| Budget Amount *help |
¥23,660,000 (Direct Cost: ¥18,200,000、Indirect Cost: ¥5,460,000)
Fiscal Year 2017: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2016: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2015: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Outline of Final Research Achievements |
FUS gene mutation in 13 families was identified by accumulation of familial ALS families. Among them, skin biopsy of FUS-related ALS (FUS-ALS) in two families was carried out with consent in accordance with the procedure approved by the Ethics Committee of the University. From this, primary culture fibroblasts were established and iPS cells were established at a collaborating researcher's facility. We analyzed RNA sequence sequence after motor neuron differentiation and reported to Stem Cell Reports in 2016. We also produced isogenic lines using genome editing technology. In order to reproduce the characteristic long axon protrusions of motor neurons in the culture environment using the iPS cells produced, we also examined the condition in a novel microfluidic device. By RNAseq analysis, gene profiles expressed in cell bodies and axons were identified.
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