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Construction of a model symbiotic culture system of wood rot fungi and bacteria, and elucidation of its interaction mechanisms

Research Project

Project/Area Number 15H06252
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Environmental agriculture(including landscape science)
Research InstitutionShizuoka University

Principal Investigator

mori toshio  静岡大学, 農学部, 助教 (80536516)

Project Period (FY) 2015-08-28 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords木材腐朽菌 / 細菌 / 相互作用 / 木材腐朽 / 共培養 / リグニン分解 / 細菌叢 / 防カビ剤
Outline of Final Research Achievements

In order to elucidation of interaction mechanisms between wood-rot fungi and bacteria during wood decay, we aimed to construct of fungi-bacteria model co-culture systems with lower bacterial diversity. Firstly, bacteria that can coexist with a wood rot fungus were penetrated into the fungal bed from natural environment. Then, a model co-culture system showing superior wood decay characteristics than in the case of pure fungal culture was obtained, by repeating subcultivation of contaminated fungal beds. Suppression of filamentous fungi growth that negatively affects wood decay, and sufficient cultivation period were important for construction of a model culture system.
Finally, a model co-culture showing stably superior lignin degradation was obtained. Furthermore, we are searching for the bacterial genes replying to the fungal metabolites extracted from the pure fungal culture by using metagenomic techniques.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • Research Products

    (1 results)

All 2015

All Presentation (1 results)

  • [Presentation] 高活性リグニン分解菌Phanerochaete sordida YK-624株と自然界由来細菌の共培養系の構築2015

    • Author(s)
      森智夫、河岸洋和、平井浩文
    • Organizer
      第60回リグニン討論会
    • Place of Presentation
      筑波大学・茨城県つくば市
    • Year and Date
      2015-11-05
    • Related Report
      2015 Annual Research Report

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Published: 2015-08-26   Modified: 2018-03-22  

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