| Project/Area Number |
15H06050
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Social dentistry
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| Research Institution | Tohoku University |
|---|
Principal Investigator |
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| Project Period (FY) |
2015-08-28 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 歯周病 / 動脈瘤 / 口腔内細菌 / 歯学 / 細菌 |
|---|
| Outline of Final Research Achievements |
We extracted DNA from the aneurysms samples after beads crush was performed to them. We obtained the DNA solution which tried the DNA amplification of the region that encoded 16rRNA using PCR with obtained DNA solution. As a result, We obtained the DNA contained with not only the bacterial DNA but also the aneurysms wall, we examined annealing temperature, and single band was detected in 55.7 degrees Celsius in 30 times for an amplification cycle when we set it and, furthermore, determined what could completely remove the gram-positive and negative bacteria which lived in what we washed with hypochlorous acid with aneurysms sample before the culture from aneurysms wall to be aneurysmal, and to remove bacterial DNA in the blood which attached.
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