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Development of LC-MS/MS-based accurate quantification methods for assessing serum vitamin D metabolites

Research Project

Project/Area Number 15H06096
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionChiba University

Principal Investigator

ISHIGE Takayuki  千葉大学, 医学部附属病院, 臨床検査技師 (30757315)

Research Collaborator SATOH Mamoru  千葉大学, 医学部附属病院, 特任准教授 (20401002)
NOMURA Fumio  千葉大学, 医学部附属病院, 特任教授 (80164739)
NISHIMURA Motoi  千葉大学, 医学部附属病院, 講師 (80400969)
OGAWA Shoujiro  東京理科大学, 薬学部, 助教 (30546271)
HIGASHI Tatsuya  東京理科大学, 薬学部, 教授 (90272963)
Project Period (FY) 2015-08-28 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
KeywordsビタミンD代謝物 / LC-MS/MS / 誘導体化
Outline of Final Research Achievements

DAPTAD derivatization of vitamin D metabolites were performed for enhancing the ionization efficiency of LC-MS/MS. As compared with 25(OH)D3, the intensity of 25(OH)D3-DAPTAD was increased approximately 100-fold higher. By using SLE for sample preparation, the high-sensitive and robust method for quantification of 4 serum vitamin D metabolites was established. We are planning to establish serum 1,25(OH)2D analysis method by LC/MS/MS.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • Research Products

    (11 results)

All 2016 2015

All Journal Article (4 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 1 results,  Open Access: 1 results) Presentation (7 results)

  • [Journal Article] Locked nucleic acid probe enhances Sanger sequencing sensitivity and improves diagnostic accuracy of high-resolution melting-based KRAS mutational analysis2016

    • Author(s)
      Takayuki Ishige, Sakae Itoga, Kazuyuki Matsushita, Fumio Nomura
    • Journal Title

      Clinica Chimica Acta

      Volume: 457 Pages: 75-80

    • DOI

      10.1016/j.cca.2016.04.005

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development and validation of the simultaneous measurement of four vitamin D metabolites in serum by LC-MS/MS for clinical laboratory applications2016

    • Author(s)
      Mamoru Satoh, Takayuki Ishige, Shoujiro Ogawa, Motoi Nishimura, Kazuyuki Matsushita, Tatsuya Higashi, Fumio Nomura
    • Journal Title

      Anal Bioanal Chem

      Volume: 408 Issue: 27 Pages: 7617-7627

    • DOI

      10.1007/s00216-016-9821-4

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Combined Secretomics and Transcriptomics Revealed Cancer-Derived GDF15 is Involved in Diffuse-Type Gastric Cancer Progression and Fibroblast Activation.2016

    • Author(s)
      Ishige T, Nishimura M, Satoh M, Fujimoto M, Fukuyo M, Semba T, Kado S, Tsuchida S, Sawai S, Matsushita K, Togawa A, Matsubara H, Kaneda A, Nomura F
    • Journal Title

      Sci Rep.

      Volume: 6 Issue: 1 Pages: 21681-21681

    • DOI

      10.1038/srep21681

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Assessment of Vitamin D Metabolism by LC-MS/MS2015

    • Author(s)
      Ishige T, Satoh M, Ogawa S, Nishimura M, Higashi T, Nomura F.
    • Journal Title

      Rinsho Byori

      Volume: 63 Pages: 457-464

    • NAID

      40020474101

    • Related Report
      2015 Annual Research Report
  • [Presentation] サンガー法の検出感度向上のための野生型アレル阻害サイクルシーケンス反応2016

    • Author(s)
      石毛崇之、糸賀栄、西村基、野村文夫、松下一之
    • Organizer
      第23回日本遺伝子診療学会
    • Place of Presentation
      イイノホール&カンファレンスセンター(東京都・千代田区)
    • Year and Date
      2016-10-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] Long-PCRと大量並列シーケンシングによる家族性地中海熱の原因遺伝子MEFVの遺伝学的検査2016

    • Author(s)
      石毛崇之、糸賀栄、北村浩一、西村基、松下一之、野村文夫
    • Organizer
      日本臨床検査自動化学会第48回大会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2016-09-22
    • Related Report
      2016 Annual Research Report
  • [Presentation] Integrated secretome and transcriptome analyses for identification of functional secreted molecules involved in diffuse-type gastric cancer progression2016

    • Author(s)
      Takayuki Ishige, Motoi Nishimura, Mamoru Satoh, Kazuyuki Matsushita, Fumio Nomura
    • Organizer
      The association for Mass Spectrometry: Applications to the Clinical Lab 2016 EU (MSACL2016EU)
    • Place of Presentation
      Salzburg Congress Center, AUSTRIA
    • Year and Date
      2016-09-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] LC/MS/MSによる血清ビタミンD代謝物の精密分析2016

    • Author(s)
      石毛崇之
    • Organizer
      千葉県臨床検査技師会第25回千葉地区研修会
    • Place of Presentation
      千葉メディカルセンター(千葉県・千葉市)
    • Year and Date
      2016-07-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] LC/MS/MSの臨床検査応用 - ビタミンD代謝物測定を基盤に -2015

    • Author(s)
      佐藤守, 石毛崇之, 小川祥二郎, 西村基, 松下一之, 東達也, 野村文夫
    • Organizer
      日本臨床検査自動化学会第47回大会
    • Place of Presentation
      パシフィコ横浜 (神奈川県・横浜市)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] LNA-PCR Sequencingによる高感度KRAS遺伝子変異解析2015

    • Author(s)
      石毛崇之, 糸賀栄, 松下一之, 野村文夫
    • Organizer
      日本臨床検査自動化学会第47回大会
    • Place of Presentation
      パシフィコ横浜 (神奈川県・横浜市)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] LC/MS/MSの臨床検査応用 - ビタミンD代謝物測定を基盤に -2015

    • Author(s)
      佐藤守, 石毛崇之, 小川祥二郎, 西村基, 松下一之, 東達也, 野村文夫
    • Organizer
      第40回日本医用マススペクトル学会年会
    • Place of Presentation
      アクトシティ浜松コングレスセンター (静岡県・浜松市)
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report

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Published: 2015-08-26   Modified: 2018-03-22  

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