Development of thermo-responsive magnetic conjugates designed for isolation and purification of circulating tumor cells
| Project/Area Number |
15H06260
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Nanomaterials engineering
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| Research Institution | Nagoya Institute of Technology |
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Principal Investigator |
Fuchigami Teruaki 名古屋工業大学, 工学(系)研究科(研究院), 助教 (20756704)
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 磁性ナノ粒子 / 磁気細胞分離 / 血中循環腫瘍細胞 / 温度応答性高分子 / がん早期診断 / 温度応答性磁性粒子 / ナノ複合構造 / 有機無機ハイブリッド / 機能性ナノ粒子 / がん診断 / 細胞回収 / 血管循環腫瘍細胞 / 細胞回収率 |
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| Outline of Final Research Achievements |
Novel thermo-responsive magnetic conjugates composed of iron oxide particles, streptavidin and thermos-responsive polymer were developed to achieve high-efficiency enrichment and purification of circulating tumor cells (CTCs) with no stress. Controlling an amount of streptavidin on a surface of the conjugates, capture efficiency of CTCs increased from 25 to 60%. Observing the conjugates motion on lung cancer cells under microscope, the thermo-responsive conjugates captured and released cancer cells by changing temperature from body temperature (37°C) to lower temperatures. According to these results, the novel thermos-responsive conjugates has an advantageous for the high-efficiency enrichment and the purification.
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Report
(3 results)
Research Products
(7 results)
