| Budget Amount *help |
¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2018: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2017: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Outline of Final Research Achievements |
Phospholipids are the main constituents of cell membranes and plasma lipoproteins. Based on structures, phospholipids are classified into classes such as phosphatidylcholine (PC) and phosphatidylinositol (PI). However, there has been no highly sensitive and high-throughput method for measuring phospholipids. In this study, in addition to our previously developed assays, we developed the enzymatic fluorometric assays for quantifying all major phospholipid classes. Furthermore, by using these enzymatic fluorometric assays for measurements of all major phospholipid classes, we evaluated in detail the effects of cell density of enzyme expression on phospholipid compositions in cultured cells. We also elucidated the mechanism of biliary phospholipid secretion.
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