| Project/Area Number |
15H06158
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 化膿レンサ球菌 / 好中球細胞外トラップ / DNase / Streptococcus pyogenes / Group A streptococcus / NETs |
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| Outline of Final Research Achievements |
We analyzed the whole-genome sequences of Streptococcus pyogenes that was used in our preliminary experiments. Based on bioinformatics analysis, we identified 3 genes coding DNases (SdaD2, endA, spd) and novel 2 DNA regions coding 5'-nucleotidase that potentially degrade neutrophil extracellular traps (NETs) to yield NETs degradation product (NETDP). We have succeeded to generate deletion mutants of 4 genes of them. The DNase activity analysis showed that the strains still possessed substantial DNase activities. We also investigated the effect of the residual bacteria in the assay system prepared from supernatants of NETs co-cultivated with bacteria. Now we are trying to establish deletion mutants of dual or triple genes of the targets to analyze the effect of NETDP production.
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