Engulfment of apoptotic cells and asymmetry of plasma membranes

• Project/Area Number: 15H05785
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Osaka University
• Principal Investigator: Nagata Shigekazu 大阪大学, 免疫学フロンティア研究センター, 特任教授(常勤) (70114428)
• Project Period (FY): 2015-05-29 – 2020-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥153,530,000 (Direct Cost: ¥118,100,000、Indirect Cost: ¥35,430,000); Fiscal Year 2019: ¥29,250,000 (Direct Cost: ¥22,500,000、Indirect Cost: ¥6,750,000); Fiscal Year 2018: ¥31,070,000 (Direct Cost: ¥23,900,000、Indirect Cost: ¥7,170,000); Fiscal Year 2017: ¥31,070,000 (Direct Cost: ¥23,900,000、Indirect Cost: ¥7,170,000); Fiscal Year 2016: ¥31,070,000 (Direct Cost: ¥23,900,000、Indirect Cost: ¥7,170,000); Fiscal Year 2015: ¥31,070,000 (Direct Cost: ¥23,900,000、Indirect Cost: ¥7,170,000)
• Keywords: アポトーシス / マクロファージ / ホスファチジルセリン / フリッパーゼ / スクランブラーゼ / 自然免疫 / リン脂質 / 貪 食 / 貪食

Outline of Final Research Achievements

Several hundred billion cells die daily in our body. Dead cells expose phosphatidylserine (PtdSer) as an "eat me" signal to their surface. Macrophages recognize PtdSer, and swiftly engulf the dead cells to clear them. In this project, Nagata's group elucidated the molecular mechanism how the PtdSer is exposed to the cell surface during apoptosis, and how macrophages recognize PtdSer to engulf the dead cells. The results obtained in this project will contribute to our understanding of various human diseases such as sepsis and autoimmune disease.

Academic Significance and Societal Importance of the Research Achievements

私達の体内では毎日数十億の細胞が死滅する。この死細胞は速やかにマクロファージによって体内から除去される。この過程が作用しないと死細胞からその内容物 (核やミトコンドリア）が露出、自己免疫疾患を引き起こすと考えられる。一方、この過程が異常に活性化されるとマクロファージによる健康な細胞の貪食、組織破壊が起こると考えられる。本研究成果はこれら自己免疫疾患を理解する上で有用な知見を与える。

Assessment Rating

Verification Result (Rating)

A

Research Products

• [Int'l Joint Research] Sci and Tech Facilities Council (STFC)(英国)
• [Int'l Joint Research] Cincinnati Children's Hospital Med Cent/St. Jude Children's Research Hospital/University of Vermont(米国)
• [Int'l Joint Research] Int Cent for Genet Eng & Biotech (ICGEB)(イタリア)
• [Int'l Joint Research] Hadassah-Hebrew Univ Med Center(イスラエル)
• [Int'l Joint Research] University Medical Center Groningen(オランダ)
• [Int'l Joint Research]
• [Int'l Joint Research] Sci and Tech Facilities Council (STFC)(英国)
• [Int'l Joint Research] Cincinnati Children's Hospital Med Cent/St. Jude Children's Research Hospital/University of Vermont(米国)
• [Int'l Joint Research] Int Cent for Genet Eng & Biotech (ICGEB)(イタリア)
• [Int'l Joint Research] Hadassah-Hebrew Univ Med Center(イスラエル)
• [Int'l Joint Research] University Medical Center Groningen(オランダ)
• [Int'l Joint Research]
• [Int'l Joint Research] Wellcome Trust-MRC Inst of Metabolic Sci(英国)
• [Int'l Joint Research] The Rockefeller University/Yale University School of Medicine/Univ of Texas Health Science Center(米国)
• [Int'l Joint Research] Univ Nacional de Rio Cuarto(アルゼンチン)
• [Int'l Joint Research] McGill University(カナダ)
• [Int'l Joint Research] Heinrich Heine University of Dusseldorf/Univ Hospital Schleswig-Holstein(ドイツ)
• [Int'l Joint Research]
• [Int'l Joint Research] Wellcome Trust-MRC Inst of Metabolic Sci(英国)
• [Int'l Joint Research] National Yang-Ming University(台湾)
• [Int'l Joint Research] Tytgat Inst for Liver and Intestinal Res(オランダ)
• [Int'l Joint Research] New Jersey Medical School/Cincinnati Children's Hospital Res Found(米国)
• [Int'l Joint Research] Heidelberg University(ドイツ)
• [Int'l Joint Research]
• [Journal Article] TIM4 on type 1 DCs mediates uptake of tumor-associated antigens and activation of anti tumor responses in lung tumors (2021)
  • Author(s): 1. Caronni, N., Piperno, G., Simoncello, F., Romano, O., Vodret, S., Yanagihashi, Y., Dress, R., Dutertre, C., Bugatti, M., Bourdely, P., Prete, A., Schioppa, T., Mazza, E., Collavin, L., Zacchigna, S., Ostuni, R., Guermonprez, P., Vermi, W., Ginhoux, F., Bicciato, S., Nagata, S., and Benvenuti, F.
