| Budget Amount *help |
¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2018: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
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| Outline of Final Research Achievements |
We have found that a functional circadian rhythm exists in the urothelium, and it coordinates connexin43 (Cx43) expression and function as hemichannels that provide a direct pathway of ATP release for mechanotransduction and signaling in the urothelium. In addition, we have generated conditional knockout C57BL/6 mice lacking a major clock gene Bmal1 or Cx43 in the urothelium only. Furthermore, we have generated P(Bmal1)-dLuc reporter cell lines derived from immortalized human urothelial cells and have identified a few candidate compounds that could modify the circadian rhythm of the urothelium.
We presently progress the research to investigate the role of peripheral clock in the urothelium for the bladder function and diseases characterized as disorders of micturition rhythm including nocturnal enuresis and nocturia.
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