Investigation of calyculin biosynthesis pathway
| Project/Area Number |
15H05996
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Natural medicines
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| Research Institution | Hokkaido University |
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Principal Investigator |
EGAMI Yoko 北海道大学, 薬学研究院, 助教 (50758612)
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | カリクリンA / 生合成 / 薬学 |
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| Outline of Final Research Achievements |
In this study, we tried to investigation of calyculin A biosynthetic pathway. Uncultured symbiont bacteria Candidatus Entotheonella sp. had been identified as calyculin producer. To obtain functional evidences of uncultured bacterial genome, there is no ways other than manipulate gene in cultivable heterologous host, or establish the cultivation condition. To analysis of the biosynthetic mechanisms for nitrile formation, phosphorylation at C17, and chain-shortening process during calyculin biosynthesis, putative tailoring enzymes and substrates were prepared based on bioinfomation of calyculin biosynthetic gene, and subjected to in vitro analyses. As a result, new compound was detected in CalD enzyme reaction.
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Report
(3 results)
Research Products
(2 results)
