| Project/Area Number |
15H06235
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Pain science
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Research Collaborator |
Hayashi Katsuhiro 金沢大学, 附属病院, 助教
Tada Kaoru 金沢大学, 医学系, 助教
Yamamoto Daiki 金沢大学, 附属病院, 医員
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 末梢神経再生 / 脂肪由来幹細胞 / 人工神経 / 再生医学 |
|---|
| Outline of Final Research Achievements |
It is currently controversial whether using mesenchymal stem cells differentiated into Schwann cell-like cells is a superior method of promoting regeneration of peripheral nerves. Here, we report the efficacy of using differentiated adipose-derived stem cells (d-ADSCs) to regenerate peripheral nerves.In this study, we used 12-14 week-old female Wistar rats. Adipose tissue was excised from their inguinal region, and the stromal vascular fraction (SVF) was separated by centrifugation. The SVF was cultured for a number of passages into ADSCs, and these cells were then differentiated into d-ADSCs. The left sciatic nerve was resected at a length of 15 mm. This defect was bridged by either a PGA-c tube (Group C), a PGA-c tube into which d-ADSCs had been injected (Group D), or by autologous nerve graft (Group A). In conclusion, regeneration was promoted by d-ADSCs by promoting peripheral nerve regeneration through neuron protection, and by participating in myelination.
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