾大学, 医学部(信濃町), 准教授 (60546787)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥24,180,000 (Direct Cost: ¥18,600,000、Indirect Cost: ¥5,580,000)
Fiscal Year 2017: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2016: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2015: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
KeywordsTh17細胞 / microbiota / IBD / 腸内細菌 / 粘膜免疫 / 炎症性腸疾患 / Th17
Outline of Final Research Achievements

Th17 cells play a role in host defense against extracellular pathogens and also pathogenesis of multiple inflammatory and autoimmune disorders. I have identified the human gut bacteria that could induce intestinal Th17 cells. Th17 cells were significantly increased in the colon of the germ-free mice inoculated with fecal samples from Ulcerative colitis (UC) patients. This Th17 cell induction was further enhanced when the mice were treated with Ampicillin (Amp) in the drinking water. To isolate Th17-inducing bacterial species, I cultured cecal contents from these UC feces colonized and Amp treated mice. I isolated 20 bacterial strains belonging to Clostridium, Bifidobacterium, Ruminococcus, and Bacteroides and inoculated germ-free mice with these 20 strains. The colonization of these 20 strains strongly induced a robust accumulation of Th17 cells in the colon. Furthermore, nine out of the 20 strains were significantly increased in the microbiomes of UC and Crohn’s disease subjects.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (9 results)

All 2018 2017 2016 2015

All Journal Article (5 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (4 results) (of which Invited: 4 results)

  • [Journal Article] Ectopic colonization of oral bacteria in the intestine drives TH1 cell induction and inflammation" Ectopic colonization of oral bacteria in the intestine drives TH1 cell induction and inflammationx2017

    • Author(s)
      Atarashi K, Suda W, Luo C, Kawaguchi T, Motoo I, Narushima S, Kiguchi Y, Yasuma K, Watanabe E, Tanoue T, Thaiss CA, Sato M, Toyooka K, Said HS, Yamagami H, Rice SA, Gevers D, Johnson RC, Segre JA, Chen K, Kolls JK, Elinav E, Morita H, Xavier RJ, Hattori M, Honda K.
    • Journal Title

      Science

      Volume: 358 Issue: 6361 Pages: 359-365

    • DOI

      10.1126/science.aan4526

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Maternal gut bacteria promote neurodevelopmental abnormalities in mouse offspring2017

    • Author(s)
      Kim S, Kim H, Yim YS, Ha S, Atarashi K, Tan TG, Longman RS, Honda K, Littman DR, Choi GB, Huh JR
    • Journal Title

      Nature

      Volume: 549 Issue: 7673 Pages: 528-532

    • DOI

      10.1038/nature23910

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A subpopulation of high IL-21-producing CD4(+) T cells in Peyer's Patches is induced by the microbiota and regulates germinal centers.2016

    • Author(s)
      Jones L, Ho WQ, Ying S, Ramakrishna L, Srinivasan KG, Yurieva M, Ng WP, Subramaniam S, Hamadee NH, Joseph S, Dolpady J, Atarashi K, Honda K, Zolezzi F, Poidinger M, Lafaille JJ, Curotto de Lafaille MA.
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 30784-30784

    • DOI

      10.1038/srep30784

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A Diet-dependent, microbiota-independent regulation of IL-10-producing lamina propria macrophages in the small intestine2016

    • Author(s)
      Ochi T, Feng Y, Kitamoto S, Nagao-Kitamoto H, Kuffa P, Atarashi K, Honda K, Teitelbaum DH, Kamada N
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 27634-27634

    • DOI

      10.1038/srep27634

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Th17 Cell induction by adhesion of microbes to intestinal epithelial cells2015

    • Author(s)
      Atarashi, K., Tanoue, T., Ando. M., Kamada, N., Nagano, Y., Narushima, S., Suda, W., Imaoka, A., Setoyama, H., Nagamori, T., Ishikawa, E., Shima, T., Hara, T., Kado, S., Jinnohara, T., Ohno, H., Kondo, T. et al.
    • Journal Title

      Cell

      Volume: 163 Issue: 2 Pages: 367-380

    • DOI

      10.1016/j.cell.2015.08.058

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 口腔由来クレブシエラ菌の腸内定着と炎症誘導2018

    • Author(s)
      新幸二
    • Organizer
      第 27 回東京免疫フォーラム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] ヒト腸内フローラと免疫機能2017

    • Author(s)
      新幸二
    • Organizer
      (公社)日本実験動物学会 維持会員懇談会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 口腔細菌による腸管免疫系活性化機構の解明2016

    • Author(s)
      新幸二
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2016-09-26
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 口腔内細菌による腸管免疫活性化機構の解明2016

    • Author(s)
      新幸二
    • Organizer
      フォーラム2016 衛生薬学・環境トキシコロジー
    • Place of Presentation
      昭和大学旗の台キャンパス(東京都品川区)
    • Year and Date
      2016-09-26
    • Related Report
      2016 Annual Research Report
    • Invited

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi