Intracellular ATP- and redox potential-responsive siRNA delivery carrier

• Project/Area Number: 15H06108
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: The University of Tokyo
• Principal Investigator: NAITO MITSURU 東京大学, 大学院医学系研究科(医学部), 特任研究員 (50755329)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: siRNA / 環境応答性 / 高分子ミセル / 還元環境 / ATP / DDS / ナノマシン / ナノバイオ / ATP応答性 / 還元環境応答性

Outline of Final Research Achievements

To enhance an efficacy of cancer cell specific siRNA delivery, novel siRNA-loaded polyion complex micelles with intracellular-responsive siRNA releasability has been developed. A cyclic peptide which can selectively bind to cancer cell membrane proteins was introduced onto PIC micellar surface to enhance the targetability. A phenylbotronic acid and thiol moiety were introduced into PIC micellar core to increase PIC micellar stability at extracellular condition and release siRNA into cytoplasm in response to intracellular condition. The newly prepared stimuli-responsive PIC micelles did release siRNA under intracellular mimic condition.

Research Products

• [Presentation] 細胞内へのsiRNA送達を目指したATP応答性PICミセルの機能評価 (2017)
  • Author(s): 内藤瑞
  • Organizer: 第66回高分子学会年次大会
  • Place of Presentation: 幕張メッセ (千葉県千葉市)
  • Year and Date: 2017-05-29
• [Presentation] ATP-responsive polyion complex micelles for intracellular delivery of siRNA (2016)
  • Author(s): 内藤瑞
  • Organizer: 3rd International Conference on Biomaterials Science in Tokyo
  • Place of Presentation: 東京大学 (東京都文京区)
  • Year and Date: 2016-11-28
  • Int'l Joint Research