Research of oxidative stress tolerance mechanism in head and neck cancer using glutathione-sensitive fluorescent probes

Research Project

Project/Area Number	15H06175
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Otorhinolaryngology
Research Institution	The University of Tokyo
Principal Investigator	Yoshida Masafumi 東京大学, 医学部附属病院, 講師 (80396754)
Research Collaborator	URANO Yasuteru
Project Period (FY)	2015-08-28 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000) Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	頭頸部癌 / 酸化ストレス / 蛍光イメージング
Outline of Final Research Achievements	We developed fluorescent probe targeted for glutathione (GSH). To overcome the problems of existing probes, we developed the FRET type fluorescent probe, nameed QuicGSH series, using a reversible fluorescence control mechanism. In addition to the quantification of GSH in cultured cells , new findings on oxidative stress tolerance mechanism were achieved. Based on the findings obtained in this study, further elucidate the resistance to treatment of cancer cells and application for cancer therapy are expected.

Report

(3 results)