| Project/Area Number |
15H06078
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Gunma University |
|---|
Principal Investigator |
Hanamura Kenji 群馬大学, 大学院医学系研究科, 助教 (40361365)
|
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| Co-Investigator(Renkei-kenkyūsha) |
TAKATSURU YUSUKE 群馬大学, 大学院医学系研究科, 講師 (30446265)
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|---|
| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 樹状突起スパイン / 2光子励起レーザー顕微鏡 / ドレブリン / NMDA受容体 / in vivo imaging / 神経活動依存性 / 加齢脳 / シナプス機能異常 / アクチン細胞骨格 / アクチン結合タンパク質 |
|---|
| Outline of Final Research Achievements |
In vivo imaging by using two-photon laser microscopy under deep anesthesia revealed dendritic spine morphology of pyramidal neurons in the cerebral cortex of transgenic mice expressing GFP under the control of Thy1-promoter. Analysis of heterozygotes of knockout mice deficient in drebrin A-specific exon or all drebrin isoforms tended to show some changes in turnover rate of dendritic spines compared with wild-type mice. The amount of NMDA receptors in synaptosomes were detected by western blotting.
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