| Project/Area Number |
15H06188
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
Fujita Koji 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (80451970)
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 成熟骨芽細胞 / 骨粗鬆症 / ヒト成熟骨芽細胞 / 骨折治療 / 骨芽細胞 / 骨形成 |
|---|
| Outline of Final Research Achievements |
The aim of our research is to develop a new strategy of fracture healing by using human mature osteoblast, and in this project we finished a couple of steps. First, we modified the protocol of osteoblast isolation by using new collagenase. With this, the efficacy of isolation increased 2-3 times. Second, we analyzed the effect of donors' disease on gene expression of osteoblast. As a result, we found diabetes changed the gene expression siginificantly. Finally, we started the experiments of transplant of human mature osteoblast to rat model to evaluate the bone formation ability.
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