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To develop the effective treatment for hepatic fibrosis using Adipose Tissue-Derived.

Research Project

Project/Area Number 15H05679
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionNational Institutes of Biomedical Innovation, Health and Nutrition

Principal Investigator

OKURA Hanayuki  国立研究開発法人医薬基盤・健康・栄養研究所, 医薬基盤研究所 難治性疾患研究開発・支援センター, 研究調整専門員 (20589684)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥23,660,000 (Direct Cost: ¥18,200,000、Indirect Cost: ¥5,460,000)
Fiscal Year 2017: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2016: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Keywords脂肪組織由来多系統前駆細胞 / 細胞シート / 肝線維症 / 再生医療 / 再生医学 / 肝硬変 / ADMPC / 肝線維化 / ウイルス性肝炎 / 非ウイルス性肝炎 / 脂肪組織由来鯛系統前駆細胞
Outline of Final Research Achievements

As a result of examining cytokine secretion from ADMPC, cytokine having the curative properties of dissolving hepatic fibrosis was secreted in a greater amount than fibroblasts or adipose tissue-derived cells. This mechanism might be Mode-of-Action.
In order to investigate conditions for producing cell sheets, culturing ADMPCs with TGF-β were examined. The efficiency of producing cell sheets were good, but unfortunately the expression of anti-inflammatory cytokines decreased. It was found to be unfavor able for preparing cells for treating to the liver cirrhosis. So, we improved pretreat ment method for temperature sensitive culture dish and increased producing cell sheets efficiency.
When the ADMPC sheets were transplanted into a liver cirrhosis model rats, compared with the control group, the fibrosis rate was significantly improved in the sheet administration group.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (32 results)

All 2018 2017 2016 2015

All Journal Article (15 results) (of which Peer Reviewed: 12 results,  Open Access: 7 results) Presentation (13 results) (of which Int'l Joint Research: 6 results) Book (2 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] The Rare Disease Bank of Japan: establishment, current status and future challenges.2018

    • Author(s)
      Tada M, M Hirata, Sakate R, Okura H, Matsuyama A.
    • Journal Title

      Human Cell

      Volume: - Issue: 3 Pages: 183-188

    • DOI

      10.1007/s13577-018-0204-3

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 再生医療等製品にかかる品質規格について―公表論文からの考察―2018

    • Author(s)
      大倉華雪 松山晃文
    • Journal Title

      医薬品医療機器レギュラトリーサイエンス

      Volume: -

    • Related Report
      2017 Annual Research Report
  • [Journal Article] 我が国の再生医療研究と国際状況2018

    • Author(s)
      大倉華雪 松山晃文
    • Journal Title

      CLINICALNEUROSCIENCE

      Volume: 36(3) Pages: 288-292

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Therapeutic potential of adipose tissue-derived multi-lineage progenitor cell-sheets on liver fibrosis2017

    • Author(s)
      Okura H, Morita M, Fujita M, Naba K, Takada N, Takagaki Y, Suzuki O, Matsuyama A.
    • Journal Title

      Journal of Translational Science

      Volume: 4(1) Issue: 6 Pages: 1-6

    • DOI

      10.15761/jts.1000200

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Trends of intractable disease research using biosamples.2017

    • Author(s)
      Tada M, Hirata M, Okura H, Yoshida S and Matsuyama A.
    • Journal Title

      Journal of Translational Science

      Volume: 4(1) Issue: 6 Pages: 1-5

    • DOI

      10.15761/jts.1000198

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Induced Pluripotent Stem Cells: Global Research Trends.2017

    • Author(s)
      Negoro T, Okura H, Matsuyama A.
    • Journal Title

      Biores Open Access

      Volume: 6(1) Issue: 1 Pages: 63-73

    • DOI

      10.1089/biores.2017.0013

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 再生医療等製品2017

    • Author(s)
      大倉華雪 松山晃文
    • Journal Title

      日本病院薬剤師会雑誌

      Volume: 5382 Pages: 146-151

    • NAID

      40021088436

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Currently available rapid microbial Tests for translational medicine.2016

    • Author(s)
      Okura H and Matsuyama A.
    • Journal Title

      Translational Biomedicine.

