| Project/Area Number |
15H06007
|
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Human pathology
|
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| Research Institution | Hirosaki University |
|---|
Principal Investigator |
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| Project Period (FY) |
2015-08-28 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 癌 / 胆道 / 癌微小環境 / 時計遺伝子 / 上皮間葉転換 / 胆管癌 / 癌関連線維芽細胞 / 膵癌 |
|---|
| Outline of Final Research Achievements |
Pancreato-biliary cancer is one of the most aggressive malignancies in humans. Its aggressive phenotypes are thought to be associated with invasive tumour growth via epithelial mesenchymal transition. We clarified that in a co-culture model of cholangiocarcinoma and human mesenchymal stem cell lines, carcinoma cells indicate mesenchymal transition by morphological changes and immunohistochemical staining. In addition, using RT-PCR, it was clarified
that the expression of DEC1 is elevated in a co-cultured model, and it was suggested that the cancer microenvironment is formed under the control of the clock gene. Furthermore, we could obtain an image of cancer cells infiltrating the lymph vessel in a cancer microenvironment model (three-dimensional culture model including the lymphatic vessel tissue).
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