  • Journal Title: Nat.Commun Volume: -
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Tim4 recognizes carbon nanotubes and mediates phagocytosis leading to granuloma formation (2021)
  • Author(s): Omori S, Tsugita M, Hoshikawa Y, Morita M, Ito F, Yamaguchi SI, Xie Q, Noyori O, Yamaguchi T, Takada A, Saitoh T, Toyokuni S, Akiba H, Nagata S, Kinoshita K, Nakayama M
  • Journal Title: Cell reports Volume: 34 Issue: 6 Pages: 108734-108734
  • DOI: 10.1016/j.celrep.2021.108734
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sensing and clearance of apoptotic cells (2021)
  • Author(s): Nagata Shigekazu、Segawa Katsumori
  • Journal Title: Current Opinion in Immunology Volume: 68 Pages: 1-8
  • DOI: 10.1016/j.coi.2020.07.007
  • Peer Reviewed
• [Journal Article] Transport Cycle of Plasma Membrane Flippase ATP11C by Cryo-EM (2020)
  • Author(s): Nakanishi Hanayo、Nishizawa Tomohiro、Segawa Katsumori、Nureki Osamu、Fujiyoshi Yoshinori、Nagata Shigekazu、Abe Kazuhiro
  • Journal Title: Cell Reports Volume: 32 Issue: 13 Pages: 108208-108208
  • DOI: 10.1016/j.celrep.2020.108208
  • Peer Reviewed / Open Access
• [Journal Article] Crystal structure of a human plasma membrane phospholipid flippase (2020)
  • Author(s): Nakanishi Hanayo、Irie Katsumasa、Segawa Katsumori、Hasegawa Kazuya、Fujiyoshi Yoshinori、Nagata Shigekazu、Abe Kazuhiro
  • Journal Title: Journal of Biological Chemistry Volume: 295 Issue: 30 Pages: 10180-10194
  • DOI: 10.1074/jbc.ra120.014144
  • Peer Reviewed / Open Access
• [Journal Article] Flippase and scramblase for phosphatidylserine exposure (2020)
  • Author(s): Nagata Shigekazu、Sakuragi Takaharu、Segawa Katsumori
  • Journal Title: Current Opinion in Immunology Volume: 62 Pages: 31-38
  • DOI: 10.1016/j.coi.2019.11.009
  • Peer Reviewed
• [Journal Article] Infertility Caused by Inefficient Apoptotic Germ Cell Clearance in Xkr8-Deficient Male Mice (2019)
  • Author(s): Yamashita Yahiro、Suzuki Chigure、Uchiyama Yasuo、Nagata Shigekazu
  • Journal Title: Molecular and Cellular Biology Volume: 40 Issue: 3
  • DOI: 10.1128/mcb.00402-19
  • Peer Reviewed
• [Journal Article] Functional Expression of the P2X7 ATP Receptor Requires Eros (2019)
  • Author(s): Ryoden Yuta、Fujii Toshihiro、Segawa Katsumori、Nagata Shigekazu
  • Journal Title: The Journal of Immunology Volume: 204 Issue: 3 Pages: 559-568
  • DOI: 10.4049/jimmunol.1900448
  • Peer Reviewed / Open Access
• [Journal Article] Predominant localization of phosphatidylserine at the cytoplasmic leaflet of the ER, and its TMEM16K-dependent redistribution (2019)
  • Author(s): Tsuji Takuma、Cheng Jinglei、Tatematsu Tsuyako、Ebata Aoi、Kamikawa Hiroki、Fujita Akikazu、Gyobu Sayuri、Segawa Katsumori、Arai Hiroyuki、Taguchi Tomohiko、Nagata Shigekazu、Fujimoto Toyoshi
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 116 Issue: 27 Pages: 13368
  • DOI: 10.1073/pnas.1822025116
  • URL: https://pure.teikyo.jp/en/publications/e62284b2-5567-40aa-b36c-45c5ca281a73
  • Peer Reviewed / Open Access
• [Journal Article] Phosphorylation-mediated activation of mouse Xkr8 scramblase for phosphatidylserine exposure (2019)
  • Author(s): Sakuragi T, Kosako H, Nagata S
  • Journal Title: Proc Natl Acad Sci USA Volume: 116 Issue: 8 Pages: 2907-2912
  • DOI: 10.1073/pnas.1820499116
  • Peer Reviewed / Open Access
• [Journal Article] MERTK tyrosine kinase receptor together with TIM4 phosphatidylserine receptor mediates distinct signal transduction pathways for efferocytosis and cell proliferation (2019)
  • Author(s): Nishi Chihiro、Yanagihashi Yuichi、Segawa Katsumori、Nagata Shigekazu
  • Journal Title: Journal of Biological Chemistry Volume: in press Issue: 18 Pages: 7221-7230
  • DOI: 10.1074/jbc.ra118.006628
  • Peer Reviewed / Open Access
• [Journal Article] Apoptosis and Clearance of Apoptotic Cells. (2018)
  • Author(s): Nagata, S.
  • Journal Title: Annu. Rev. Immunol. Volume: 36 Issue: 1 Pages: 489-517
  • DOI: 10.1146/annurev-immunol-042617-053010
  • Peer Reviewed
• [Journal Article] Efferocytosis and autoimmune disease. (2018)
  • Author(s): Kawano, M. and Nagata, S.
  • Journal Title: Int. Immunol. Volume: 30 Issue: 12 Pages: 551-558
  • DOI: 10.1093/intimm/dxy055
  • Peer Reviewed
• [Journal Article] Phospholipid flippases enable precursor B cells to flee engulfment by macrophages (2018)
  • Author(s): Segawa Katsumori、Yanagihashi Yuichi、Yamada Kyoko、Suzuki Chigure、Uchiyama Yasuo、Nagata Shigekazu
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 115 Issue: 48 Pages: 12212-12217
  • DOI: 10.1073/pnas.1814323115
  • Peer Reviewed
• [Journal Article] The CDC50A extracellular domain is required for forming a functional complex with and chaperoning phospholipid flippases to the plasma membrane (2018)
  • Author(s): Segawa Katsumori、 Sachiko Kurata、Shigekazu Nagata
  • Journal Title: The Journal of biological chemistry Volume: 293 Issue: 6 Pages: 2172-2182
  • DOI: 10.1074/jbc.ra117.000289
  • Peer Reviewed
• [Journal Article] Lupus-like autoimmune disease caused by a lack of Xkr8, a caspase-dependent phospholipid scramblase. (2018)
  • Author(s): Kawano, M. and Nagata, S.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 115 Issue: 9 Pages: 2132-2137
  • DOI: 10.1073/pnas.1720732115
  • Peer Reviewed
• [Journal Article] Single-molecule analysis of phospholipid scrambling by TMEM16F. (2018)
  • Author(s): Watanabe, R., Sakuragi, T., Noji,H. and Nagata, S.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 115 Issue: 12 Pages: 3066-3071
  • DOI: 10.1073/pnas.1717956115
  • Peer Reviewed / Open Access
• [Journal Article] Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018. (2018)
  • Author(s): Galluzzi, L. et al.
  • Journal Title: Cell Death Differ. Volume: 25 Issue: 3 Pages: 486-541
  • DOI: 10.1038/s41418-017-0012-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterization of the scrambling domain of the TMEM16 family (2017)
  • Author(s): Gyobu Sayuri、Ishihara Kenji、Suzuki Jun、Segawa Katsumori、Nagata Shigekazu
  • Journal Title: Proc Natl Acad Sci U S A Volume: 114 Issue: 24 Pages: 6274-6279
  • DOI: 10.1073/pnas.1703391114
  • Peer Reviewed / Open Access
• [Journal Article] Mouse macrophages show different requirements for phosphatidylserine receptor Tim4 in efferocytosis (2017)
  • Author(s): Yanagihashi Yuichi、Segawa Katsumori、Maeda Ryota、Nabeshima Yo-ichi、Nagata Shigekazu
  • Journal Title: Proc Natl Acad Sci U S A Volume: 114 Issue: 33 Pages: 8800-8805
  • DOI: 10.1073/pnas.1705365114
  • Peer Reviewed / Open Access
• [Journal Article] Programmed cell death and the immune system. (2017)
  • Author(s): Nagata S, Tanaka M.
  • Journal Title: Nat Rev Immunol. Volume: 17 Issue: 5 Pages: 333-340
  • DOI: 10.1038/nri.2016.153
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cardiac myofibroblast engulfment of dead cells facilitates recovery after myocardial infarction. (2017)
  • Author(s): Michio Nakaya,Kenji Watari,Mitsuru Tajima,Takeo Nakaya,Shoichi Matsuda,Hiroki Ohara,Hiroaki Nishihara,Hiroshi Yamaguchi,Akiko Hashimoto,Mitsuho Nishida,Akiomi Nagasaka,Yuma Horii,Hiroki Ono,Gentaro Iribe,Ryuji Inoue,Makoto Tsuda,Kazuhide Inoue,Akira Tanaka,Masahiko Kuroda,Shigekazu Nagata,Hitoshi Kurose
  • Journal Title: Journal of Clinical Investigation Volume: 127 Issue: 1 Pages: 383-401
  • DOI: 10.1172/jci83822
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Role of Ca(2+) in the Stability and Function of TMEM16F and 16K. (2016)
  • Author(s): Ishihara K, Suzuki J, Nagata S
  • Journal Title: Biochemistry Volume: 55 Issue: 23 Pages: 3180-3188
  • DOI: 10.1021/acs.biochem.6b00176
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Xkr8 phospholipid scrambling complex in apoptotic phosphatidylserine exposure (2016)
  • Author(s): Suzuki J, Imanishi E, Nagata S.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 113 Issue: 34 Pages: 9509-9514
  • DOI: 10.1073/pnas.1610403113
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Osteopontin in spontaneous germinal centers inhibits apoptotic cell engulfment and promotes autoantibody production in lupus mice. (2016)
  • Author(s): Sakamoto、K., Fukushima、Y., Itoh、K., Matsuda, M., Nagata, S., Minato, N., and Hattori, M.
  • Journal Title: J. Immunol. Volume: 197 Issue: 6 Pages: 2177-2186
  • DOI: 10.4049/jimmunol.1600987
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Exposure of Phosphatidylserine on the Cell Surface. (2016)
  • Author(s): Nagata, S., Suzuki, J., Segawa, K. and Fujii, T.
  • Journal Title: Cell Death Differ Volume: 23 Issue: 6 Pages: 952-961
  • DOI: 10.1038/cdd.2016.7
  • Description: 印刷中
  • Peer Reviewed
• [Journal Article] Cell biology: Killer enzymes tethered. (2016)
  • Author(s): Nagata, S.