      Volume: 7(3) Issue: 3 Pages: 87-87

    • DOI

      10.21767/2172-0479.100087

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Adipose tissue-derived cellular medicinal products for ischemic heart disease.2016

    • Author(s)
      Okura H, Matsuyama A.
    • Journal Title

      Nihon Rinsho

      Volume: 74(Suppl4 Pt1) Pages: 671-675

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Spermine Treated-Adipose Tissue-Derived Multi-Lineage Progenitor Cells Improve Left Ventricular Dysfunction in a Swine Model of Chronic Myocardial Infarction.2016

    • Author(s)
      Okura H, Morita M, Fujita M, Naba K, Haseb-Takada N, Ichinose A, Matsuyama A.
    • Journal Title

      J Stem Cell Res Ther

      Volume: 6(2) Issue: 02 Pages: 326-326

    • DOI

      10.4172/2157-7633.1000326

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Regulatory aspect of pre-clinical studies for regenerative medicine.2016

    • Author(s)
      Okura H and Matsuyama A.
    • Journal Title

      Translational Medicine.

      Volume: 6(4) Issue: 04 Pages: 182-182

    • DOI

      10.4172/2161-1025.1000182

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Spermine Treated-Adipose Tissue-Derived Multi-Lineage Progenitor Cells Improve Left Ventricular Dysfunction in a Swine Model of Chronic Myocardial Infarction.2016

    • Author(s)
      Okura H., Morita M., Fujita M., Naba K., Hasebe-Takada N., Ichinose A., Matsuyama A.
    • Journal Title

      J Stem Cell Res Ther

      Volume: 6:2

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Trophic factors from adipose tissue-derived multi-lineage progenitor cells promote cytodifferentiation of periodontal ligament cells.2015

    • Author(s)
      Sawada K., Takedachi M., Yamamoto S., Morimoto C., Ozasa M., Iwayama T., Lee C.M., Okura H., Matsuyama A., Kitamura M., Murakami S.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: Aug 14;464(1) Issue: 1 Pages: 299-305

    • DOI

      10.1016/j.bbrc.2015.06.147

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of in vivo tumorigenicity tests using severe immunodeficient NOD/Shi-scid IL2Rγnull mice for detection of tumorigenic cellular impurities in human cell-processed therapeutic products.2015

    • Author(s)
      Kusakawa S., Machida K., Yasuda S., Takada N., Kuroda T., Sawada R., Okura H., Tsutsumi H., Kawamata S., Sato Y.
    • Journal Title

      Regenetive Therapy

      Volume: Dec;1(4) Pages: 30-37

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Allogeneic spermine treated-ADMPCs improve left ventricular dysfunction in a swine chronic MI model.2015

    • Author(s)
      Okura H., Takada N., Morita M., Fujita M., Naba K., Ichinose A., Matsuyama A.
    • Journal Title

      Proceedings of the 11th International Congress on Coronary Artery Disease. 2015.

      Volume: 1 Pages: 135-138

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] 臨床応用のためのMOAとPOC2018

    • Author(s)
      H Okura, M Morita, M Fujita, K naba, A Matsuyama.
    • Organizer
      World Congress on Toxicology and Pharmacology 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] iPS細胞の特許出願動向2017

    • Author(s)
      Y Morita,H Okura,A Matsuyama.
    • Organizer
      International Society for Stem Cell Research 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 脂肪組織由来多系統前駆細胞治療薬2017

    • Author(s)
      H Okura,M Morita,M Fujita,K naba,A Matsuyama.
    • Organizer
      Nature conferences Regeneration 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 「革新的医薬品・医療機器・再生医療製品実用化促進事業における再生医療製品開発ガイドラインの作成」「経冠動脈的投与再生医療等製品に関する評価指標」策定の経緯2017

