| Budget Amount *help |
¥23,660,000 (Direct Cost: ¥18,200,000、Indirect Cost: ¥5,460,000)
Fiscal Year 2017: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2016: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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| Outline of Final Research Achievements |
As a result of examining cytokine secretion from ADMPC, cytokine having the curative properties of dissolving hepatic fibrosis was secreted in a greater amount than fibroblasts or adipose tissue-derived cells. This mechanism might be Mode-of-Action.
In order to investigate conditions for producing cell sheets, culturing ADMPCs with TGF-β were examined. The efficiency of producing cell sheets were good, but unfortunately the expression of anti-inflammatory cytokines decreased. It was found to be unfavor able for preparing cells for treating to the liver cirrhosis. So, we improved pretreat ment method for temperature sensitive culture dish and increased producing cell sheets efficiency.
When the ADMPC sheets were transplanted into a liver cirrhosis model rats, compared with the control group, the fibrosis rate was significantly improved in the sheet administration group.
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