  • Journal Title: Nature Volume: 533 Issue: 7604 Pages: 474-476
  • DOI: 10.1038/nature18439
  • Peer Reviewed
• [Journal Article] Human Type IV P-type ATPases that Work as Plasma Membrane Phospholipid Flippases, and Their Regulation by Caspase and Calcium. (2016)
  • Author(s): Segawa K, Kurata S, Nagata S.
  • Journal Title: The Journal of Biological Chemistry Volume: 289 Issue: 2 Pages: 33543-33556
  • DOI: 10.1074/jbc.m115.690727
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] DNA-Mediated Cyclic GMP-AMP Synthase-Dependent and -Independent Regulation of Innate Immune Responses. (2015)
  • Author(s): Kou Motani, Shinji Ito, and Shigekazu Nagata
  • Journal Title: The Journal of Immunology Volume: 194 Issue: 10 Pages: 4914-4923
  • DOI: 10.4049/jimmunol.1402705
  • Peer Reviewed
• [Journal Article] TMEM16F is required for phosphatidylserine exposure and microparticle release in activated mouse platelets. (2015)
  • Author(s): Toshihiro Fujii, Asuka Sakata, Satoshi Nishimura,
  • Journal Title: PNAS. Volume: 112(41) Issue: 41 Pages: 12800-12805
  • DOI: 10.1073/pnas.1516594112
  • Peer Reviewed
• [Journal Article] A role of TMEM16E carrying a scrambling domain in sperm motility. (2015)
  • Author(s): Gyobu S, Miyata H, Ikawa M, Yamazaki D, Takeshima H, Suzuki J, Nagata S.
  • Journal Title: Mol Cell Biol. Volume: 36(4) Issue: 4 Pages: 645-59
  • DOI: 10.1128/mcb.00919-15
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] An Apoptotic 'Eat Me' Signal: Phosphatidylserine Exposure. (2015)
  • Author(s): Segawa K, Nagata S.
  • Journal Title: Trends in cell biology Volume: 25 Issue: 11 Pages: 639-650
  • DOI: 10.1016/j.tcb.2015.08.003
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] リン脂質の膜動態とマクロファージ (2020)
  • Author(s): 瀬川 勝盛
  • Organizer: 第93回日本生化学会大会
  • Invited
• [Presentation] アポトーシスと死細胞の貪食 (2019)
  • Author(s): 長田 重一
  • Organizer: 第40回日本炎症・再生医学会
  • Invited
• [Presentation] アポトーシスと死細胞の貪食 (2019)
  • Author(s): 長田 重一
  • Organizer: 第27回日本Cell Death学会
  • Invited
• [Presentation] フリッパーゼとスクランブラーゼ (2019)
  • Author(s): 長田 重一
  • Organizer: 東京大学理学部生物化学科60周年記念シンポジウム
  • Invited
• [Presentation] 死細胞の貪食と細胞膜の非対称性：フリッパーゼとスクランブラーゼ (2019)
  • Author(s): 長田 重一
  • Organizer: 北海道大学遺伝子病制御研究所セミナー
  • Invited
• [Presentation] 細胞の死と死細胞の貪食 (2019)
  • Author(s): 長田 重一
  • Organizer: 大塚製薬講演会
  • Invited
• [Presentation] Phosphatidylserine-Dependent Efferocytosis and Entosis (2019)
  • Author(s): Shigekazu Nagata
  • Organizer: The 2019 Keystone Symposia on Why So Many Ways to Die? Apoptosis, Necroptosis, Pyroptosis and Beyond
  • Int'l Joint Research / Invited
• [Presentation] Flippases and scramblases for phosphatidylserine-exposure (2019)
  • Author(s): Shigekazu Nagata
  • Organizer: The EMBO Workshop on "Cell Death, Immunity and Inflammation"
  • Int'l Joint Research / Invited
• [Presentation] Phosphatidylserine-dependent efferocytosis and entosis (2019)
  • Author(s): Shigekazu Nagata
  • Organizer: The 27th Euroconference on Apoptosis "Cell Death and Regeneration"
  • Int'l Joint Research / Invited
• [Presentation] Mammalian Scramblases and Flippases at Plasma Membranes (2019)
  • Author(s): Shigekazu Nagata
  • Organizer: Gordon Research Conference on Molecular and Cellular Biology of Lipids
  • Int'l Joint Research / Invited
• [Presentation] Phospholipid Flippase and Scramblase (2019)
  • Author(s): Shigekazu Nagata
  • Organizer: IMS-JSI International Symposium on Immunology 2019
  • Int'l Joint Research / Invited
• [Presentation] Phospholipid Flippase and Scramblase (2019)
  • Author(s): Shigekazu Nagata
  • Organizer: The 60th International Conference on the Bioscience of Lipids
  • Int'l Joint Research / Invited
• [Presentation] Phospholipid Flippase and Scramblase (2019)
  • Author(s): Shigekazu Nagata
  • Organizer: The 2nd Cell Death, Cell Stress and Metabolism Conference
  • Int'l Joint Research / Invited
• [Presentation] Flippase and Scramblase (2019)
  • Author(s): Nagata, S.
  • Organizer: Ken-ichi Arai Memorial Symposium: Five Decades of Pursuit of Science and Human Networks, The University of Tokyo (IMSUT), Tokyo, JAPAN
  • Int'l Joint Research / Invited
• [Presentation] Exposure of phosphatidylserine and engulfment of apoptotic cells by macrophages (2018)
  • Author(s): Nagata, S.