    • Author(s)
      大倉 華雪
    • Organizer
      日本再生医療学会
    • Place of Presentation
      仙台国際センター
    • Related Report
      2016 Annual Research Report
  • [Presentation] in situ reprogrammed spermine treated-adipose tissue–derived multi-lineage progenitor cells improve left ventricular dysfunction in a swine chronic myocardial infarction model.2016

    • Author(s)
      Okura H., Morita M., Fujita M., Naba K., Hasebe-Takada N., Ichinose A., Matsuyama A.
    • Organizer
      Clinical applications of stem cell
    • Place of Presentation
      Singapore
    • Year and Date
      2016-02-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 「再生医療の向こう側」2016

    • Author(s)
      大倉 華雪
    • Organizer
      エレクトロニクス実装学会
    • Place of Presentation
      ラフォーレ琵琶湖
    • Related Report
      2016 Annual Research Report
  • [Presentation] 「再生医療実現化にむけて乗り越えるべき課題」~お力をお貸しくださる方はおられませんか?2016

    • Author(s)
      大倉 華雪
    • Organizer
      エレクトロニクス実装学会
    • Place of Presentation
      ラフォーレ琵琶湖
    • Related Report
      2016 Annual Research Report
  • [Presentation] Allogenic spermine treated-adipose tissue–derived multi-lineage progenitor cells improve left ventricular dysfunction in a swine chronic myocardial infarction model.2015

    • Author(s)
      Okura H., Morita M., Fujita M., Naba K., Hasebe-Takada N., Ichinose A., Matsuyama A.
    • Organizer
      ICCAD
    • Place of Presentation
      Florence, Italy
    • Year and Date
      2015-11-29
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Case Study ACT社 ヒトES由来RPEの品質評価2015

    • Author(s)
      大倉 華雪
    • Organizer
      第12回MCP策定会議
    • Place of Presentation
      大阪
    • Year and Date
      2015-09-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] Case Study ACT社 ヒトES由来RPEの品質評価2015

    • Author(s)
      大倉 華雪
    • Organizer
      第11回MCP策定会議
    • Place of Presentation
      京都
    • Year and Date
      2015-09-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] Case Study ACT社 ヒトES由来RPEの品質評価2015

    • Author(s)
      大倉 華雪
    • Organizer
      第10回MCP策定会議
    • Place of Presentation
      東京
    • Year and Date
      2015-08-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] Case Study ACT社 ヒトES由来RPEの品質評価2015

    • Author(s)
      大倉 華雪
    • Organizer
      第9回MCP策定会議
    • Place of Presentation
      京都
    • Year and Date
      2015-08-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] Therapeutic potential of human adipose tissue-derived multi-lineage progenitor cells in non-alcoholic fatty liver disease Bridging the MOA and the POC for clinical application -in case study.2015

    • Author(s)
      Okura H., Morita M., Fujita M., Naba K., Hasebe-Takada N., Ichinose A., Matsuyama A.
    • Organizer
      国際幹細胞学会
    • Place of Presentation
      Stockholm Sweden
    • Year and Date
      2015-06-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Book] 脂肪組織由来細胞を用いる冠動脈疾患治療戦略2016

    • Author(s)
      大倉華雪・松山晃文
    • Publisher
      日本臨床社
    • Related Report
      2015 Annual Research Report
  • [Book] Critical Path Initiative for Regenerative Medicine in Japan. Gene Therapy and Cell Therapy Through the Liver.2015

    • Author(s)
      Okura H. and Matsuyama A.
    • Total Pages
      7
    • Publisher
      Springer Japan
    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] 凸部を備える細胞培養用プレート及び細胞培養器具2015

    • Inventor(s)
      松山 晃文、大倉 華雪
    • Industrial Property Rights Holder
      国立研究開発法人医薬基盤・健康・栄養研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2015-219783
    • Filing Date
      2015-11-09
    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] 貫通孔を備える細胞培養用プレートを備える積層プレート式細胞培養器具2015

    • Inventor(s)
      松山 晃文、大倉 華雪
    • Industrial Property Rights Holder
      国立研究開発法人医薬基盤・健康・栄養研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2015-219802
    • Filing Date
      2015-11-09
    • Related Report
      2015 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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