The analysis of lymphocyte function against biofilm formation in chronic wound

• Project/Area Number: 15H06052
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Fundamental nursing
• Research Institution: Tohoku University
• Principal Investigator: TANNO HIROMASA 東北大学, 医学系研究科, 助教 (10755664)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 創傷治癒 / 感染 / リンパ球 / 看護学 / 免疫学 / 創傷治癒学

Outline of Final Research Achievements

Pseudomonas aeruginosa is frequently isolated from chronic wounds.NKT cells play an important role in various　aspects of the regulation immune responses, including protection against bacterial infections. However, it is not fully understood how this lymphocyte is involved in the host defense against P. aeruginosa wound infection. In the present sudy,we elucidated the role of NKT cells in clearance of P. aeruginosa from the wound. NKT cell-deficient mice showed impaired clearance of P. aeruginosa.
These results indicate that NKT cells may be involved in the clearance of P. aeruginosa from wounds.

Research Products

• [Journal Article] IL-17A promotes neutrophilic inflammation and disturbs acute wound healing in skin. (2017)
  • Author(s): Takagi N, Kawakami K, Kanno E, Tanno H, Takeda A, Ishii K, Imai Y, Iwakura Y, Tachi M.
  • Journal Title: Dermatol. Volume: 26 Issue: 2 Pages: 137-144
  • DOI: 10.1111/exd.13115
  • Peer Reviewed / Open Access
• [Journal Article] Reconsideration of Iodine in Wound Irrigation: The Effects on Pseudomonas Aeruginosa Biofilm Formation (2016)
  • Author(s): Emi Kanno, Hiromasa Tanno, Aiko Suzuki, Rina Kamimatsuno, Masahiro Tachi
  • Journal Title: Journal of Wound Care Volume: 25 Issue: 6 Pages: 335-339
  • DOI: 10.12968/jowc.2016.25.6.335
  • Peer Reviewed
• [Presentation] NKT細胞の活性化が創傷治癒に与える影響―α-Galactosylceramide創部投与による検証 (2016)
  • Author(s): 丹野寛大，川上和義, 菅野恵美, 今村彩乃, 佐藤紀子，正木愛梨，高木尚之，石井恵子, 丸山良子, 館正弘
  • Organizer: 第46回日本創傷治癒学会
  • Place of Presentation: 東京 (伊藤国際学術研究センター, 安田講堂)
  • Year and Date: 2016-12-09
• [Presentation] Effect of iNKT cell deficiency on neutrophilic response during early phase of skin wound healing (2016)
  • Author(s): TANNO Hiromasa, KANNO Emi, SATO Noriko, MASAKI Airi, ISHII Keiko, KAWAKAMI Kazuyoshi
  • Organizer: 第45回日本免疫学会学術集会
  • Place of Presentation: 沖縄 (沖縄コンベンションセンター, ラクナガーデンホテル)
  • Year and Date: 2016-12-05
• [Presentation] Lack of iNKT cells leads to persisted infiltration of neutrophils and delayed wound healing process in skin (2016)
  • Author(s): Hiromasa Tanno, Kazuyoshi Kawakami, Emi Kanno, Keiko Ishii, Ryoko Maruyama, Masahiro Tachi
  • Organizer: 5th Congress of the World Union of Wound Healing Societies
  • Place of Presentation: Firenze, Italy
  • Year and Date: 2016-09-25
  • Int'l Joint Research
• [Presentation] Invariant Natural Killer T cells: Roles in skin wound healing and potential clinical application (2016)
  • Author(s): Hiromasa Tanno, Kazuyoshi Kawakami, Emi Kanno, Naoyuki Takagi, Keiko Ishii, Ryoko Maruyama, Masahiro Tachi
  • Organizer: The 10th Congress of Chinese Tissue Repair Society
  • Place of Presentation: Fuzhou, China
  • Year and Date: 2016-09-10
  • Int'l Joint Research / Invited
• [Presentation] Invariant Natural Killer T cells: Roles in skin wound healing and potential clinical application (2016)
  • Author(s): Hiromasa Tanno, Kazuyoshi Kawakami, Emi Kanno, Naoyuki Takagi, Keiko Ishii, Ryoko Maruyama, Masahiro Tachi
  • Organizer: The 13th Japan-Korea Congress of Plastic and Reconstructive Surgery
  • Place of Presentation: 金沢 (ANAクラウンプラザホテル金沢)
  • Year and Date: 2016-05-15
  • Int'l Joint Research / Invited