Development of simple primary screening method for methamphetamine based on electrochemiluminescence

• Project/Area Number: 15H06243
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Legal medicine
• Research Institution: Shinshu University
• Principal Investigator: TAKAHASHI Fumiki 信州大学, 先鋭領域融合研究群環境・エネルギー材料科学研究所, 助教(特定雇用) (40754958)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 電気化学発光 / メタンフェタミン / 電位変調ボルタンメトリー / 電気化学 / 電位変調ボルタンメトリ / 社会医学 / 分析化学 / 覚せい剤

Outline of Final Research Achievements

A primary screening method for methamphetamine (MA) as a stimulant drug was studied by electrochemiluminescence (ECL). The obviously ECL responses was measured in the presence of MA in sample solution when ruthenium complex was used as ECL probe. In order to observe the ECL reaction mechanism, the typical amphetamine type drugs were systematically measured. By the measurements, a simple and rapid screening method for MA is proposed in this study, and was applied in the determination of MA in biological samples with sensitivity based on potential modulation techniques.

Research Products

• [Presentation] 電位変調法を利用した電気化学発光によるメタンフェタミンの高感度検出法の開発と応用 (2016)
  • Author(s): 髙橋史樹, 清水亮, 新田咲, 金継業
  • Organizer: 日本法科学技術学会第22回学術集会
  • Place of Presentation: 中野サンプラザ(東京都・中野区)
  • Year and Date: 2016-11-10
• [Presentation] 水/有機溶媒混合溶液中におけるルテニウム錯体(Ru(bpy)32+)/メタンフェタミン系の電気化学発光挙動の解析 (2016)
  • Author(s): 髙橋史樹, 清水亮, 新田咲, 金継業
  • Organizer: 日本分析化学会第65年会
  • Place of Presentation: 北海道大学(北海道・札幌市)
  • Year and Date: 2016-09-14
• [Presentation] 電位変調-電気化学発光法を利用したメトキシフェナミン鎮咳成分の高感度検出法の開発と応用 (2016)
  • Author(s): 新田咲, 清水亮, 金継業, 髙橋史樹
  • Organizer: 日本分析化学会第65年会
  • Place of Presentation: 北海道大学(北海道・札幌市)
  • Year and Date: 2016-09-14
• [Presentation] Development of Primary Screening Method for Methamphetamine (MA) Based on Ectrochemiluminescence (ECL) and Characterization of Ru(bpy)32+/MA ECL System (2016)
  • Author(s): Fumiki Takahashi, Saki Nitta, Hirosuke Tatsumi, Jiye Jin, Teruo Hinoue
  • Organizer: 54th Annual meeting of the International Association of Forensic Toxicologists
  • Place of Presentation: Brisbane (Australia)
  • Year and Date: 2016-08-28
  • Int'l Joint Research
• [Presentation] Development of Primary Screening Method for Methamphetamine (MA) Based on Electrochemiluminescence (ECL) and Characterization of Ru(bpy)32+/MA ECL System (2016)
  • Author(s): Fumiki Takahashi, Saki Nitta, Hirosuke Tatsumi, Jiye Jin, Teruo Hinoue
  • Organizer: 54th Annual meeting of the International Association of Forensic Toxicologists (TIAFT)
  • Place of Presentation: Brisban Convention & Exhibition Centre (Brisban, Australia)
  • Year and Date: 2016-08-28
  • Int'l Joint Research
• [Presentation] 電気化学発光 (ECL)を利用したメタンフェミンのスクリーニング検査法の開発とECL反応機構の解析 (2016)
  • Author(s): 髙橋史樹・新田咲・巽広輔・金継業・樋上照男
  • Organizer: 日本法中毒学会第35年会
  • Place of Presentation: 大阪産業創造館(大阪市・大阪府)
  • Year and Date: 2016-07-01
• [Presentation] 電気化学発光(ECL)を利用したメタンフェタミンのスクリーニング検査法の開発とECL反応機構の解析 (2016)
  • Author(s): 髙橋史樹, 新田咲, 巽広輔, 金継業, 樋上照男
  • Organizer: 日本法中毒学会第35年会
  • Place of Presentation: 大阪産業創造館(大阪府・大阪市)
  • Year and Date: 2016-06-30
• [Presentation] ルテニウム錯体（Ru(bpy)32+）を用いたメトキシフェナミン鎮咳成分の電気化学及び電気化学発光挙動の解析 (2016)
  • Author(s): 新田咲, 巽広輔, 金継業, 樋上照男, 髙橋史樹
  • Organizer: 第76回日本分析化学討論会
  • Place of Presentation: 岐阜薬科大学・岐阜大学(岐阜県・岐阜市)
  • Year and Date: 2016-05-28
• [Presentation] ルテニウム錯体(Ru(bpy)32+)を用いたメトキシフェナミン鎮咳成分の電気化学及び電気化学発光挙動の解析 (2016)
  • Author(s): 新田 咲・巽広輔・金継業・樋上照男・髙橋 史樹
  • Organizer: 日本分析化学会第76回分析化学討論会
  • Place of Presentation: 岐阜薬科大学・岐阜大学(岐阜県・岐阜市)
  • Year and Date: 2016-05-28
• [Presentation] Effect of Ultrasound on Electrochemiluminescence of Tris(2,2’-bipiridine)ruthenium with Various Coreactants (2015)
  • Author(s): Fumiki Takahashi, Masanori Matsuoka, Jiye Jin
  • Organizer: The 36th Symposium on Ultrasonic Electronics (USE2015)
  • Place of Presentation: Epocal Tsukuba(Ibaraki, Japan)
  • Year and Date: 2015-11-05
  • Int'l Joint Research
• [Presentation] 電気化学発光（ECL）を利用した覚せい剤の選択検出法の開発とECL反応機構の解析 (2015)
  • Author(s): 高橋 史樹・新田 咲・北野 拓磨・巽 広輔・金 継業・樋上 照男
  • Organizer: 日本分析化学会第64年会
  • Place of Presentation: 九州大学(福岡県・福岡市)
  • Year and Date: 2015-09-09
• [Patent(Industrial Property Rights)] 薬物の検出方法 (2017)
  • Inventor(s): 髙橋史樹、金継業
  • Industrial Property Rights Holder: 信州大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-032781
  • Filing Date: 2017-02-24