Reconstruction of Neuronal Circuitry by Muse cell transplantation in lacunar stroke

• Project/Area Number: 15H05680
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Neurosurgery
• Research Institution: Tohoku University
• Principal Investigator: Sakata Hiroyuki 東北大学, 医学系研究科, 非常勤講師 (80722305)
• Research Collaborator: TOMINAGA Teiji ; DEZAWA Mari ; Chan Pak H. ; WATANABE Mika ; FUJIMURA Miki ; NAKAGAWA Atsuhiro ; NIIZUMA Kuniyasu
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥23,790,000 (Direct Cost: ¥18,300,000、Indirect Cost: ¥5,490,000); Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2016: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000); Fiscal Year 2015: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
• Keywords: 神経再生 / 脳梗塞 / Muse細胞 / 白質再建 / 移植・再生医療 / 脳血管障害学 / 神経科学 / 脳神経疾患 / 再生医学

Outline of Final Research Achievements

Multilineage-differentiating stress-enduring (muse) cells, subpopulation of bone marrow-mesenchymal stem cells, are endogenous　nontumorigenic stem cells with pluripotency. We transplanted Muse cells into the perilesion brain at 2 weeks after lacunar infarction in immunodeficient mice. Transplantation at the delayed subacute phase showed muse cells differentiated into neural cells, facilitated neural reconstruction, improved functions, and displayed solid safety outcomes over prolonged graft maturation period, indicating their therapeutic potential for lacunar stroke.

Research Products

• [Journal Article] Human Muse Cells Reconstruct Neuronal Circuitry in Subacute Lacunar Stroke Model. (2017)
  • Author(s): Uchida H, Niizuma K, Kushida Y, Wakao S, Tominaga T, Borlongan CV, Dezawa M.
  • Journal Title: Stroke. Volume: 48(2) Issue: 2 Pages: 428-35
  • DOI: 10.1161/strokeaha.116.014950
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Transplantation of Unique Subpopulation of Fibroblasts, Muse Cells, Ameliorates Experimental (2016)
  • Author(s): Satow, T., Kawase, T., Kitamura, A. , Kajitani ,Y. , Yamaguchi, T. , Tanabe, N. , Otoi, R. , Komuro, T. , Kobayashi, A. , Nagata, H. , Mima, T.,
  • Journal Title: Stem Cells Volume: 34 Issue: 1 Pages: 160-173
  • DOI: 10.1002/stem.2206
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Hypoxic preconditioning enhances neural stem cell transplantation therapy after intracerebral hemorrhage in mice. (2016)
  • Author(s): Wakai T, Narasimhan P, Sakata H, Wang E, Yoshioka H, Kinouchi H, Chan PH.
  • Journal Title: J Cereb Blood Flow Metab. Volume: 36 Issue: 12 Pages: 2134-2145
  • DOI: 10.1177/0271678x15613798
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Experimental model of small subcortical infarcts in mice with long-lasting functional disabilities. (2015)
  • Author(s): Uchida H, Sakata H, Fujimura M, Niizuma K, Kushida Y, Dezawa M, Tominaga T
  • Journal Title: Brain Res. Volume: 1629 Pages: 318-328
  • DOI: 10.1016/j.brainres.2015.10.039
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant