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Analysis by a new imaging system for three-dimensional detection of nanoscale vibrations in the inner ear

Research Project

Project/Area Number 15H05683
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Otorhinolaryngology
Research InstitutionNiigata University

Principal Investigator

Nin Fumiaki  新潟大学, 医歯学系, 准教授 (80644905)

Research Collaborator 日比野 浩  新潟大学, 医学部 分子生理学, 教授
崔 森悦  新潟大学, 工学部 電気電子工学科, 助教
Lecture Tobias Reichenbach  インペリアルカレッジ
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
Keywordsナノ微小振動 / 蝸牛 / 感覚上皮帯 / 内耳 / ナノ振動計測 / 進行波 / トモグラフィ / 有毛細胞 / 基底板振動 / OCT
Outline of Final Research Achievements

We have developed an imaging system that can detect the object’s nano-scale vibrations. In addition, we succeeded to record a baseline shift of the vibration by a newly derived laser interferometer. Based on the recorded data, we hypothesized that the vibrations depend on the hair cells’ function, and performed mathematical simulation of the sensory epithelia. Lastly, we confirmed the hypothesis by the experiments in which we used animals and applied some drugs in vivo. These results not only show the new mechanism for the vibration of the sensory epithelia, but also found that the mechanism are related to the hari cells’ function for the first time.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (31 results)

All 2018 2017 2016 2015

All Journal Article (9 results) (of which Peer Reviewed: 7 results,  Open Access: 2 results,  Acknowledgement Compliant: 3 results) Presentation (21 results) (of which Int'l Joint Research: 7 results,  Invited: 3 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] 内耳の特殊な電位環境を維持するしくみ(計算科学と実験科学を用いた解析)2018

    • Author(s)
      9)任 書晃、倉智 嘉久、日比野 浩
    • Journal Title

      実験医学

      Volume: 36 Pages: 407-411

    • Related Report
      2017 Annual Research Report
  • [Journal Article] 人工内耳の現状と展望2017

    • Author(s)
      太田 岳、任 書晃、日比野 浩
    • Journal Title

      精密工学会誌

      Volume: 83 Pages: 992-995

    • NAID

      130006855987

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Multifrequency-swept optical coherence microscopy for highspeed full-field tomographyic vibrometry in biological tissues.2017

    • Author(s)
      Choi S, Sato K, Ota T, Nin F, Muramatsu S, Hibino H.
    • Journal Title

      Biomed Opt Express

      Volume: 8 Pages: 608-621

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hearing Loss Controlled by Optogenetic Stimulation of Nonexcitable Nonglial Cells in the Cochlea of the Inner Ear2017

    • Author(s)
      Sato MP, et al.
    • Journal Title

      Frontiers in Molecular Neuroscience

      Volume: 10 Pages: 300-300

    • DOI

      10.3389/fnmol.2017.00300

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Time-controllable Nkcc1 knockdown replicates reversible hearing loss in postnatal mice.2017

    • Author(s)
      Watabe T, Xu M, Watanabe J, Higuchi T, Hori K, Sato MP, Nin F, Hibino H, Ogawa K, Masuda M, Tanaka KF.
    • Journal Title

      Sci Rep

      Volume: 00 Pages: 13605-13605

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The unique ion permeability profile of cochlear fibrocytes and its contribution to establishing their positive resting membrane potential,2016

    • Author(s)
      T. Yoshida, F. Nin, S. Murakami, G. Ogata, S. Urtsuka, S. Choi, T. Nakagawa, H. inohara, S. Komune, Y. Kurachi, H. Hibino
    • Journal Title

      Pflugers Arch.

      Volume: 468 Issue: 9 Pages: 1609-1619

    • DOI

      10.1007/s00424-016-1853-2

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The unique electrical properties in an extracellular fluid of the mammalian cochlea; their functional roles, homeostatic processes, and pathological significance.2016

    • Author(s)
      Nin F, Yoshida T, Sawamura S, Ogata G, Ota T, Higuchi T, Murakami S, Doi K, Kurachi Y, Hibino H.
    • Journal Title

      Pflugers Arch

      Volume: 468 Issue: 10 Pages: 1637-1649

    • DOI

      10.1007/s00424-016-1871-0

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Wide-field heterodyneinterferometricvibrometryfor two-dimensionalsurfacevibrationmeasurement2015

    • Author(s)
      S Choi, , Y Maruyama, T Suzuki, F Nin, H Hibino, O Sasaki
    • Journal Title

      Optics Communications

      Volume: 356 Pages: 343-349

    • DOI

      10.1016/j.optcom.2015.08.016

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Multifrequency swept common-path en-face OCT for wide-field measurement of interior surface vibrations in thick biological tissues2015

    • Author(s)
      S Choi, T Watanabe, T Suzuki, F Nin, H Hibino, O Sasaki
    • Journal Title

      Optics Express

      Volume: 23 Issue: 16 Pages: 21078-21089

    • DOI

      10.1364/oe.23.021078

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 多波長走査型OCTによる生体内振動計測2017

    • Author(s)
      崔 森悦,任 書晃,太田 岳,日比野 浩
    • Organizer
      レーザー学会学術講演会第37回年次大会
    • Place of Presentation
      徳島大学常三島キャンパス(徳島県徳島市)
    • Year and Date
      2017-01-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] Multi-frequency swept en-face optical coherence microscopy with supercontinuum comb for in-vivo measurement of inner ear2017

    • Author(s)
      15)Choi S, Nin F, Ota T, Sato K, Suzuki T, Hibino H.
    • Organizer
      OIE17 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Multi-frequency swept en-face optical coherence microscopy with supercontinuum comb for in-vivo measurement of inner ear2017

    • Author(s)
      16)Choi S, Nin F, Ota T, Sato K, Suzuki T, Hibino H.
    • Organizer
      The 7th Korea-Japan Workshop on Digital Holography and Information Photonics (DHIP2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 多波長走査型OCTによる生体内振動計測.2017

    • Author(s)
      104)崔 森悦、任 書晃、太田 岳、日比野 浩.
    • Organizer
      レーザー学会学術講演会第37回年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 内耳蝸牛に生じるナノ振動計測のためのレーザ干渉計の作製および技術展開2017

    • Author(s)
      107)太田 岳、崔 森悦、任 書晃、日比野 浩
    • Organizer
      第68回日本薬理学会北部会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 内耳ナノ振動を標的としたレーザ干渉計の作製と技術展開2017

    • Author(s)
      109)太田 岳、崔 森悦、任 書晃、日比野 浩
    • Organizer
      第64回中部日本生理学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 生きた感覚上皮帯の 3 次元振動分布計測のためのスーパーコンティニウム多波長走査型 en-face OCT 顕微鏡2017

    • Author(s)
      111)崔 森悦、任 書晃、太田 岳、佐藤 敬太、日比野 浩
    • Organizer
      日本光学会OPJ2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 多波長走査型干渉顕微鏡を用いた広視野in-vivo蝸牛基底板の振動計測2017

    • Author(s)
      114)崔 森悦, 任 書晃, 太田 岳, 佐藤 敬太、日比野 浩
    • Organizer
      応用物理学会春季学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 内耳蝸牛の感覚上皮帯に生じるナノ振動の特性:改良型レーザ干渉計による検討2017

    • Author(s)
      115)太田 岳、崔 森悦、任 書晃、日比野 浩
    • Organizer
      第95回日本生理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 内耳感覚上皮ナノ振動の3次元計測を志向した新規光干渉顕微鏡の創製2017

    • Author(s)
      116)任 書晃、崔 森悦、太田 岳、日比野 浩
    • Organizer
      第95回日本生理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 二重正弦波位相変調法を用いた生体ナノ振動計測技術の開発2016

    • Author(s)
      太田 岳,崔 森悦,任 書晃,日比野 浩
    • Organizer
      第58回光波センシング技術研究会
    • Place of Presentation
      東京理科大学神楽坂キャンパス森戸記念館(東京都新宿区)
    • Year and Date
      2016-12-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] 生体内ナノ振動計測を目指した多波長走査型en-face OCTの開発2016

    • Author(s)
      崔 森悦,任 書晃,太田 岳,日比野 浩
    • Organizer
      第58回光波センシング技術研究会
    • Place of Presentation
      東京理科大学神楽坂キャンパス森戸記念館(東京都新宿区)
    • Year and Date
      2016-12-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] 広視野ヘテロダイン干渉振動計測法による生体表面の高速振動計測2016

    • Author(s)
      崔 森悦, 鈴木 孝昌, 任 書晃, 日比野 浩
    • Organizer
      第77回応用物理学会秋季学術講演会
    • Place of Presentation
      朱鷺メッセ(新潟県新潟市)
    • Year and Date
      2016-09-13
    • Related Report
      2016 Annual Research Report
  • [Presentation] 多波長走査型 en-face 干渉顕微鏡による生体内部の形状計測2016

    • Author(s)
      佐藤 敬太, 任 書晃, 日比野 浩, 崔 森悦
    • Organizer
      第77回応用物理学会秋季学術講演会
    • Place of Presentation
      朱鷺メッセ(新潟県新潟市)
    • Year and Date
      2016-09-13
    • Related Report
      2016 Annual Research Report
  • [Presentation] Wide-field Heterodyen Vibrometry for Measurement of Surface Vibrations in Biological Tissues2016

    • Author(s)
      Choi S, Nin F, Suzuki T, Hibino H
    • Organizer
      IEEE OMN2016
    • Place of Presentation
      Singapore
    • Year and Date
      2016-07-31
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 3D Tomographic Measurement of Interior Surface Vibrations in Thick Biological Tissues Using Multifrequency Sweepable Optical Comb2016

    • Author(s)
      Choi S, Nin F, Sato K, Hibino H
    • Organizer
      2016 Collaborative Conference on 3D & Materials Research (CC3DMR)
    • Place of Presentation
      Seoul (South Korea)
    • Year and Date
      2016-06-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 多波長走査型干渉顕微鏡による生体内部の en-face 断層振動計測2016

    • Author(s)
      崔 森悦、佐藤 敬太、任 書晃、日比野 浩
    • Organizer
      第63回応用物理学会春季学術講演会
    • Place of Presentation
      東京工業大学、東京都、目黒区
    • Year and Date
      2016-03-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] 生体断層計測用GHz 帯Er 添加高調波能動モード同期 ファイバレーザの製作2016

    • Author(s)
      羽田 圭志、崔 森悦、任 書晃、日比野 浩、 黒川 隆志
    • Organizer
      第63回応用物理学会春季学術講演会
    • Place of Presentation
      東京工業大学、東京都、目黒区
    • Year and Date
      2016-03-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] Optical multi-frequency swept sensing for wide-field vibration measurement of interior surfaces in biological tissue.2015

    • Author(s)
      S Choi, F Nin, H Hibino, T Suzuki.
    • Organizer
      SPIE/OSJ Biophotonics Japan 2015
    • Place of Presentation
      筑波大学東京キャンパス、東京都、文京区
    • Year and Date
      2015-10-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Wide-field heterodyne en-face OCT system for vibration measurement of internal surfaces.2015

    • Author(s)
      S Choi, F Nin, T Suzuki, H Hibino.
    • Organizer
      CLEO-PR 2015
    • Place of Presentation
      釜山 韓国
    • Year and Date
      2015-08-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 3D Tomographic Measurement of Interior Surface Vibrations in Thick Biological Tissuese Using Multifrequency Sweepable Optical Comb.2015

    • Author(s)
      S Choi, K Sato, F Nin, H Hibino.
    • Organizer
      2016 Collaborative Conference on 3D and Materials Research (CC3DMR)
    • Place of Presentation
      仁川 韓国
    • Year and Date
      2015-06-20
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Patent(Industrial Property Rights)] 平面振動計測装置及び平面振動計測方法2015

    • Inventor(s)
      崔 森悦
    • Industrial Property Rights Holder
      崔 森悦
    • Industrial Property Rights Type
      特許
    • Filing Date
      2015-06-09
    • Related Report
      2015 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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