Optimization of human iPS cell derived liver bud transplantation
| Project/Area Number |
15H05677
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Yokohama City University |
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Principal Investigator |
TAKEBE Takanori 横浜市立大学, 先端医科学研究センター, 教授 (20612625)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2017: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2016: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2015: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
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| Keywords | 再生医学 / 移植外科学 / 移植 / 肝臓原基 |
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| Outline of Final Research Achievements |
Organoid technology provides a revolutionary paradigm towards therapy, yet to be applied in humans mainly because of the reproducibility and scalability challenges. Here, we overcome these limitations by evolving scalable organ bud production platform entirely from human iPSC. First, we identified effective progenitor populations for generating liver buds in a highly reproducible manner: hepatic endoderm, endothelium and septum mesenchyme. Furthermore, we achieved human scalability by developing an omni-well-array culture platform for mass-producing homogenous and miniaturized liver buds on a large scale (>108-scale). Vascularized and functional liver tissues generated entirely from iPSC significantly improved subsequent hepatic functionalization, enabling functional rescue against acute liver failure via transplantation. Overall, our study provides a stringent manufacture platform for multi-cellular organoid supply, thus facilitating clinical and pharmaceutical applications.
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Report
(4 results)
Research Products
(100 results)
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[Journal Article] Massive and Reproducible Production of Liver Buds Entirely from Human Pluripotent Stem Cells2017
Author(s)
Takebe T*, Sekine K, Kimura M, Yoshizawa E, Funayama S, Nakanishi N, Hisai T, Kobayashi T, Mori A, Ayano S, Ejiri Y, Amimoto N, Yamazaki Y, Ogawa S, Ishikawa M, Kiyota Y, Ueno Y, Taniguchi H
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Journal Title
Cell Reports
Volume: 21
Pages: 2661-2670
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Multilineage communication regulates human liver bud development from pluripotency2017
Author(s)
Camp JG, Sekine K, Gerber T, Loeffler-Wirth H, Binder H, Gac M, Kanton S, Kageyama J, Damm G, Seehofer D, Belicova L, Bickle M, Barsacchi R, Okuda R, Yoshizawa E, Kimura M, Ayabe H, Taniguchi H, Takebe T*, Treutlein B
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Journal Title
Nature
Volume: 546
Pages: 533-534
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Multilineage communication regulates human liver bud self-organization from pluripotency.2017
Author(s)
Camp JG, Sekine K, Gerber T, Loeffler-Wirth H, Binder H, Gac M, Kanton S, Kageyama J, Damm G, Seehofer D, Belicova L, Bickle M, Barsacchi R, Okuda R, Yoshizawa E, Kimura M, Ayabe H, Taniguchi H, Takebe T, Treutlein B
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Journal Title
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Vascularized And Complex Organ Buds From Diverse Tissues Via Mesenchymal Cell-Driven Condensation.2015
Author(s)
Takanori Takebe, Masahiro Enomura, Emi Yoshizawa, Masaki Kimura, Hiroyuki Koike, Ueno Yasuharu, Takahisa Matsuzaki, Takashi Yamazaki, Takafumi Toyohara, Kenji Osafune, Hiromitsu Nakauchi, Hiroshi Y. Yoshikawa, and Hideki Taniguchi
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Journal Title
Cell Stem Cell.
Volume: 16
Pages: 556-565
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] ミニ臓器創出と臨床応用2017
Author(s)
武部貴則
Organizer
新化学技術推進協会（JACI）講演会
Place of Presentation
新化学技術推進協会（東京都千代田区）
Year and Date
2017-01-23
Related Report
Invited
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[Presentation] 臓器創出への展望2016
Author(s)
武部貴則
Organizer
大塚製薬講演
Place of Presentation
大塚ホールディングス株式会社（東京都港区）
Year and Date
2016-05-16
Related Report
Invited
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[Book] 肝胆膵2015
Author(s)
谷口英樹、武部貴則
Total Pages
8
Publisher
アークメディア
Related Report
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[Patent(Industrial Property Rights)] 細胞選別法2017
Inventor(s)
松﨑賢寿，谷口英樹，武部貴則
Industrial Property Rights Holder
松﨑賢寿，谷口英樹，武部貴則
Industrial Property Rights Type
特許
Industrial Property Number
2017-043744
Filing Date
2017-03-08
Application Year
2017
Related Report
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