| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
We have examined if bisphosphonate would affect osteoblasts/osteocytes, and also if diabates mellitus would worsen the histological features of osteocytes and bone matrix using in SDT fatty rats mimicking diabates mellitus. Stimulated emission depletion microscopy successfully visualized three dimensional structures of actin filaments within the cytoplasmic processes of osteocytes. SDT fatty rats revealed broad un-mineralized bone matrix including many irregularly-arranged DMP-1-/OCN-immunopositive osteocytes, as well as many α-SMA-positive/CD31-reactive blood vessels in the diaphyseal bone marrow. Taken together, diabetes mellitus appears to influence the histological aspects of osteocytes and vascular endothelial cells.
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