  • Organizer: The 2018 Henry Kunkel Society Meeting, Necker Hospital for Sick Children, Paris, FRANCE
  • Int'l Joint Research / Invited
• [Presentation] Exposure of phospatidylserine on apoptotic cells (2018)
  • Author(s): Nagata, S.
  • Organizer: The AAI annual meeting: Immunology 2018, Austin Convention Center, Austin, USA
  • Int'l Joint Research / Invited
• [Presentation] Phosphatidylserine-dependent efferocytosis (2018)
  • Author(s): Nagata, S.
  • Organizer: 【Keynote Lecture】The Australia-Japan Meeting on Cell Death, Ichijo Hall, The University of Tokyo, Tokyo, JAPAN
  • Int'l Joint Research / Invited
• [Presentation] Engulfment of phosphatidylserine-dependent efferocytosis (2018)
  • Author(s): Nagata, S.
  • Organizer: The International Cell Death Society meeting “About canonical, non-canonical and immunogenic cell death: basic mechanisms and translational applications”, Seoul National University, Seoul, KOREA
  • Int'l Joint Research / Invited
• [Presentation] Cell Death and Fate of Dead Cells (2018)
  • Author(s): Nagata, S.
  • Organizer: 【Invited Lecture】The 5th Kyoto University - Inamori Foundation Joint Kyoto Prize Symposium (KUIP), Yurakucho Asahi Hall, Tokyo, JAPAN
  • Int'l Joint Research / Invited
• [Presentation] hosphatidylserine-depnedent engulfment of apoptotic cells by macrophages (2018)
  • Author(s): Nagata, S.
  • Organizer: The 2018 Gordon Research Conference on Cell Death, Grand Summit Hotel, Sunday River, USA
  • Int'l Joint Research / Invited
• [Presentation] Phosphatidylserine-dependent efferocytosis and entosis (2018)
  • Author(s): Nagata, S.
  • Organizer: The 26th Euroconference on Apoptosis "Cell death in disease: from small molecules to translational medicine", Courtyard St. Petersburg Center West/Pushkin Hotel, St. Petersburg, RUSSIA
  • Int'l Joint Research / Invited
• [Presentation] Phosphatidylserine-dependent efferocytosis and entosis (2018)
  • Author(s): Nagata, S.
  • Organizer: 【Keynote Lecture】EMBO Meeting on Phagocytosis of dying cells: Molecules, mechanisms and therapeutic implications, Thagaste Monastry, Ghent, BELGIUM
  • Int'l Joint Research / Invited
• [Presentation] Phosphatidylserine-dependent efferocytosis and entosis (2018)
  • Author(s): Nagata, S.
  • Organizer: Seminar at IBMM, Universite Libre de Bruxelles, Charleroi, BELGIUM
  • Int'l Joint Research / Invited
• [Presentation] 細胞膜の非対称性 (2018)
  • Author(s): 長田 重一
  • Organizer: 「富山県創薬研究開発センター」開所記念シンポジウム【基調講演】富山国際会議場 富山
  • Invited
• [Presentation] 細胞膜の非対称性 ~ フリッパーゼとスクランブラーゼ ~ (2018)
  • Author(s): 長田 重一
  • Organizer: 金沢大学循環器病態内科学講座【特別講演】ホテル日航金沢 石川
  • Invited
• [Presentation] アポトーシスと病気 (2018)
  • Author(s): 長田 重一
  • Organizer: 第20回日本免疫学会サマースクール 指宿ベイヒルズ 鹿児島
  • Invited
• [Presentation] フォスファチジルセリンに依存した細胞の貪食 (2018)
  • Author(s): 長田 重一
  • Organizer: 第5回日本リウマチ学会 ベーシックリサーチカンファレンス【特別講演】アキバホール 東京
  • Invited
• [Presentation] フォスファチジルセリンを介した細胞の貪食 (2018)
  • Author(s): 長田 重一
  • Organizer: 第21回Osaka Blood Club【特別講演】ホテルグランヴィア大阪 大阪
  • Invited
• [Presentation] 細胞の死と死細胞の運命 (2018)
  • Author(s): 長田 重一
  • Organizer: 兵庫県立明石北高等学校科学講演会 明石北高等学校 兵庫
  • Invited
• [Presentation] Exposure of phosphatidylserine in apoptotic cells. (2018)
  • Author(s): Nagata, S.
  • Organizer: Lecture at the Beutler Institute, Xiamen University, Xiamen, CHINA
  • Int'l Joint Research / Invited
• [Presentation] アポトーシス細胞の貪食 (2018)
  • Author(s): 長田 重一
  • Organizer: 【特別講演】AMED老化メカニズムの解明・制御プロジェクト 第一回リトリート 淡路夢舞台国際会議場 兵庫
  • Invited
• [Presentation] 細胞膜の非対称性 -フリッパーゼとスクランブラーゼ- (2018)
  • Author(s): 長田 重一
  • Organizer: 【招待講演】第18回神経・筋の免疫疾患を考える会 ホテル阪急インターナショナル 大阪
  • Invited
• [Presentation] Exposure of Phosphatidylserine to the Cell Surface. (2017)
  • Author(s): Nagata, S.
  • Organizer: The 2017 Keystone Symposia on Cell Death and Inflammation, Royal Dublin Society, Dublin, IRELAND
  • Int'l Joint Research / Invited
• [Presentation] Exposure of phosphatidylserine during apoptosis and engulfment of dead cells. (2017)
  • Author(s): Nagata, S.
  • Organizer: International Cell Death Society meeting “Cell Death In development and disease”, The Weizmann Institute, Rehovot, ISRAEL
  • Int'l Joint Research / Invited
• [Presentation] From interferons to programmed cell death: My never-ending journey from Zurich. (2017)
  • Author(s): Nagata, S.
  • Organizer: The 50th anniversary of the IMB (Institute of Molecular Biology), IMB University of Zurich, Zurich, SWITZERLAND
  • Int'l Joint Research / Invited
• [Presentation] Exposure of phosphatidylserine to the cell surface, and engulfment of dead cells. (2017)
  • Author(s): Nagata, S.
  • Organizer: International Symposium celebrating the 125th Founding Anniversary of Tokyo University (IMSUT), Tokyo, JAPAN
  • Int'l Joint Research / Invited
• [Presentation] フリッパーゼとスクランブラーゼ (2017)
  • Author(s): 長田 重一
  • Organizer: 【特別講演】平成29年度日本生化学会九州支部例会 宮日会館 宮崎
  • Invited
• [Presentation] 細胞膜の非対称性とその崩壊 - フリッパーゼとスクランブラーゼ (2017)
  • Author(s): 長田 重一
  • Organizer: 【特別講演】第36回日本糖質学会年会 旭川市民文化会館 北海道
  • Invited
• [Presentation] TMEM16ファミリーによるリン脂質スクランブリング機構の解析 (2017)
  • Author(s): 形部小百合、長田重一
  • Organizer: 2017年度生命科学系学会合同年次大会（ConBio2017）神戸国際会議場 神戸
• [Presentation] ホスファチジルセリン受容体Tim4によるTAMレセプター依存的貪食の促進 (2017)
  • Author(s): 柳橋祐一、瀬川勝盛、長田重一
  • Organizer: 2017年度生命科学系学会合同年次大会（ConBio2017）神戸国際会議場 神戸
• [Presentation] Exposure of Phosphatidylserine to the Cell Surface. (2017)
  • Author(s): Nagata, S.
  • Organizer: 【Keynote address】EMBO Workshop on "Cell death, inflammation, and cancer", Innsbruck University Conference Center, Obergurgl, AUSTRIA
  • Int'l Joint Research / Invited
• [Presentation] Asymmetrical Distribution of Phospholipids at Plasma Membrane, and Exposure of Phosphatidylserine to the Cell Surface. (2017)
  • Author(s): 長田 重一
  • Organizer: 【会長特別企画講演】第81回日本循環器学会学術集会 ANAクラウンプラザホテル金沢 金沢
  • Invited
• [Presentation] アポトーシスとフォスファチジルセリンの暴露 (2016)
  • Author(s): 長田 重一
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: パシフィコ横浜 横浜
  • Year and Date: 2016-03-28
  • Invited
• [Presentation] 細胞死と細胞膜の非対称性 (2016)
  • Author(s): 長田 重一
  • Organizer: 名古屋大学創薬科学研究館竣工記念講演会
  • Place of Presentation: 名古屋大学 名古屋
  • Year and Date: 2016-03-03
  • Invited
• [Presentation] Apoptosis and exposure of phosphatidylserine (2016)
  • Author(s): Nagata, S.
  • Organizer: The 5th NIF (Network of Immunology Frontier) Winter School on Advanced Immunology
  • Place of Presentation: The Awaji international Conference Center, Hyogo, JAPAN
  • Year and Date: 2016-01-19
  • Int'l Joint Research / Invited
• [Presentation] Exposure phosphatidylserine in apoptotic cell death, and engulfment of dead cells. (2016)
  • Author(s): Nagata, S.
  • Organizer: The International Meeting on "Cell Death, Inflammation and Immunity", Creta Maris Beach Resort, Creta, GREECE
  • Int'l Joint Research / Invited
• [Presentation] Scramblases and Flippase at Plasma Membrane. (2016)
  • Author(s): Nagata, S.
  • Organizer: Meeting-workshop on Chorea-Acanthocytosis & Vesicular-Vacuolar Transport, University of Michigan, Michigan, USA
  • Int'l Joint Research / Invited
• [Presentation] Exposure of phosphatidylserine to the cell surface. (2016)
  • Author(s): Nagata, S.
  • Organizer: The 3rd Nobel Conference entitled “The Cell Cycle and Cell Death in Disease”, Karolinska Institutet, Stockholm, SWEDEN
  • Int'l Joint Research / Invited
• [Presentation] Exposure of Phosphatidylserine to the Cell Surface. (2016)
  • Author(s): Nagata, S.
  • Organizer: RIKEN IMS Summer Program (RISP 2016), RIKEN-IMS, Yokohama, JAPAN
  • Int'l Joint Research / Invited
• [Presentation] Exposure of Phosphatidylserine to the Cell Surface. (2016)
  • Author(s): Nagata, S.
  • Organizer: The RIKEN IMS-JSI International Symposium on Immunology 2016, Pacifico Yokohama, Yokohama, JAPAN
  • Int'l Joint Research / Invited
• [Presentation] Molecular Mechanism of Apoptotic Phosphatidylserine Exposure. (2016)
  • Author(s): Nagata, S.
