Multipotency of HSCs and midbody movement

• Project/Area Number: 15H06163
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Hematology
• Research Institution: The University of Tokyo
• Principal Investigator: TANAKA YOSUKE 東京大学, 医科学研究所, 助教 (10509087)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 造血幹細胞 / Midbody / 非対称分裂 / 細胞・組織

Outline of Final Research Achievements

We established a novel Cre-inducible midbody reporter mouse line. Midbody is a unique structure that connects two daughter-cells at the end of cell division. MgcRacGAP-hmKO2 fusion protein was used as a surrogate marker for midbody. To visualize midbody behavior in hematopoietic system, we crossed the Cre-inducible midbody reporter mouse line with Vav-Cre mouse line that expresses Cre in hematopoietic lineage. Hematopoietic stem cells from the mouse line nicely expressed MgcRacGAP-hmKO2 reporter during cell division. We found that hematopoietic stem cells released midbody with higher probability than progenitor cells did. We are going to examine if this is also the case in vivo in the future.