Investigation of calyculin biosynthesis pathway

Research Project

Project/Area Number	15H05996
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Natural medicines
Research Institution	Hokkaido University
Principal Investigator	EGAMI Yoko 北海道大学, 薬学研究院, 助教 (50758612)
Project Period (FY)	2015-08-28 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000) Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	カリクリンA / 生合成 / 薬学
Outline of Final Research Achievements	In this study, we tried to investigation of calyculin A biosynthetic pathway. Uncultured symbiont bacteria Candidatus Entotheonella sp. had been identified as calyculin producer. To obtain functional evidences of uncultured bacterial genome, there is no ways other than manipulate gene in cultivable heterologous host, or establish the cultivation condition. To analysis of the biosynthetic mechanisms for nitrile formation, phosphorylation at C17, and chain-shortening process during calyculin biosynthesis, putative tailoring enzymes and substrates were prepared based on bioinfomation of calyculin biosynthetic gene, and subjected to in vitro analyses. As a result, new compound was detected in CalD enzyme reaction.