  • Organizer: The 2016 Gordon Research Conference on Cell Death, PGA Catalunya Business and Convention Centre, Girona, SPAIN
  • Int'l Joint Research / Invited
• [Presentation] Flippase and Scramblase that regulate phospholipid distribution at plasma membrane. (2016)
  • Author(s): Nagata, S.
  • Organizer: 【Keynote address】The 26th International Complement Workshop, Hotel Nikko Kanazawa, Kanazawa, JAPAN
  • Int'l Joint Research / Invited
• [Presentation] Exposure of Phosphatidylserine to the Cell Surface. (2016)
  • Author(s): Nagata, S.
  • Organizer: 【Keynote address】The 2016 CSI Congress on Immunology, Hefei Fengda International Hotel, Hefei, CHINA
  • Int'l Joint Research / Invited
• [Presentation] Apoptosis and exposure of phosphatidylserine. (2016)
  • Author(s): Nagata, S.
  • Organizer: The 30th Anniversary Symposium of IMB, Academia Sinica, Taipei, TAIWAN
  • Int'l Joint Research / Invited
• [Presentation] Exposure of Phosphatidylserine to the Cell Surface. (2016)
  • Author(s): 長田 重一
  • Organizer: 【特別講演】第68回日本細胞生物学会大会 京都テルサ 京都
  • Invited
• [Presentation] アポトーシスの分子機構の解明とその生理作用 (2016)
  • Author(s): 長田 重一
  • Organizer: 【特別シンポジウム】第75回日本癌学会学術総会 パシフィコ横浜 横浜
  • Invited
• [Presentation] 細胞膜の非対称性とその崩壊 (2016)
  • Author(s): 長田 重一
  • Organizer: 【特別講演】第66回日本薬学会近畿支部総会・大会 大阪薬科大学 大阪
  • Invited
• [Presentation] Structural analysis of a phospholipid scramblase (2016)
  • Author(s): 櫻木崇晴、長田重一
  • Organizer: 第45回日本免疫学会学術集会 沖縄コンベンションセンター 沖縄
• [Presentation] Apoptosis and Exposure of Phosphatidylserine (2015)
  • Author(s): Nagata, S.
  • Organizer: The 29th annual Richard K. Gershon Memorial Lecture at The Yale University
  • Place of Presentation: Yale University, Connecticut, USA
  • Year and Date: 2015-12-10
  • Int'l Joint Research / Invited
• [Presentation] Flippases and Scramblases at Plasma Membranes that Regulate Phosphatidylserine Exposure (2015)
  • Author(s): Nagata, S.
  • Organizer: The 57th American Society of Hematology Annual Meeting
  • Place of Presentation: Orange County Convention Center, Florida, USA
  • Year and Date: 2015-12-05
  • Int'l Joint Research / Invited
• [Presentation] フリッパーゼとスクランブラーゼ (2015)
  • Author(s): 長田 重一
  • Organizer: BMB2015（第38回日本分子生物学会年会・第88回日本生化学会大会 合同大会）
  • Place of Presentation: 神戸ポートピアホテル 兵庫
  • Year and Date: 2015-12-03
  • Invited
• [Presentation] 細胞膜リン脂質スクランブルの分子機構 (2015)
  • Author(s): 鈴木淳、長田重一
  • Organizer: BMB2015（第38回日本分子生物学会年会・第88回日本生化学会大会 合同大会）
  • Place of Presentation: 神戸ポートピアホテル 兵庫
  • Year and Date: 2015-12-03
• [Presentation] カスパーゼによるリン脂質フリッパーゼの切断とホスファチジルセリンの暴露 (2015)
  • Author(s): 瀬川勝盛、長田重一
  • Organizer: BMB2015（第38回日本分子生物学会年会・第88回日本生化学会大会 合同大会）
  • Place of Presentation: 神戸ポートピアホテル 兵庫
  • Year and Date: 2015-12-02
• [Presentation] 膜タンパク質TMEM16ファミリーにおけるカルシウム結合ドメインの同定 (2015)
  • Author(s): 石原健司、長田重一
  • Organizer: BMB2015（第38回日本分子生物学会年会・第88回日本生化学会大会 合同大会）
  • Place of Presentation: 神戸ポートピアホテル 兵庫
  • Year and Date: 2015-12-02
• [Presentation] 膜たんぱく質のTMEM16Eの精子の運動性、受精能への関与 (2015)
  • Author(s): 形部小百合、宮田治彦、伊川正人、長田重一
  • Organizer: BMB2015（第38回日本分子生物学会年会・第88回日本生化学会大会 合同大会）
  • Place of Presentation: 神戸ポートピアホテル 兵庫
  • Year and Date: 2015-12-01
• [Presentation] Exposure of phosphatidylserine during apoptosis and engulfment of dead cells (2015)
  • Author(s): Nagata, S.
  • Organizer: The Cold Spring Harbor Asia on Targeting Cell Death Mechanisms for the Treatment of Human Diseases
  • Place of Presentation: Suzhou Industry Park Conference Center, Suzhou, CHINA
  • Year and Date: 2015-11-13
  • Int'l Joint Research / Invited
• [Presentation] Apoptosis and exposure of phosphatidylserine (2015)
  • Author(s): Nagata, S.
  • Organizer: Cell Symposia: Cell Death and Immunity
  • Place of Presentation: Claremont Hotel & Spa, Berkeley, CA, USA
  • Year and Date: 2015-11-08
  • Int'l Joint Research / Invited
• [Presentation] Exposure of phosphatidylserine during apoptosis and engulfment of dead cells (2015)
  • Author(s): Nagata, S.
