A study of anti-tumor effect of the dendritic cells infected with HF10-packaged amplicon carrying tumor associated antigen

• Project/Area Number: 15H06279
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Nagoya University
• Principal Investigator: Luo Chenhong 名古屋大学, 医学系研究科, 招へい教員 (40759627)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: Glypican 3 / dendritic cells / Lamp-1 / signal peptide / アンプリコン / GCP / LAMP / 樹状細胞 / ウイルス / 腫瘍 / HF10 / GPC3

Outline of Final Research Achievements

In this study, our results indicated that 1. HF10-package amplicon, which expresses tumor associated antigen glypican3 (GPC3), induced the antigen specific immune response through infected dendritic cells and inhibited the tumor growth in mouse ovarian cancer model. 2. In mouse peritoneal tumor seeding model, through prophylactic vaccination, we found that the dosing i.p. had the best antitumor effect among i.p., i.v., and s.c. dosing routes. 3. The chimera of Lamp-1 signal peptide and GPC3 induced GPC3 specific immune response through dendritic cell presentation more strongly than GCP3.

Research Products

• [Presentation] Most recent clinical trial data using HF10 for 9 patients with non resectable pancreatic cancer (2016)
  • Author(s): Yoshinori Naoe, Toru Ichinose, Chenhong Luo, Wu Zhiwen, Nobuaki Mukoyama, Noriyuki Miyajima, Daishi Morimoto, Maki Tanaka, Hideki Kasuya
  • Organizer: The 10th International Meeting on Replicating Oncolytic Virus Therapeutics
  • Place of Presentation: バンクーバー（カナダ)
  • Year and Date: 2016-10-01
  • Int'l Joint Research
• [Presentation] Systemic delivery strategy of oncolytic herpes simplex virus HF10 adsorbed on antigen-specific lymphocytes (2016)
  • Author(s): Toru Ichinose, Chenhong Luo, Yusuke Koide, Wu Zhiwen, Nobuaki Mukoyama, Noriyuki Miyajima, Daishi Morimoto, Suguru Yamada, Tsutomu Fujii, Hiroyuki Sugimoto, Maki Tanaka, Yasuhiro Kodera, Hideki Kasuya
  • Organizer: 第22回日本遺伝子細胞治療学会学術集会
  • Place of Presentation: 虎ノ門ヒルズフォーラム（東京都港区）
  • Year and Date: 2016-07-28
• [Presentation] ENHANCED ANTI-TUMORAL ACTIVITY OF ONCOLYTIC HERPES SIMPLEX VIRUS HF10 WITH CETUXIMAB AGAINST HUMAN COLORECTAL CANCER (2016)
  • Author(s): Zhiwen Wu, Toru Ichinose, Chenhong Luo, Suguru Yamada, Tsutomu Fujii, Hiroyuki Sugimoto, Noriyuki Miyajima, Daishi Morimoto, Nobuaki Mukoyama, Yoko Nishikawa, Usuke Koide, Toshie Kuwahara, Yasuhiro Kodera, Maki Tanaka, Hideki Kasuya
  • Organizer: 第22回日本遺伝子細胞治療学会学術集会
  • Place of Presentation: 虎ノ門ヒルズフォーラム（東京都港区）
  • Year and Date: 2016-07-28
• [Presentation] Combination therapy of squamous cell carcinoma mouse models with PD-L1 immune checkpoint inhibitor and oncolytic herpes simplex virus HF10 (2016)
  • Author(s): Nobuaki Mukoyama, Toru Ichinose, Chenhong Luo, Yusuke Koide, Wu Zhiwen, Noriyuki Miyajima, Daishi Morimoto, Maki Tanaka, Yasushi Fujimoto, Michihiko Sone, Yasuhiro Kodera, Hideki Kasuya
  • Organizer: 第22回日本遺伝子細胞治療学会学術集会
  • Place of Presentation: 虎ノ門ヒルズフォーラム（東京都港区）
  • Year and Date: 2016-07-28
• [Presentation] 腫瘍溶解性ヘルペスウイルスHF10を使用した新規がん治療法の研究 (2016)
  • Author(s): 粕谷英樹、山村和生、藤井努、堀田佳宏、西山幸廣、小出悠介、田中舞紀 、駱晨虹、一ノ瀬亨、宮嶋則行、向山宣昭、中尾昭公、小寺泰弘
  • Organizer: 第116回日本外科学会定期学術集会
  • Place of Presentation: 大阪国際会議場・リーガロイヤルホテル大阪（大阪府大阪市）
  • Year and Date: 2016-04-14
• [Presentation] Systemic virotherapy using oncolytic herpes simplex virus adsorbed onto antigenspecific lymphocytes (2015)
  • Author(s): Toru Ichinose，Akiyuki Kanzaki，Luo Chenhong，Yoshihiro Hotta，Koide Yusuke， Wu Zhiwen， Saori Fukuda，Toshie Kuwahara，Suguru Yamada，Tsutomu Fujii，Hiroyuki Sugimoto，Maki Tanaka，Yasuhiro Kodera，Hideki Kasuya
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡国際会議場 （福岡県福岡市）
  • Year and Date: 2015-11-22
• [Presentation] Anti-tumor immunity with locally administered HF10 effects to the liver and peritoneal metastasis in mouse models (2015)
  • Author(s): Hideki Kasuya, Yoshihiro Hota, Chenhong Luo, Toru Ichinose Zhiwen Wu, Noriyuki Miyazima, Nobuaki Mukouyama, Yusuke Koide , Maki Tanaka , Yasuhiro Kodera
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場 （愛知県名古屋市）
  • Year and Date: 2015-10-08
• [Presentation] Combination therapy against human pancreatic cancer with oncolytic herpes virus hrR3 and monoclonal antibody bevacizumab (2015)
  • Author(s): Zhiwen Wu, Toru Ichinose, Chenhong Luo, Gewen Tan, Tevfik T. Sahin, Suguru Yamada, Tsutomu Fujii, Hiroyuki Sugimoto, Yoko Nishikawa, Usuke Koide, Saori Fukuda, Yasuhiro Kodera, Hideki Kasuya
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場 （愛知県名古屋市）
  • Year and Date: 2015-10-08
• [Remarks] 名古屋大学大学院医学系研究科 癌免疫治療研究室 研究業績
  • URL: https://www.med.nagoya-u.ac.jp/intlexch/cancerimmuno/www/achievements/index.html