  • Organizer: JAPAN-AUSTRALIA meeting on Cell Death
  • Place of Presentation: WEHI, Melbourne, AUSTRALIA
  • Year and Date: 2015-10-21
  • Int'l Joint Research / Invited
• [Presentation] フリッパーゼとスクランブラーゼ (2015)
  • Author(s): 長田 重一
  • Organizer: 第21回国立国際医療研究センター研究所セミナー
  • Place of Presentation: 国立国際医療研究センター研究所 東京
  • Year and Date: 2015-09-28
  • Invited
• [Presentation] 細胞膜へのフォスファチジルセリンの暴露を制御するフリッパーゼとスクランブラーゼ (2015)
  • Author(s): 長田 重一
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 金沢大学 石川
  • Year and Date: 2015-09-15
  • Invited
• [Presentation] Exposure of phosphatidylserine during apoptosis and engulfment of dead cells (2015)
  • Author(s): Nagata, S.
  • Organizer: International Conference of Cancer Immunotherapy and Macrophages 2015
  • Place of Presentation: University of Tokyo, Tokyo, JAPAN
  • Year and Date: 2015-07-11
  • Int'l Joint Research / Invited
• [Presentation] Flippases and Scramblases that Regulate Phosphatidylserine Exposure at the Plasma Membrane. (2015)
  • Author(s): Nagata, S.
  • Organizer: Gordon Conference on Apoptotic Cell Recognition & Clearance
  • Place of Presentation: University of New England, Maine, USA
  • Year and Date: 2015-06-15
  • Int'l Joint Research / Invited
• [Presentation] 細胞死と"eat me"シグナル (2015)
  • Author(s): 長田 重一
  • Organizer: 第57回日本脂質生化学会
  • Place of Presentation: 一橋大学 東京
  • Year and Date: 2015-05-29
  • Invited
• [Book] スクランブラーゼによるホスファチジルセリンの露出「実験医学」Vol.34, No.7(増刊), 68-73頁 (2016)
  • Author(s): 鈴木淳、長田重一
  • Total Pages: 205
  • Publisher: 羊土社
• [Book] リン脂質フリッパーゼとアポトーシス細胞の認識「実験医学」Vol.34, No.7(増刊), 74-80頁 (2016)
  • Author(s): 瀬川勝盛、長田重一
  • Total Pages: 205
  • Publisher: 羊土社
• [Book] Curr. Top. Dev. Biol.(Hermann Steller, eds) (2015)
  • Author(s): Toda, S., Nishi, C., Yanagihashi, Y., Segawa, K. and Nagata, S.
  • Publisher: Elsevier
• [Remarks] 長田重一研究室
  • URL: http://biochemi.ifrec.osaka-u.ac.jp/
• [Remarks] 大阪大学 免疫学フロンティア研究センター 免疫・生化学
  • URL: http://biochemi.ifrec.osaka-u.ac.jp/
• [Patent(Industrial Property Rights)] P2X7受容体発現調節剤 (2020)
  • Inventor(s): 長田重一、領田優太、瀬川勝盛
  • Industrial Property Rights Holder: 長田重一、領田優太、瀬川勝盛
  • Industrial Property Rights Type: 特許
  • Filing Date: 2020
  • Overseas
• [Patent(Industrial Property Rights)] P2X7受容体発現調節剤 (2019)
  • Inventor(s): 長田重一、領田優太、瀬川勝盛
  • Industrial Property Rights Holder: 長田重一、領田優太、瀬川勝盛
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2019-098049
  • Filing Date: 2019
• [Patent(Industrial Property Rights)] ATP11CまたはCDC50Aの阻害物質のスクリーニング方法 (2019)
  • Inventor(s): 長田重一、瀬川勝盛
  • Industrial Property Rights Holder: 長田重一、瀬川勝盛
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2019-082492
  • Filing Date: 2019
• [Patent(Industrial Property Rights)] ATP11CまたはCDC50Aの阻害物質のスクリーニング方法 (2015)
  • Inventor(s): 長田重一、瀬川勝盛
  • Industrial Property Rights Holder: 長田重一、瀬川勝盛
  • Industrial Property Rights Type: 特許
  • Filing Date: 2015
  • Acquisition Date: 2019
• [Patent(Industrial Property Rights)] Method of screening ATP11C or CDC50A inhibitor (2015)
  • Inventor(s): 長田重一、瀬川勝盛
  • Industrial Property Rights Holder: 長田重一、瀬川勝盛
  • Industrial Property Rights Type: 特許
  • Filing Date: 2015
  • Acquisition Date: 2019
  • Overseas
• [Patent(Industrial Property Rights)] Method for Screening Substance Controlling Function of Xkr8 (2015)
  • Inventor(s): 長田重一、鈴木淳
  • Industrial Property Rights Holder: 長田重一、鈴木淳
  • Industrial Property Rights Type: 特許
  • Filing Date: 2015
  • Acquisition Date: 2017
  • Overseas
• [Patent(Industrial Property Rights)] Method of Screening Modulator of Xkr8 (2015)
  • Inventor(s): 長田重一、鈴木淳
  • Industrial Property Rights Holder: 長田重一、鈴木淳
  • Industrial Property Rights Type: 特許
  • Filing Date: 2015
  • Acquisition Date: 2018
  • Overseas